Mapping glucose metabolites in the normal bovine lens: Evaluation and optimisation of a matrix‐assisted laser desorption/ionisation imaging mass spectrometry method

2020 ◽  
Vol 56 (4) ◽  
Author(s):  
Ali Zahraei ◽  
George Guo ◽  
Rebecca D. Perwick ◽  
Paul J. Donaldson ◽  
Nicholas J. Demarais ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Imaging Mass Spectrometry ◽  
Laser Desorption ◽  
Spectrometry Method ◽  
Mass Spectrometry Method ◽  
Bovine Lens ◽  
Glucose Metabolites ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Verification of a Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Method for Diagnostic Identification of High-Consequence Bacterial Pathogens: TABLE 1

2015 ◽  
Vol 54 (3) ◽  
pp. 764-767 ◽  
Author(s):  
Dobryan M. Tracz ◽  
Kym S. Antonation ◽  
Cindi R. Corbett
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Spectrometry Method ◽  
Ionization Time ◽  
Mass Spectrometry Method ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

We examined the utility of a single matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry method for the identification of security-sensitive biological agents (risk group 3 bacterial pathogens). The goal was 2-fold: to verify a method for inclusion into our scope of accreditation, and to assess the biological safety of extractions. We developed our sample flow to include a tube-based chemical extraction, followed by filtration, before processing on MALDI-TOF MS instruments in a containment level 2 laboratory.

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