Sequence-specific detection method for reverse transcription, loop-mediated isothermal amplification of HIV-1

2009 ◽  
Vol 81 (6) ◽  
pp. 966-972 ◽  
Author(s):  
Kelly A. Curtis ◽  
Donna L. Rudolph ◽  
S. Michele Owen
Keyword(s):  
Reverse Transcription ◽  
Detection Method ◽  
Isothermal Amplification ◽  
Specific Detection ◽  
Loop Mediated Isothermal Amplification ◽  
Hiv 1

scholarly journals Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) on a Chip from Whole Blood

Engineering ◽  
2015 ◽  
Vol 1 (3) ◽  
pp. 324-335 ◽  
Author(s):  
Gregory L. Damhorst ◽  
Carlos Duarte-Guevara ◽  
Weili Chen ◽  
Tanmay Ghonge ◽  
Brian T. Cunningham ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Whole Blood ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Hiv 1

scholarly journals Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2

2020 ◽  
Author(s):  
Gun-Soo Park ◽  
Keunbon Ku ◽  
Seung-Hwa Baek ◽  
Seong Jun Kim ◽  
Seung Il Kim ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Detection Method ◽  
Colorimetric Detection ◽  
Lamp Assay ◽  
Cross Reactivity ◽  
Isothermal Amplification ◽  
Genomic Rna ◽  
Loop Mediated Isothermal Amplification ◽  
Human Coronaviruses ◽  
High Level

AbstractEpidemics of Coronavirus Disease 2019 (COVID-19) now have more than 100,000 confirmed cases worldwide. Diagnosis of COVID-19 is currently performed by RT-qPCR methods, but the capacity of RT-qPCR methods is limited by its requirement of high-level facilities and instruments. Here, we developed and evaluated RT-LAMP assays to detect genomic RNA of SARS-CoV-2, the causative virus of COVID-19. RT-LAMP assays in this study can detect as low as 100 copies of SARS-CoV-2 RNA. Cross-reactivity of RT-LAMP assays to other human Coronaviruses was not observed. We also adapted a colorimetric detection method for our RT-LAMP assay so that the tests potentially performed in higher throughput.

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