Effect of chronic morphine exposure on the synaptic plasma-membrane subproteome of rats: a quantitative protein profiling study based on isotope-coded affinity tags and liquid chromatography/mass spectrometry

2005 ◽  
Vol 40 (2) ◽  
pp. 169-175 ◽  
Author(s):  
Laszlo Prokai ◽  
Alevtina D. Zharikova ◽  
Stanley M. Stevens
Keyword(s):  
Mass Spectrometry ◽  
Plasma Membrane ◽  
Liquid Chromatography ◽  
Protein Profiling ◽  
Synaptic Plasma Membrane ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chronic Morphine ◽  
Affinity Tags ◽  
Chromatography Mass Spectrometry

scholarly journals Multiphasic strain differentiation of atypical mycobacteria from elephant trunk wash

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1367 ◽  
Author(s):  
Kok-Gan Chan ◽  
Mun Fai Loke ◽  
Bee Lee Ong ◽  
Yan Ling Wong ◽  
Kar Wai Hong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Repetitive Sequence ◽  
Protein Profiling ◽  
Phenotype Microarray ◽  
Whole Genome ◽  
Liquid Chromatography Mass Spectrometry ◽  
Strain Differentiation ◽  
Chromatography Mass Spectrometry ◽  
A Genome

Background.Two non-tuberculous mycobacterial strains, UM_3 and UM_11, were isolated from the trunk wash of captive elephants in Malaysia. As they appeared to be identical phenotypes, they were investigated further by conventional and whole genome sequence-based methods of strain differentiation.Methods.Multiphasic investigations on the isolates included species identification with hsp65 PCR-sequencing, conventional biochemical tests, rapid biochemical profiling using API strips and the Biolog Phenotype Microarray analysis, protein profiling with liquid chromatography-mass spectrometry, repetitive sequence-based PCR typing and whole genome sequencing followed by phylogenomic analyses.Results.The isolates were shown to be possibly novel slow-growing schotochromogens with highly similar biological and genotypic characteristics. Both strains have a genome size of 5.2 Mbp, G+C content of 68.8%, one rRNA operon and 52 tRNAs each. They qualified for classification into the same species with their average nucleotide identity of 99.98% and tetranucleotide correlation coefficient of 0.99999. At the subspecies level, both strains showed 98.8% band similarity in the Diversilab automated repetitive sequence-based PCR typing system, 96.2% similarity in protein profiles obtained by liquid chromatography mass spectrometry, and a genomic distance that is close to zero in the phylogenomic tree constructed with conserved orthologs. Detailed epidemiological tracking revealed that the elephants shared a common habitat eight years apart, thus, strengthening the possibility of a clonal relationship between the two strains.

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