Quantitation of cytomegalovirus DNA by real-time polymerase chain reaction in peripheral blood specimens of patients with solid organ transplants: Comparison with end-point PCR and pp65 antigen test

2006 ◽  
Vol 78 (7) ◽  
pp. 915-922 ◽  
Author(s):  
Tiziano Allice ◽  
Marco Enrietto ◽  
Fabrizia Pittaluga ◽  
Silvia Varetto ◽  
Alessandro Franchello ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Peripheral Blood ◽  
Solid Organ ◽  
Organ Transplants ◽  
Antigen Test ◽  
Chain Reaction ◽  
Pp65 Antigen ◽  
Polymerase Chain ◽  
Blood Specimens

scholarly journals Expression of DROSHA and DICER genes in peripheral blood leukocytes in lung sarcoidosis

2018 ◽  
Vol 90 (3) ◽  
pp. 21-24
Author(s):  
I E Malysheva ◽  
O V Balan ◽  
E L Tikhonovich ◽  
T O Volkova
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Peripheral Blood ◽  
Messenger Rna ◽  
Peripheral Blood Leukocytes ◽  
Chain Reaction ◽  
Blood Leukocytes ◽  
Healthy Donors ◽  
Polymerase Chain

Aim. To study the expression level of the genes DROSHA and DICER in peripheral blood leukocytes (PBL) of patients with sarcoidosis of the lungs Materials and methods. The study included 32 patients diagnosed with persistent lung sarcoidosis (mean age 41.56±1.27 years) and 36 healthy donors (control; mean age 42.79±1.95 years). The level of expression of messenger RNA (mRNA) of the genes DROSHA and DICER were determined in PBL of healthy donors and patients with sarcoidosis of the lung by polymerase chain reaction in real time. Results. As a result of the conducted researches it is established that the level of drosha gene expression in PBL patients with sarcoidosis of lungs is significantly reduced in comparison with the control (p

scholarly journals Monitoring of cytomegalovirus (CMV) infection in solid organ transplant recipients: quantitation of CMV DNAemia by two real-time polymerase chain reaction assays

2016 ◽  
Vol 31 (3) ◽  
Author(s):  
Angela Chiereghin ◽  
Giulia Piccirilli ◽  
Gabriele Turello ◽  
Diego Squarzoni ◽  
Claudia Pavia ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Quantitative Result ◽  
Lower Amount ◽  
Solid Organ ◽  
Transplant Recipients ◽  
Chain Reaction ◽  
Clinical Value ◽  
Polymerase Chain ◽  
Cmv Dnaemia

<em>Background and aim:</em> Quantification of cytomegalovirus (CMV) DNAemia is essential in clinical management of post-transplant infection. We evaluated the performances of two quantitative real-time polymerase chain reaction (PCR) assays. <br /><em>Materials and Methods</em>: 114 serial whole blood samples collected from 14 actively infected transplant recipients were processed by Abbott RealTime CMV PCR kit (Abbott Molecular) and CMV ELITe MGB™ kit (ELITech Group). The Quality Control for Molecular Diagnostics human CMV panels was also tested. <br /><em>Results</em>: Sixteen (14%) samples resulted negative and 59 (51.7%) positive with a quantitative result for both assays. In the 59 samples, the coefficient of correlation was 0.856. Bland-Altman analysis showed a mean difference of &lt;0.11 log10 copies/mL (standard deviation=0.38 log10 copies/mL). The assays gave CMV-DNA loads differing by 1 log10 DNA copies/mL in 57 samples (96.6%) and by &lt;0.5 log10 DNA copies/mL in 48 samples (81.3%). Eleven (9.6%) samples were positive with a quantitative result with Abbott and negative with ELITech. Sixteen (14%) positive samples with a quantitative result for Abbott resulted positive but below the lower limit of quantification (LLQ) for ELITech. Twelve (10.5%) samples resulted negative with ELITech and positive but below the LLQ with Abbott. No samples were positive with ELITech and negative with Abbott. <br /><em>Conclusions</em>: The assays showed a good correlation between CMVDNA levels detected and variation in CMV-DNA &lt;0.5 log10 was observed in the majority of the samples. The viral load kinetic profiles of the assays were overlapping in all patients, but Abbott showed higher sensitivity in samples containing lower amount of DNA. The clinical value of this greater sensitivity requires further investigation.

Epstein-Barr virus load in the peripheral blood of patients with rheumatoid arthritis: Accurate quantification using real-time polymerase chain reaction

2003 ◽  
Vol 48 (5) ◽  
pp. 1223-1228 ◽  
Author(s):  
Nathalie Balandraud ◽  
Jean Baptiste Meynard ◽  
Isabelle Auger ◽  
Helene Sovran ◽  
Benedicte Mugnier ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Peripheral Blood ◽  
Epstein Barr Virus ◽  
Chain Reaction ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr ◽  
Accurate Quantification

scholarly journals Prognostic Significance of the Detection of Peripheral Blood CEACAM5mRNA-Positive Cells by Real-Time Polymerase Chain Reaction in Operable Colorectal Cancer

2010 ◽  
Vol 17 (1) ◽  
pp. 165-173 ◽  
Author(s):  
Nikolaos Vardakis ◽  
Ippokratis Messaritakis ◽  
Chara Papadaki ◽  
Georgios Agoglossakis ◽  
Maria Sfakianaki ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Peripheral Blood ◽  
Prognostic Significance ◽  
Chain Reaction ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document