Detection of congenital cytomegalovirus infection using umbilical cord blood samples in a screening survey

2009 ◽  
Vol 81 (10) ◽  
pp. 1773-1776 ◽  
Author(s):  
Takeshi Endo ◽  
Kenji Goto ◽  
Koichi Ito ◽  
Tokio Sugiura ◽  
Koji Terabe ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
Blood Samples ◽  
Screening Survey

scholarly journals Is low birth weight a risk indicator for congenital cytomegalovirus infection?

2009 ◽  
Vol 4 (01) ◽  
pp. 044-047 ◽  
Author(s):  
Zakyieh Al-Hareth ◽  
Fawza Monem ◽  
Nagwa Abdel Megiud
Keyword(s):  
Birth Weight ◽  
Low Birth Weight ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Head Circumference ◽  
Cytomegalovirus Infection ◽  
Congenital Infection ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus

Background: Congenital cytomegalovirus infection is currently the leading cause of congenital infection in 0.2-2.2% of live births worldwide leading to variable serious sequalae. The aim of the study was to determine if low birth weight is an indicator of CMV congenital infection evidenced by detecting CMV-DNA in umbilical cord blood at the time of delivery. Methodology: CMV-IgG and IgM antibodies and CMV-DNAemia were assessed in umbilical cord blood of two hundreds newborns, one hundred of whom had birth weight ≤ 2700 gram and/or head circumference ≤ 32 cm. Results: CMV-IgM was not detected, while CMV-IgG was positive in 80-90% of the two hundreds tested newborns. CMV-DNA was detected in four out of the 200 newborns. One of them was over the adopted weight limit (> 2700 gram). Conclusions: CMV-IgM and IgG antibodies assessment was not a potential discriminative test to identify congenitally infected newborns. In addition, low birth weight and small head circumference at birth failed to predict congenital CMV infection. CMV-DNA detection in umbilical cord blood at the time of delivery using real-time PCR of all newborns is recommended as decisive, rapid and non-invasive test.

Production of dendritic cells and cytokine-induced killer cells from banked umbilical cord blood samples

2015 ◽  
Vol 2 (11) ◽  
Author(s):  
Phuc Van Pham ◽  
Binh Thanh Vu ◽  
Viet Quoc Pham ◽  
Phong Minh Le ◽  
Hanh Thi Le ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Killer Cells ◽  
Blood Samples

Acute maternal cytomegalovirus infection is associated with significantly decreased numbers of CD34+ cells in umbilical cord blood

2012 ◽  
Vol 49 (3-4) ◽  
pp. 166-169 ◽  
Author(s):  
José C. Jaime-Pérez ◽  
Julia E. Colunga-Pedraza ◽  
Roberto Monreal-Robles ◽  
Perla R. Colunga-Pedraza ◽  
Nereida Méndez-Ramírez ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cytomegalovirus Infection ◽  
Cd34 Cells

Severe fetal anaemia caused by congenital cytomegalovirus infection

2021 ◽  
Vol 14 (10) ◽  
pp. e244585
Author(s):  
Claudia Salazar-Sanchez ◽  
Pedro Llancarí ◽  
Rommy H Novoa ◽  
Walter Ventura
Keyword(s):  
Pregnant Woman ◽  
Fetal Growth ◽  
Umbilical Cord Blood ◽  
Cytomegalovirus Infection ◽  
Peak Systolic Velocity ◽  
Cmv Infection ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
Fetal Medicine ◽  
Fetal Infection

A 22-year-old pregnant woman was referred to our fetal medicine unit due to severe fetal growth restriction at 26 weeks of gestation. An extensive detailed ultrasound revealed signs of bilateral periventricular hyperechogenicity, suggesting fetal infection potentially due to cytomegalovirus (CMV). Doppler ultrasound showed a high peak systolic velocity in the middle cerebral artery. Percutaneous umbilical cord blood sampling confirmed fetal CMV infection and severe fetal anaemia. We present this case to highlight the importance of fetal anaemia, which can be fatal regardless of whether it is associated with generalised oedema or hydrops fetalis.

Chromatographic Separation Of Fetal and Adult Hemoglobins For Nitric Oxide Quantification In Term Human Umbilical Cord Blood

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 2192-2192
Author(s):  
Daniel A. Riccio ◽  
Brendan Huang ◽  
Brian C. Antczak ◽  
Kristin W. Weaver ◽  
Amy P. Murtha ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Lower Percentage ◽  
Human Umbilical Cord ◽  
Early Detection Of Disease ◽  
Blood Samples ◽  
Significant Difference ◽  
Hb Variant

Abstract Introduction Nitric oxide (NO) is a vasoactive molecule that can bind to hemoglobin (Hb) in the form of S-nitrosothiol (SNO) functionalities at the β93 cysteine residues. Red blood cells (RBCs) containing S-nitrosohemoglobin (SNO-Hb) are able to not only deliver oxygen, but release vasodilatory NO/SNO equivalents to enhance blood flow in order to match tissue oxygen demand (e.g., during hypoxic vasodilation). Newborn babies normally carry two variants of hemoglobin, adult (Hb A) and fetal (Hb F). It is well known that Hb F binds oxygen more tightly than Hb A in order to facilitate oxygen scavenging across the placenta from maternal Hb A. Given that SNO-Hb is preferentially formed on oxygenated Hb, we hypothesized that Hb F may also bind a higher concentration of NO. Previous studies examining the NO content of cord blood were disadvantaged by looking at cord blood Hb as a whole. To date, no attempt has been made to determine the basal levels of NO bound to each Hb variant independently. Therefore, we aimed to separate the variants and measure the NO/SNO content of Hbs F and A in order to establish basal levels for each variant in cord blood at term. We reasoned that the results could improve insight into mechanisms of abnormal perinatal transitions and the selection of therapies involving NO signaling and/or RBC transfusion. Methods Venous and arterial umbilical cord blood samples were collected immediately after normal term cesarean sections of infants with minimum gestational age of 37 weeks. RBC samples were washed in pH 7.4 phosphate buffered saline (PBS) with 100 µM diethylene triamine pentaacetic acid (DTPA) chelator to preserve SNOs, and hypotonically lysed. Total Hb was obtained through purification of the lysate through a Sephadex G-25 column. Partially purified Hb (200 µL) was loaded onto a HiTrap Q HP anionic exchange column (5 mL column volume with 34 µm bead size) and subjected to an increasing ionic strength gradient of 0–0.15M NaCl in pH 8.4 Tris buffer. Spectrophotometric analysis corroborated the complete separation of the variants. Isoelectric focusing on a Perkin Elmer Hemoglobin Resolve gel for 50 minutes at 1500 V and 10–15 °C was used in conjunction with an AFSC Hemopure control to identify the respective variants in each fraction. Each Hb variant was reconcentrated in pH 7.4 PBS with 100 µM DTPA via centrifugation through pre-rinsed 10 kDa MW cutoff centrifugal filters. The SNO/NO content of each variant was analyzed by photolysis-chemiluminescence of paired samples diluted to 100 μM Hb with/without 600 μM HgCl2. For samples with lower Hb concentration, a 6-fold molar excess of HgCl2 was also used. The Hg (mercury) acts to cleave NO bound to thiols (i.e., SNO) and is unreactive towards FeNO complexes; thus the difference in the paired sample peaks indicates the amount of SNO-Hb present in the sample. Results In arterial cord blood samples, the amount of SNO-Hb bound to each variant (i.e., fetal and adult) was found to average ∼5 x 10-4 mol SNO per mol of Hb tetramer (Figure 1A). There was no significant difference between Hb F and Hb A with regards to SNO-Hb. Venous cord blood samples had similar results. The amount of total NO (i.e., SNO-Hb and heme-bound NO) bound to each variant was significantly higher on Hb A in arterial samples. (Figure 1B, *, p value < 0.05 vs. Hb F by paired t-test). Conclusions Both Hb A and Hb F carry substantial and similar amounts of SNO adduct. Given the lower percentage (∼15-20%) of Hb A as a constituent in the total Hb of cord blood at term, the finding of a higher total NO content on Hb A than Hb F suggests that the presence of Hb A may be important to the total NO bioavailability in newborns. Vasodilator NO/SNO is known to be essential in the healthy cardiopulmonary transition to air breathing at birth. Thus, fundamental and translational studies assessing these species in newborns experiencing difficult transitions or pathophysiological states (e.g., persistent pulmonary hypertension of the newborn, PPHN) may provide potential biomarkers with utility in early detection of disease, prognosis, and intervention selection and management. The results and methodology presented here have broad applications to numerous areas of hematology and other medicine including neonatal intensive care, therapeutic induction of HbF in sickle cell disease patients, and decision-making in transfusion medicine. Disclosures: No relevant conflicts of interest to declare.

Erratum to: Determine Multiple Elements Simultaneously in the Sera of Umbilical Cord Blood Samples—a Very Simple Method

2017 ◽  
Vol 177 (1) ◽  
pp. 9-9 ◽  
Author(s):  
Chunmei Liang ◽  
Zhijuan Li ◽  
Xun Xia ◽  
Qunan Wang ◽  
Ruiwen Tao ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Simple Method ◽  
Blood Samples

scholarly journals Iron stores at birth in a full-term normal birth weight birth cohort with a low level of inflammation

2020 ◽  
Vol 40 (12) ◽  
Author(s):  
Joy Y. Zhang ◽  
Jing Wang ◽  
Qinsheng Lu ◽  
Meizhen Tan ◽  
Ru Wei ◽  
...  
Keyword(s):  
Birth Weight ◽  
Cord Blood ◽  
Birth Cohort ◽  
Umbilical Cord ◽  
Serum Ferritin ◽  
Umbilical Cord Blood ◽  
Normal Birth Weight ◽  
Blood Samples ◽  
Iron Stores ◽  
Normal Birth

Abstract Iron stores at birth are essential to meet iron needs during the first 4–6 months of life. The present study aimed to investigate iron stores in normal birth weight, healthy, term neonates. Umbilical cord blood samples were collected from apparently normal singleton vaginal deliveries (n=854). Subjects were screened and excluded if C-reactive protein (CRP) &gt; 5 mg/l or α1-acid glycoprotein (AGP) &gt; 1 g/l, preterm (&lt;37 complete weeks), term &lt; 2500g or term &gt; 4000g. In total, 762 samples were included in the study. Serum ferritin, soluble transferrin receptor (sTfR), hepcidin, and erythropoietin (EPO) were measured in umbilical cord blood samples; total body iron (TBI) (mg/kg) was calculated using sTfR and ferritin concentrations. A total of 19.8% newborns were iron deficient (ferritin 35 μg/l) and an additional 46.6% had insufficient iron stores (ferritin &lt; 76 μg/l). There was a positive association between serum ferritin and sTfR, hepcidin, and EPO. Gestational age was positively associated with ferritin, sTfR, EPO, and hepcidin. In conclusion, we demonstrate a high prevalence of insufficient iron stores in a Chinese birth cohort. The value of cord sTfR and TBI in the assessment of iron status in the newborn is questionable, and reference ranges need to be established.

scholarly journals Investigation on antibody level of umbilical cord blood measles in 2017 in ankang city, Shaanxi province, China

2020 ◽  
Author(s):  
Chunge Wan
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Shaanxi Province ◽  
Service Model ◽  
Childbearing Age ◽  
Igg Antibody ◽  
Blood Samples ◽  
Measles Antibodies ◽  
Positive Rate

Abstract Background Through the examination and analysis of the level of the antibody against the umbilical cord blood measles in maternal newborn in ankang city, to explore prevention and control measures and strategies of measles. Methods Using indirect enzyme-linked immunoadsorption testto test the level of Measles IgG antibody in 848 randomly collected neonatal umbilical cord blood samples,and Using descriptive epidemiological methods,Enter relevant data into Microsoft Excel to establish a database,Statistical analysis using SPSS. Results The positive rate of measles IgG antibody was 84.43 % in 848 neonatal umbilical cord blood samples in Ankang City.There is no statistical difference in the positive rate of neonatal umbilical cord blood measles IgG antibody in different age groups（P>0.05）,However, the positive rate of neonatal umbilical cord blood measles IgG showed a decreasing trend with the increase of maternal age;Maternal and newborn umbilical cord blood measles IgG antibody positive rate and different forms of residence, different maternal births has nothing to do with,but it is related to the county and district where it is located and the vaccination service model.In the counties where the quality of vaccination services is good, and in the areas where the township vaccination service model is implemented, the positive rate of maternal newborn umbilical cord blood measles IgG antibodies is higher, and the difference is statistically significant(P & lt; 0.05). Conclusions Vaccination of measles ingredients before pregnancy in women of childbearing age can not only increase the level of measles antibodies in people of childbearing age, but also increase the level of measles antibodies in infants born in August before the birth of Mazhenyimiao, thus effectively reducing the incidence of measles in children over 15 years of age and within the age of 8 months.It is of great significance to strengthen the standardized administration of vaccination, adjust the vaccination service model, and vigorously promote the centralized vaccination service model in townships and towns to reduce the incidence of measles.

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