scholarly journals Multiple-stage linear ion-trap with high resolution mass spectrometry towards complete structural characterization of phosphatidylethanolamines containing cyclopropane fatty acyl chain inLeishmania infantum

2014 ◽  
Vol 49 (3) ◽  
pp. 201-209 ◽  
Author(s):  
Fong-Fu Hsu ◽  
F. Matthew Kuhlmann ◽  
John Turk ◽  
Stephen M. Beverley
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Ion Trap ◽  
High Resolution Mass Spectrometry ◽  
Acyl Chain ◽  
Fatty Acyl ◽  
Fatty Acyl Chain ◽  
Linear Ion Trap ◽  
Resolution Mass

scholarly journals Multiple-stage Precursor Ion Separation and High Resolution Mass Spectrometry toward Structural Characterization of 2,3-Diacyltrehalose Family from Mycobacterium tuberculosis

Separations ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 4 ◽  
Author(s):  
Cheryl Frankfater ◽  
Robert Abramovitch ◽  
Georgiana Purdy ◽  
John Turk ◽  
Laurent Legentil ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mycobacterium Tuberculosis ◽  
High Resolution ◽  
Structural Characterization ◽  
High Resolution Mass Spectrometry ◽  
Complex Mixture ◽  
Fatty Acyl ◽  
High Resolution Mass ◽  
Resolution Mass

Mass spectrometry (MS)-based precursor ion isolation, collision-induced dissociation (CID) fragmentation, and detection using linear ion-trap multiple-stage mass spectrometry (LIT MSn) in combination with high resolution mass spectrometry (HRMS) provides a unique tool for structural characterization of complex mixture without chromatographic separation. This approach permits not only separation of various lipid families and their subfamilies, but also stereoisomers, thereby, revealing the structural details. In this report, we describe the LIT MSn approach to unveil the structures of a 2,3-diacyl trehalose (DAT) family isolated from the cell envelope of Mycobacterium tuberculosis, in which more than 30 molecular species, and each species consisting of up to six isomeric structures were found. LIT MSn performed on both [M + Na]+ and [M + HCO2]− ions of DAT yield complimentary structural information for near complete characterization of the molecules, including the location of the fatty acyl substituents on the trehalose backbone. This latter information is based on the findings of the differential losses of the two fatty acyl chains in the MS2 and MS3 spectra; while the product ion spectra from higher stage LIT MSn permit confirmation of the structural assignment.

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