Quantification of Epstein-Barr virus load in peripheral blood of human immunodeficiency virus-infected patients using real-time PCR

2001 ◽  
Vol 65 (3) ◽  
pp. 543-552 ◽  
Author(s):  
Axelle Dehee ◽  
Catherine Asselot ◽  
Tristan Piolot ◽  
Christine Jacomet ◽  
Willy Rozenbaum ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Epstein Barr Virus ◽  
Virus Load ◽  
Barr Virus ◽  
Immunodeficiency Virus ◽  
Epstein Barr

Quantitative Analysis of Epstein-Barr Virus Load by Using a Real-Time PCR Assay

1999 ◽  
Vol 37 (1) ◽  
pp. 132-136 ◽  
Author(s):  
Hiroshi Kimura ◽  
Makoto Morita ◽  
Yumi Yabuta ◽  
Kiyotaka Kuzushima ◽  
Koji Kato ◽  
...  
Keyword(s):  
Real Time ◽  
Infectious Mononucleosis ◽  
Real Time Pcr ◽  
Copy Number ◽  
Epstein Barr Virus ◽  
Pcr Assay ◽  
Virus Load ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could detect from 2 to over 107 copies of EBV DNA with a wide linear range. We estimated the virus load in peripheral blood mononuclear cells (PBMNC) from patients with symptomatic EBV infections. The mean EBV-DNA copy number in the PBMNC was 103.7 copies/μg of DNA in patients with EBV-related lymphoproliferative disorders, 104.1 copies/μg of DNA in patients with chronic active EBV infections, and 102.2copies/μg of DNA in patients with infectious mononucleosis. These numbers were significantly larger than those in either posttransplant patients or immunocompetent control patients without EBV-related diseases. In a patient with infectious mononucleosis, the virus load decreased as the symptoms resolved. The copy number of EBV DNA in PBMNC from symptomatic EBV infections was correlated with the EBV-positive cell number determined by the in situ hybridization assay (r = 0.842; P < 0.0001). These results indicate that the real-time PCR assay is useful for diagnosing symptomatic EBV infection and for monitoring the virus load.

Impact of Epstein-Barr virus load, virus genotype, and frequency of the 30 bp deletion in the viral BNLF-1 gene in patients harboring the human immunodeficiency virus

2013 ◽  
Vol 85 (12) ◽  
pp. 2110-2118 ◽  
Author(s):  
Leila Bertoni Giron ◽  
Suzane Ramos da Silva ◽  
Alexandre Naime Barbosa ◽  
Ricardo Augusto Monteiro de Barros Almeida ◽  
Lenice do Rosário de Souza ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Epstein Barr Virus ◽  
Virus Genotype ◽  
Virus Load ◽  
Barr Virus ◽  
Immunodeficiency Virus ◽  
Epstein Barr

scholarly journals Plasma Epstein-Barr Virus Load as an Early Biomarker and Prognostic Factor of Human Immunodeficiency Virus–related Lymphomas

2018 ◽  
Vol 68 (5) ◽  
pp. 834-843 ◽  
Author(s):  
Josep Muncunill ◽  
Maria-Joao Baptista ◽  
Águeda Hernandez-Rodríguez ◽  
Judith Dalmau ◽  
Olga Garcia ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Prognostic Factor ◽  
Epstein Barr Virus ◽  
Virus Load ◽  
Barr Virus ◽  
Immunodeficiency Virus ◽  
Early Biomarker ◽  
Epstein Barr

scholarly journals Epstein-Barr Virus Load in Children Infected With Human Immunodeficiency Virus Type 1 in Uganda

2014 ◽  
Vol 210 (3) ◽  
pp. 392-399 ◽  
Author(s):  
M. R. Petrara ◽  
M. Penazzato ◽  
W. Massavon ◽  
S. Nabachwa ◽  
M. Nannyonga ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Epstein Barr Virus ◽  
Virus Load ◽  
Barr Virus ◽  
Immunodeficiency Virus ◽  
Epstein Barr

Quantification of Epstein-Barr virus load in Argentinean transplant recipients using real-time PCR

2004 ◽  
Vol 31 (1) ◽  
pp. 58-65 ◽  
Author(s):  
Judith Yancoski ◽  
Silvia Danielian ◽  
Juan Ibañez ◽  
Amalia Turconi ◽  
Miriam Cuarterolo ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Transplant Recipients ◽  
Virus Load ◽  
Barr Virus ◽  
Epstein Barr

Epstein-Barr Virus load and immune activation in Human Immunodeficiency Virus type 1-infected patients

2012 ◽  
Vol 53 (3) ◽  
pp. 195-200 ◽  
Author(s):  
Maria Raffaella Petrara ◽  
Anna Maria Cattelan ◽  
Marisa Zanchetta ◽  
Lolita Sasset ◽  
Riccardo Freguja ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immune Activation ◽  
Epstein Barr Virus ◽  
Virus Load ◽  
Barr Virus ◽  
Immunodeficiency Virus ◽  
Epstein Barr

Characteristics of Epstein-Barr Virus-Associated Hemophagocytic Lymphohistiocytosis (HLH) in European Children.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3883-3883
Author(s):  
Hans-Joachim S. Wagner ◽  
Katrin Beutel ◽  
Marion E. Schneider ◽  
Gritta E. Janka
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Hemophagocytic Lymphohistiocytosis ◽  
Epstein Barr Virus ◽  
Blood Analysis ◽  
Pcr Analysis ◽  
Course Of Disease ◽  
Barr Virus ◽  
Epstein Barr

Abstract Hemophagocytic lymphohistiocytosis (HLH) represents a severe sepsis-like disease with massive hypercytokinemia and with proliferating and organ-infiltrating phagocytes leading to cytopenia of different hematopoietic lineages. Besides primary familial cases of HLH, infections can cause secondary forms of HLH, among which Epstein-Barr virus (EBV) infections are the most frequent trigger. Between 2003 and 2005, 10 cases of EBV-associated HLH were analysed at a local subcenter of the international HLH study group. Serial blood samples from these 4 boys and 6 girls (age range: 2–14 years, median of age: 4 years) were examined for EBV load in peripheral blood mononuclear cells (PBMC) and plasma by real-time PCR analysis. EBV loads in both PBMC and plasma were significantly elevated in all HLH patients as compared with healthy controls and also with immunocompetent patients suffering from primary EBV infections. In one patient with excessively elevated EBV load in peripheral blood, analysis of EBV-DNA in separated T and B cells by real-time PCR showed an EBV infection of both cell types. During the course of disease, EBV load in HLH patients reflected the activity of disease. Regarding the clinical course and biochemical markers of disease such as LDH, ferritin or soluble IL2-receptor levels in plasma, patients with EBV-associated HLH did not differ from other patients with HLH. Conclusion: Measurement of EBV-load by real-time PCR facilitates detection of EBV-associated cases of HLH and monitoring of affected patients during the course of disease. EBV load analysis should become part of the initial routine examinations of newly diagnosed HLH patients in order to identify the frequency of EBV-associated forms of HLH. Without molecular diagnosis by means of EBV load measurement in peripheral blood, EBV-associated cases of HLH may be underdiagnosed in non-Asian populations.

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