Semiquantitative PCR analysis of Epstein-Barr virus DNA in clinical samples of patients with EBV-associated diseases

2001 ◽  
Vol 65 (2) ◽  
pp. 348-357 ◽  
Author(s):  
Astrid Meerbach ◽  
Bernd Gruhn ◽  
Renate Egerer ◽  
Udo Reischl ◽  
Felix Zintl ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Clinical Samples ◽  
Pcr Analysis ◽  
Barr Virus ◽  
Associated Diseases ◽  
Epstein Barr

scholarly journals Elevated Serum Transforming Growth Factor β1 Levels in Epstein-Barr Virus-Associated Diseases and Their Correlation with Virus-Specific Immunoglobulin A (IgA) and IgM

2000 ◽  
Vol 74 (5) ◽  
pp. 2443-2446 ◽  
Author(s):  
Jingwu Xu ◽  
Ali Ahmad ◽  
James F. Jones ◽  
Riccardo Dolcetti ◽  
Emanuela Vaccher ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epstein Barr Virus ◽  
Transforming Growth Factor ◽  
Immunoglobulin A ◽  
Elevated Serum ◽  
Transforming Growth Factor Β ◽  
Serum Levels ◽  
Barr Virus ◽  
Associated Diseases ◽  
Epstein Barr

ABSTRACT Transforming growth factor β (TGF-β) is an immunosuppressive cytokine which can induce immunoglobulin A (IgA) switch and Epstein-Barr virus (EBV) replication in latently infected cells. Here we report elevated serum levels of TGF-β in various EBV-associated diseases correlating positively with EBV-specific IgA titers and negatively with IgM titers, suggesting a role for this cytokine in the pathogenesis of these diseases.

Modulation of Caspase-8 and FLICE-Inhibitory Protein Expression as a Potential Mechanism of Epstein-Barr Virus Tumorigenesis in Burkitt’s Lymphoma

Blood ◽  
1999 ◽  
Vol 94 (5) ◽  
pp. 1727-1737 ◽  
Author(s):  
Clifford G. Tepper ◽  
Michael F. Seldin
Keyword(s):  
Cell Lines ◽  
Epstein Barr Virus ◽  
Burkitt’S Lymphoma ◽  
Burkitt's Lymphoma ◽  
Pcr Analysis ◽  
Caspase 8 ◽  
Rt Pcr ◽  
Inhibitory Protein ◽  
Barr Virus ◽  
Epstein Barr

Abstract Ligation of the Fas receptor induces death-inducing signaling complex (DISC) formation, caspase activation, and subsequent apoptotic death of several cell types. Epstein-Barr virus (EBV)-positive group III Burkitt’s lymphoma (BL) cell lines have a marked resistance to Fas-mediated apoptosis, although expressing each of the DISC components, Fas/ APO-1–associated death domain protein (FADD), and caspase-8 (FLICE/MACH/Mch5). The apoptotic pathway distal to the DISC is intact because ceramide analogs, staurosporine, and granzyme B activate caspase-3 and induce apoptosis. Fas resistance was not explained by the putative death-attenuating caspase-8 isoforms. However, while Fas-activated cytosolic extracts from sensitive cells were capable of processing both procaspase-8 and procaspase-3 into active subunit forms, resistant cell extracts did not possess either of these activities. Accordingly, reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed higher transcript levels for the FLICE-inhibitory protein (FLIPL) in resistant cells and the ratio of caspase-8 to FLIPLmeasured by competition RT-PCR analysis directly correlated with susceptibility to Fas-mediated apoptosis of all cell lines. In addition, modification of the caspase-8/FLIPL ratio by caspase-8 or FLIPL overexpression was able to alter the susceptibility status of the cell lines tested. Our results imply that the relative levels of caspase-8 and FLIPL are an important determinant of susceptibility to Fas-mediated apoptosis.

scholarly journals Corrigendum: Role of Viral and Host microRNAs in Immune Regulation of Epstein-Barr Virus-Associated Diseases

2020 ◽  
Vol 11 ◽  
Author(s):  
Hisashi Iizasa ◽  
Hyoji Kim ◽  
Andy Visi Kartika ◽  
Yuichi Kanehiro ◽  
Hironori Yoshiyama
Keyword(s):  
Immune Regulation ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Associated Diseases ◽  
Epstein Barr

Prophylactic and therapeutic strategies for Epstein–Barr virus-associated diseases: emerging strategies for clinical development

2019 ◽  
Vol 18 (5) ◽  
pp. 457-474 ◽  
Author(s):  
Vijayendra Dasari ◽  
Debottam Sinha ◽  
Michelle A. Neller ◽  
Corey Smith ◽  
Rajiv Khanna
Keyword(s):  
Epstein Barr Virus ◽  
Therapeutic Strategies ◽  
Clinical Development ◽  
Barr Virus ◽  
Associated Diseases ◽  
Epstein Barr

scholarly journals Association Between Environmental Factors and Oral Epstein-Barr Virus DNA Loads: A Multicenter Cross-sectional Study in China

2018 ◽  
Vol 219 (3) ◽  
pp. 400-409 ◽  
Author(s):  
Yong-Qiao He ◽  
Xiao-Yu Liao ◽  
Wen-Qiong Xue ◽  
Ya-Fei Xu ◽  
Feng-Hua Xu ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Odds Ratio ◽  
Epstein Barr Virus ◽  
Southern China ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Barr Virus ◽  
Associated Diseases ◽  
Epstein Barr

Abstract Background Oral Epstein-Barr virus (EBV) status reflects host EBV activity and potentially links to EBV-associated diseases, however, factors influencing oral EBV loads or reactivation, such as environmental exposures or host factors, are not fully understood. Methods A 2-stage, multicenter, cross-sectional study of 6558 subjects from 21 administrative cities of southern China and 3 populations from representative geographical areas in China (referred to as the south, north, and northeastern populations) was performed. The relationships between demographical factors and environmental exposures to EBV loads were analyzed by logistic regression models. Results Current smoking, with a dose-response effect, was found to be strongly associated with higher oral EBV loads in the pooled data, with an odds ratio of 1.58 (95% confidence interval, 1.39–1.79), as well as in each of the separate populations. The odds ratio increased to 3.06 when current smokers in southern China were compared to never smokers in northern China. Additionally, higher oral EBV loads tended to be detected in older participants, male participants, and participants in southern China. Conclusions This study provided evidence linking the effect of host-environmental factors, particularly smoking, to oral EBV activity. It could strengthen our understanding of the possible causal roles of EBV-related diseases, which may help to prevent or mitigate EBV-associated diseases.

scholarly journals Methylation analysis of plasma DNA informs etiologies of Epstein-Barr virus-associated diseases

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
W. K. Jacky Lam ◽  
Peiyong Jiang ◽  
K. C. Allen Chan ◽  
Wenlei Peng ◽  
Huimin Shang ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Plasma Dna ◽  
Methylation Analysis ◽  
Barr Virus ◽  
Associated Diseases ◽  
Epstein Barr
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