Enhanced mucosal and systemic immune responses following intravaginal immunization with human papillomavirus 16 L1 virus-like particle vaccine in thermosensitive mucoadhesive delivery systems

2003 ◽  
Vol 70 (4) ◽  
pp. 633-641 ◽  
Author(s):  
Jeong-Sook Park ◽  
Yu-Kyoung Oh ◽  
Min-Jeong Kang ◽  
Chong-Kook Kim
Keyword(s):  
Human Papillomavirus ◽  
Immune Responses ◽  
Delivery Systems ◽  
Human Papillomavirus 16 ◽  
Virus Like Particle

scholarly journals Head-to-Head Comparison of Modular Vaccines Developed Using Different Capsid Virus-Like Particle Backbones and Antigen Conjugation Systems

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 539
Author(s):  
Laurits Fredsgaard ◽  
Louise Goksøyr ◽  
Susan Thrane ◽  
Kara-Lee Aves ◽  
Thor G. Theander ◽  
...  
Keyword(s):  
Immune Response ◽  
Human Papillomavirus ◽  
Immune Responses ◽  
Capsid Protein ◽  
Major Capsid Protein ◽  
Molecular Scaffolds ◽  
Conjugation System ◽  
Virus Like Particles ◽  
Virus Like Particle ◽  
Specific Igg

Capsid virus-like particles (cVLPs) are used as molecular scaffolds to increase the immunogenicity of displayed antigens. Modular platforms have been developed whereby antigens are attached to the surface of pre-assembled cVLPs. However, it remains unknown to what extent the employed cVLP backbone and conjugation system may influence the immune response elicited against the displayed antigen. Here, we performed a head-to-head comparison of antigen-specific IgG responses elicited by modular cVLP-vaccines differing by their employed cVLP backbone or conjugation system, respectively. Covalent antigen conjugation (i.e., employing the SpyTag/SpyCatcher system) resulted in significantly higher antigen-specific IgG titers compared to when using affinity-based conjugation (i.e., using biotin/streptavidin). The cVLP backbone also influenced the antigen-specific IgG response. Specifically, vaccines based on the bacteriophage AP205 cVLP elicited significantly higher antigen-specific IgG compared to corresponding vaccines using the human papillomavirus major capsid protein (HPV L1) cVLP. In addition, the AP205 cVLP platform mediated induction of antigen-specific IgG with a different subclass profile (i.e., higher IgG2a and IgG2b) compared to HPV L1 cVLP. These results demonstrate that the cVLP backbone and conjugation system can individually affect the IgG response elicited against a displayed antigen. These data will aid the understanding and process of tailoring modular cVLP vaccines to achieve improved immune responses.

Enhanced humoral and cellular immune responses after sublingual immunization against human papillomavirus 16 L1 protein with adjuvants

Vaccine ◽  
2010 ◽  
Vol 28 (14) ◽  
pp. 2598-2606 ◽  
Author(s):  
Hee-Jeong Cho ◽  
Ji-Yeon Kim ◽  
Young Lee ◽  
Jung Mogg Kim ◽  
Young Bong Kim ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Immune Responses ◽  
Human Papillomavirus 16 ◽  
Cellular Immune Responses ◽  
Cellular Immune ◽  
L1 Protein

A Novel Human Papillomavirus 16 L1 Pentamer-Loaded Hybrid Particles Vaccine System: Influence of Size on Immune Responses

2018 ◽  
Vol 10 (42) ◽  
pp. 35745-35759 ◽  
Author(s):  
Chengcheng Jia ◽  
Tingyuan Yang ◽  
Yongjiang Liu ◽  
Ali Zhu ◽  
Fei Yin ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Immune Responses ◽  
Hybrid Particles ◽  
Human Papillomavirus 16

scholarly journals Safety and Immunogenicity Trial in Adult Volunteers of a Human Papillomavirus 16 L1 Virus-Like Particle Vaccine

2001 ◽  
Vol 93 (4) ◽  
pp. 284-292 ◽  
Author(s):  
C. D. Harro ◽  
Y.-Y. S. Pang ◽  
R. B. S. Roden ◽  
A. Hildesheim ◽  
Z. Wang ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Human Papillomavirus 16 ◽  
Virus Like Particle ◽  
Adult Volunteers

Immune responses induced by lower airway mucosal immunisation with a human papillomavirus type 16 virus-like particle vaccine

Vaccine ◽  
2005 ◽  
Vol 23 (28) ◽  
pp. 3634-3641 ◽  
Author(s):  
D NARDELLIHAEFLIGER ◽  
F LURATI ◽  
D WIRTHNER ◽  
F SPERTINI ◽  
J SCHILLER ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Immune Responses ◽  
Lower Airway ◽  
Human Papillomavirus Type 16 ◽  
Virus Like Particle ◽  
Mucosal Immunisation

scholarly journals Priming of Human Papillomavirus Type 11-Specific Humoral and Cellular Immune Responses in College-Aged Women with a Virus-Like Particle Vaccine

2002 ◽  
Vol 76 (15) ◽  
pp. 7832-7842 ◽  
Author(s):  
Rebecca T. Emeny ◽  
Cosette M. Wheeler ◽  
Kathrin U. Jansen ◽  
William C. Hunt ◽  
Tong-Ming Fu ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Immune Responses ◽  
Antibody Titer ◽  
Mononuclear Cells ◽  
Geometric Mean ◽  
Interleukin 2 ◽  
Cellular Immune Responses ◽  
Virus Like Particle ◽  
Ifn Γ ◽  
Cellular Immune

ABSTRACT In this study, we evaluated the potency of a human papillomavirus (HPV) virus-like particle (VLP)-based vaccine at generating HPV type 11 (HPV-11)-specific cellular and humoral immune responses in seronegative women. The vaccine was administered by intramuscular immunizations at months 0, 2, and 6. A fourth immunization was administered to approximately half of the women at month 12. All vaccine recipients had positive HPV-11 VLP-specific lymphoproliferative responses at month 3 following the second immunization (geometric mean lymphoproliferative stimulation index [SI] = 28.4; 95% confidence interval [CI] = 16.9 to 48.0) and HPV-11 VLP-specific antibody titers following the first immunization at month 1 (geometric mean antibody titer = 53.9 milli-Merck units/ml, 95% CI, 34.8 to 83.7). In contrast, lymphoproliferative and antibody titer responses were never detected in the participants who received placebo. Relatively homogeneous lymphoproliferative responses were observed in all vaccinated women. The mean lymphoproliferative SI of the vaccinated group over the first 12 months of the study was 7.6-fold greater than that of the placebo group following the initial immunization. The cellular immune responses generated by VLP immunization were both Th1 and Th2, since peripheral blood mononuclear cells from vaccinees, but not placebo recipients, secreted interleukin 2 (IL-2), IL-5, and gamma interferon (IFN-γ) in response to in vitro stimulation with HPV-11 VLP. The proliferation-based SI was moderately correlated with IFN-γ production and significantly correlated with IL-2 production after the third immunization (P = 0.078 and 0.002, respectively). The robust lymphoproliferative responses were specific for HPV-11, since SIs generated against bovine papillomavirus and HPV-16 VLPs were not generally observed and when detected were similar pre- and postimmunization.

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