Molecular epidemiology of human adenovirus isolated from children hospitalized with acute respiratory infection in São Paulo, Brazil

2006 ◽  
Vol 79 (2) ◽  
pp. 174-181 ◽  
Author(s):  
Patrícia O. Moura ◽  
Adriana F. Roberto ◽  
Noely Hein ◽  
Evandro Baldacci ◽  
Sandra E. Vieira ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Respiratory Infection ◽  
Acute Respiratory Infection ◽  
Human Adenovirus ◽  
Sao Paulo ◽  
São Paulo

scholarly journals Human bocavirus in hospitalized children under 5 years with acute respiratory infection, São Paulo, Brazil, 2010

2018 ◽  
Vol 163 (5) ◽  
pp. 1325-1330 ◽  
Author(s):  
Patricia Evelin Silva ◽  
Cristina Adelaide Figueiredo ◽  
Adriana Luchs ◽  
Terezinha Maria de Paiva ◽  
Margarete Aparecida Benega Pinho ◽  
...  
Keyword(s):  
Respiratory Infection ◽  
Acute Respiratory Infection ◽  
Sao Paulo ◽  
Human Bocavirus ◽  
Hospitalized Children ◽  
São Paulo ◽  
Children Under 5

Discrimination of adenovirus types circulating in urban sewage and surface polluted waters in São Paulo city, Brazil

2004 ◽  
Vol 4 (2) ◽  
pp. 79-85 ◽  
Author(s):  
F.M. Santos ◽  
M.J. Vieira ◽  
P. Garrafa ◽  
T.A. Monezi ◽  
V.H. Pellizari ◽  
...  
Keyword(s):  
Human Adenovirus ◽  
Sao Paulo ◽  
São Paulo ◽  
Hexon Gene ◽  
Risk Of Infection ◽  
Urban Sewage ◽  
Pcr Product ◽  
Step Procedure ◽  
Surrounding Areas ◽  
Polluted Waters

Owing to frequent overflow of sewage-polluted creeks and rivers in São Paulo city, the population that lives in those surrounding areas is constantly exposed to a high risk of infection by many pathogens. Our aim was to evaluate the occurrence of human adenovirus, especially F species (HAdV-40 and HAdV-41), in sewage and creek water in São Paulo city. A total of 95 water samples were collected from July 1998 to June 1999 and from September 2000 to December 2001 at Sewage Pumping Station Edu Chaves and Creek Pirajussara. Four-litre samples were concentrated by a two-step procedure based on filtration through electropositive filter membrane and ultracentrifugation. Adenoviruses were detected in 69 samples (72.6%) after amplification of a sequence of the hexon gene by PCR. The discrimination of human adenovirus F was done by RFLP. A first screening was performed by restriction of PCR product with TaqI, followed by the discrimination of HAdV-40 and HAdV-41 by RsaI restriction. Sixty-two (89.8%) samples showed F species viruses. Among them, human adenovirus, serotype 41, was the most frequently detected in 17 (62.9%) out of 27 samples and serotype 40 was identified only in 7 (25.9%) samples. No seasonal distribution of adenoviruses was observed.

scholarly journals An Outbreak of Acute Respiratory Infection at a Training Base in Beijing, China Due to Human Adenovirus Type B55

2020 ◽  
Author(s):  
Guilan Lu ◽  
Xiaomin Peng ◽  
Renqing Li ◽  
Yimeng Liu ◽  
Zhanguo Wu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Real Time ◽  
Respiratory Infection ◽  
Real Time Pcr ◽  
Acute Respiratory Infection ◽  
Index Case ◽  
Human Adenovirus ◽  
Whole Genome ◽  
Pharyngeal Swab ◽  
Tract Infections

Abstract Background: Twelve students experienced symptoms of acute respiratory infection (ARI) at a training base in Beijing from August 26 to August 30, 2015. We investigated the cause of this ARI outbreak. Methods: In partnership with the local center for disease control, we collected a total of twelve pharyngeal swab specimens as well as demographic information for the affected patients. We used multiplex real-time PCR to screen for sixteen common respiratory viruses in these samples. To isolate HAdV, we inoculated Hep-2 cells with the human adenovirus (HAdV)-positive samples and then carried out sequencing and phylogenetic analysis of the hexon, fiber, and penton genes of the isolated adenoviruses. In addition, we analyzed the entire genome of one strain isolated from the index case to identify single-nucleotide substitutions. Results: We identified ten HAdV-positive students using multiplex real-time PCR. None of the students were co-infected with other viruses. We successfully isolated seven HAdV strains from the pharyngeal swab specimens. The coding sequences of the hexon, fiber, and penton genes of these seven HAdV strains were identical, suggesting that they represented seven strains from a single virus clone. One HAdV isolate obtained from the index case, BJDX-01-2015, was selected for whole genome analysis. From this isolate, we obtained a 34,774-nucleotide sequence. The genome of BJDX-01-2015 clustered with HAdV-B55 in phylogenetic analyses and had 99.97% identity with human adenovirus 55 isolate HAdV-B/CHN/BJ01/2011/55 (GenBank accession no. JX491639). Conclusions: We identified HAdV-B55 as the strain associated with the August 2015 ARI outbreak at a training base in Beijing. This was the first reported outbreak in Beijing due to HAdV-B55. Continuous surveillance of respiratory adenoviruses is urgently needed to understand the epidemiological and evolutionary features of HAdV-B55, and an epidemiological modeling approach may provide further insights into this emerging public health threat. Furthermore, the clinical laboratory data from this outbreak provides important reference for the clinical diagnosis and may ultimately aid in informing the development of strategies to control and prevent respiratory tract infections caused by HAdV-B55. Keywords: Outbreak, Human adenovirus, Acute Respiratory Infection, Phylogenetic Analysis, Whole Genome Sequencing

Oxacillin- and Quinolone-Resistant Staphylococcus aureus in Sao Paulo, Brazil: A Multicenter Molecular Epidemiology Study

1993 ◽  
Vol 14 (5) ◽  
pp. 260-264 ◽  
Author(s):  
Helio S. Sader ◽  
Antonio C. Pignatari ◽  
Richard J. Hollis ◽  
Ivani Leme ◽  
Ronald N. Jones
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Epidemiology ◽  
Sao Paulo ◽  
São Paulo ◽  
Epidemiology Study

scholarly journals Molecular epidemiology of rubella viruses involved in congenital rubella infections in São Paulo, Brazil, between 1996 and 2009

2013 ◽  
Vol 85 (11) ◽  
pp. 2034-2041 ◽  
Author(s):  
Suely Pires Curti ◽  
Cristina Adelaide Figueiredo ◽  
Maria Isabel de Oliveira ◽  
Joelma Queiroz Andrade ◽  
Marcelo Zugaib ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Sao Paulo ◽  
São Paulo ◽  
Congenital Rubella

Molecular Epidemiology of Human Parechoviruses in Children With Acute Respiratory Infection in Spain

2013 ◽  
Vol 32 (7) ◽  
pp. 802-803 ◽  
Author(s):  
María Cabrerizo ◽  
Cristina Calvo ◽  
Gloria Trallero ◽  
María Luz García-García ◽  
María Arroyas ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Respiratory Infection ◽  
Acute Respiratory Infection

scholarly journals MOLECULAR EPIDEMIOLOGY OF A MEASLES VIRUS IN SAO PAULO, BRAZIL: AN IMPORTED CASE

2013 ◽  
Vol 55 (4) ◽  
pp. 292-292
Author(s):  
Maria Isabel de Oliveira ◽  
Cristina Adelaide Figueiredo ◽  
Ana Maria Sardinha Afonso ◽  
Marilda Mendonca Siqueira ◽  
Xenia Rawena Medeiros Romeu Lemos ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Measles Virus ◽  
Sao Paulo ◽  
São Paulo ◽  
Imported Case
Sign in / Sign up

Export Citation Format

Share Document