Detection of enterovirus in human skeletal muscle from patients with chronic inflammatory muscle disease or fibromyalgia and healthy subjects

2003 ◽  
Vol 71 (4) ◽  
pp. 540-547 ◽  
Author(s):  
Fatima Douche-Aourik ◽  
Willy Berlier ◽  
Léonard Féasson ◽  
Thomas Bourlet ◽  
Rafik Harrath ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Healthy Subjects ◽  
Human Skeletal Muscle ◽  
Muscle Disease ◽  
Inflammatory Muscle Disease

Measurement of protein synthesis in human skeletal muscle: further investigation of the flooding technique

1991 ◽  
Vol 81 (s25) ◽  
pp. 557-564 ◽  
Author(s):  
M. A. McNurlan ◽  
P. Essen ◽  
S. D. Heys ◽  
V. Buchan ◽  
P. J. Garlick ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Continuous Infusion ◽  
Healthy Subjects ◽  
Human Skeletal Muscle ◽  
Quadriceps Muscle ◽  
Mean Difference ◽  
Good Agreement ◽  
Stimulation Of

1. The rate of protein synthesis in quadriceps muscle of healthy subjects estimated from the incorporation of l-[1-13C]leucine given by continuous infusion was 1.1%/day. The estimate of protein synthesis from the incorporation of a flooding amount of labelled leucine was 1.8%/day (sd 0.65). The possibility that the higher rate obtained with the flooding technique arose from stimulation of protein synthesis by the large amount of leucine is unlikely. 2. The same rate of protein synthesis (1.7%/day, sd 0.3) was obtained with a flooding amount (0.05 g/kg) of a different amino acid, l-[1-13C]phenylalanine, as was obtained with leucine. 3. Incorporation of l-[1-13C]phenylalanine was not affected by simultaneous injection of leucine (1.7%/day, sd 0.7) or valine (1.6%/day, sd 0.4). 4. Protein synthesis, assessed in a completely different way from the proportion of polyribosomes isolated from the skeletal muscle, was unaltered by the injection of 0.05 g of l-leucine/kg (44.6%, sd 8.5 versus 43.8%, sd 7.7). 5. Good agreement in estimates of protein synthesis was observed in subjects in whom both legs were measured with both l-[1-13C]leucine (mean difference 0.16%/day) and l-[1-13C]phenylalanine (mean difference 0.2%/day).

Cardiac troponin-I is not expressed in fetal and healthy or diseased adult human skeletal muscle tissue

1995 ◽  
Vol 41 (12) ◽  
pp. 1710-1715 ◽  
Author(s):  
G S Bodor ◽  
D Porterfield ◽  
E M Voss ◽  
S Smith ◽  
F S Apple
Keyword(s):  
Skeletal Muscle ◽  
Cardiac Troponin ◽  
Human Skeletal Muscle ◽  
Troponin I ◽  
Tissue Bank ◽  
Cardiac Troponin I ◽  
Muscle Disease ◽  
Adult Skeletal Muscle ◽  
Muscle Sample ◽  
Creatine Kinase Mb

Abstract Cardiac troponin-I (cTnI) is not found in sera of patients with skeletal muscle disease in the absence of myocardial injury. It is not known, however, whether trace amounts of cTnI are expressed in regenerating human skeletal muscle, as has been observed with creatine kinase MB. Using immunohistochemical and biochemical techniques, we investigated cTnI expression in various human muscle tissues: human heart tissue (n = 5), normal adult skeletal muscle (n = 3), and fetal heart (n = 3) and skeletal muscle (n = 3) obtained, respectively, during heart transplant, from autopsy, or from a tissue bank. Specimens from diagnostic tissue biopsies were used as diseased skeletal muscle: polymyositis (PM), n = 13; Duchenne muscular dystrophy (DMD), n = 6. Frozen sections 8 microns thick were stained immunohistochemically for either cTnI or TnI (cardiac or skeletal) by using monoclonal antibodies (MAb) 2B1.9 (cTnI specific) or 3C5.10 (reactive with all TnI isoforms), respectively. cTnI was measured in tissue homogenates by an immunofluorometric assay. Cardiac muscle was stained by both MAbs. Normal fetal and adult skeletal muscle, and samples from all of the PM and DMD patients, stained only with the nonspecific MAb (3C5.10), confirming the sole presence of skeletal TnI. No cTnI was detectable by immunoassay in any skeletal muscle sample. We conclude that cTnI is not expressed in human skeletal muscle during development or during regenerative muscle disease processes such as PM or DMD.

scholarly journals Exercise mimetics and JAK inhibition attenuate IFN-γ–induced wasting in engineered human skeletal muscle

2021 ◽  
Vol 7 (4) ◽  
pp. eabd9502 ◽  
Author(s):  
Zhaowei Chen ◽  
Binjie Li ◽  
Ren-Zhi Zhan ◽  
Lingjun Rao ◽  
Nenad Bursac
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Inflammatory Diseases ◽  
Interferon Γ ◽  
Janus Kinase ◽  
Muscle Disease ◽  
Ifn Γ ◽  
Anti Inflammatory ◽  
Underlying Mechanisms

Chronic inflammatory diseases often lead to muscle wasting and contractile deficit. While exercise can have anti-inflammatory effects, the underlying mechanisms remain unclear. Here, we used an in vitro tissue-engineered model of human skeletal muscle (“myobundle”) to study effects of exercise-mimetic electrical stimulation (E-stim) on interferon-γ (IFN-γ)–induced muscle weakness. Chronic IFN-γ treatment of myobundles derived from multiple donors induced myofiber atrophy and contractile loss. E-stim altered the myobundle secretome, induced myofiber hypertrophy, and attenuated the IFN-γ–induced myobundle wasting and weakness, in part by down-regulating JAK (Janus kinase)/STAT1 (signal transducer and activator of transcription 1) signaling pathway amplified by IFN-γ. JAK/STAT inhibitors fully prevented IFN-γ–induced myopathy, confirming the critical roles of STAT1 activation in proinflammatory action of IFN-γ. Our results reveal a previously unknown mechanism of the cell-autonomous anti-inflammatory effects of muscle exercise and establish the utility of human myobundle platform for studies of inflammatory muscle disease and therapy.

scholarly journals Contribution of Nonesterified Fatty Acids to Mitogen-Activated Protein Kinase Activation in Human Skeletal Muscle During Endurance Exercise

2013 ◽  
Vol 23 (3) ◽  
pp. 201-209 ◽  
Author(s):  
Hermann Zbinden-Foncea ◽  
Luc J. C. van Loon ◽  
Jean-Marc Raymackers ◽  
Marc Francaux ◽  
Louise Deldicque
Keyword(s):  
Skeletal Muscle ◽  
Fatty Acids ◽  
Endurance Exercise ◽  
Healthy Subjects ◽  
Human Skeletal Muscle ◽  
Mitogen Activated Protein Kinase ◽  
Plasma Nefa ◽  
Nonesterified Fatty Acids ◽  
Patients With Diabetes ◽  
Mitogen Activated Protein

Mitogen-activated protein kinase (MAPK) pathways are activated in skeletal muscle during endurance exercise, but the upstream molecular events are incompletely resolved. As an increase in plasma nonesterified fatty acids (NEFA) is a common feature of long-lasting exercise, the authors tested the hypothesis that NEFA contribute to the activation of MAPK during endurance exercise. Acipimox was used before and during endurance exercise to prevent the elevation of plasma NEFA levels in healthy subjects and patients with diabetes. In 2 separate studies, healthy subjects cycled for 2 hr and patients with diabetes for 1 hr at 50% Wmax. In control conditions, plasma NEFA concentrations increased from 0.35 to 0.90 mM during exercise in healthy subjects and from 0.55 to 0.70 mM in patients with diabetes (p < .05). Phosphorylation states of extracellularly regulated kinase 1 and 2 (ERK1/2), p38, and c-Jun NH2-terminal kinases (JNK) were significantly increased after exercise in the vastus lateralis in both groups. Acipimox blocked the increase in plasma NEFA concentrations and almost completely repressed any rise in ERK1/2 and p38 but not in JNK. In conclusion, the data support a role for plasma NEFA in the activation of p38 and ERK1/2 in skeletal-muscle tissue of healthy and diabetic subjects during endurance exercise. Further investigation will be required to determine the molecular link between NEFA and MAPK activation during exercise in human skeletal muscle.

TGFß regulates metabolism of human skeletal muscle cells by miRNAs

2018 ◽  
Author(s):  
S Höckele ◽  
P Huypens ◽  
C Hoffmann ◽  
T Jeske ◽  
M Hastreiter ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells

IRAK-1/IRS-1 Signaling Cross Talk in Human Skeletal Muscle—Role in Insulin Resistance

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 159-OR
Author(s):  
THEODORE P. CIARALDI ◽  
SUNDER MUDALIAR ◽  
LIWU LI ◽  
ROSARIO SCALIA ◽  
XIAO JIAN SUN ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Cross Talk ◽  
Human Skeletal Muscle
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