DNA microarray technique for detection and identification of seven flaviviruses pathogenic for man

2005 ◽  
Vol 77 (4) ◽  
pp. 528-540 ◽  
Author(s):  
Henrik Nordström ◽  
Kerstin I. Falk ◽  
Gunnel Lindegren ◽  
Mehrdad Mouzavi-Jazi ◽  
Annelie Waldén ◽  
...  
Keyword(s):  
Dna Microarray ◽  
Detection And Identification ◽  
Microarray Technique

An electronic DNA microarray technique for detection and differentiation of viable Campylobacter species

The Analyst ◽  
2006 ◽  
Vol 131 (8) ◽  
pp. 907 ◽  
Author(s):  
Hai Zhang ◽  
Zhilong Gong ◽  
Odell Pui ◽  
Yanming Liu ◽  
Xing-Fang Li
Keyword(s):  
Dna Microarray ◽  
Campylobacter Species ◽  
Microarray Technique

scholarly journals Detection and Identification of Mycobacterium Species Isolates by DNA Microarray

2003 ◽  
Vol 41 (6) ◽  
pp. 2605-2615 ◽  
Author(s):  
M. Fukushima ◽  
K. Kakinuma ◽  
H. Hayashi ◽  
H. Nagai ◽  
K. Ito ◽  
...  
Keyword(s):  
Dna Microarray ◽  
Mycobacterium Species ◽  
Detection And Identification

scholarly journals Whole-Genome Analysis of Genes Regulated by the Bacillus subtilis Competence Transcription Factor ComK

2002 ◽  
Vol 184 (9) ◽  
pp. 2344-2351 ◽  
Author(s):  
Mitsuo Ogura ◽  
Hirotake Yamaguchi ◽  
Kazuo Kobayashi ◽  
Naotake Ogasawara ◽  
Yasutaro Fujita ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Bacillus Subtilis ◽  
Genetic Transformation ◽  
Dna Microarray ◽  
Genome Analysis ◽  
Transformation Efficiency ◽  
The Other ◽  
Whole Genome ◽  
Whole Genome Analysis ◽  
Microarray Technique

ABSTRACT The Bacillus subtilis competence transcription factor ComK is required for establishment of competence for genetic transformation. In an attempt to study the ComK factor further, we explored the genes regulated by ComK using the DNA microarray technique. In addition to the genes known to be dependent on ComK for expression, we found many genes or operons whose ComK dependence was not known previously. Among these genes, we confirmed the ComK dependence of 16 genes by using lacZ fusions, and three genes were partially dependent on ComK. Transformation efficiency was significantly reduced in an smf disruption mutant, although disruption of the other ComK-dependent genes did not result in significant decreases in transformation efficiency. Nucleotide sequences similar to that of the ComK box were found for most of the newly discovered genes regulated by ComK.

scholarly journals Development of a DNA Microarray Method for Detection and Identification of All 15 Distinct O-Antigen Forms of Legionella pneumophila

2013 ◽  
Vol 79 (21) ◽  
pp. 6647-6654 ◽  
Author(s):  
Boyang Cao ◽  
Fangfang Yao ◽  
Xiangqian Liu ◽  
Lu Feng ◽  
Lei Wang
Keyword(s):  
Dna Microarray ◽  
Legionella Pneumophila ◽  
Detection Sensitivity ◽  
Immunological Methods ◽  
Clinical Samples ◽  
Content Type ◽  
O Antigen ◽  
Detection And Identification ◽  
Legionnaires Disease ◽  
Reference Strains

ABSTRACTLegionellais ubiquitous in many environments. At least 50 species and 70 serogroups of the Gram-negative bacterium have been identified. Of the 50 species, 20 are pathogenic, andLegionella pneumophilais responsible for the great majority (approximately 90%) of the Legionnaires' disease cases that occur. Furthermore, of the 15L. pneumophilaserogroups identified, O1 alone causes more than 84% of the Legionnaires' disease cases that occur worldwide. Rapid and reliable assays for the detection and identification ofL. pneumophilain water, environmental, and clinical samples are in great demand.L. pneumophilabacteria are traditionally identified by their O antigens by immunological methods. We have recently developed an O serogroup-specific DNA microarray for the detection of all 15 distinct O-antigen forms ofL. pneumophila, including serogroups O1 to O15. A total of 35 strains were used to verify the specificity of the microarray, including 15L. pneumophilaO-antigen standard reference strains and sevenL. pneumophilaclinical isolates as target strains, seven reference strains of other non-pneumophila Legionellaspecies as closely related strains, and six non-Legionellabacterial species as nonrelated strains. The detection sensitivity was 1 ng of genomic DNA or 0.4 CFU/ml in water samples with filter enrichment and plate culturing. This study demonstrated that the microarray allows specific, sensitive, and reproducible detection ofL. pneumophilaserogroups. To the best of our knowledge, this is the first report of a microarray serotyping method for all 15 distinct O-antigen forms ofL. pneumophila.

scholarly journals The Hydrogenase Chip: a tiling oligonucleotide DNA microarray technique for characterizing hydrogen-producing and -consuming microbes in microbial communities

2011 ◽  
Vol 6 (4) ◽  
pp. 814-826 ◽  
Author(s):  
Ian PG Marshall ◽  
Dusty RV Berggren ◽  
Mohammad F Azizian ◽  
Luke C Burow ◽  
Lewis Semprini ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Dna Microarray ◽  
Microarray Technique

A microbial diagnostic microarray technique for the sensitive detection and identification of pathogenic bacteria in a background of nonpathogens

2007 ◽  
Vol 360 (2) ◽  
pp. 244-254 ◽  
Author(s):  
Tanja Kostić ◽  
Alexandra Weilharter ◽  
Salvatore Rubino ◽  
Giuseppe Delogu ◽  
Sergio Uzzau ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Sensitive Detection ◽  
Detection And Identification ◽  
Microarray Technique
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