Microscale enzyme reactors comprising gold nanoparticles with immobilized trypsin for efficient protein digestion

2013 ◽  
Vol 48 (12) ◽  
pp. 1281-1284 ◽  
Author(s):  
Muhammad Safdar ◽  
Jens Sproß ◽  
Janne Jänis
Keyword(s):  
Gold Nanoparticles ◽  
Protein Digestion ◽  
Enzyme Reactors ◽  
Immobilized Trypsin

Facile synthesis of enzyme–inorganic hybrid nanoflowers and their application as an immobilized trypsin reactor for highly efficient protein digestion

RSC Advances ◽  
2014 ◽  
Vol 4 (27) ◽  
pp. 13888-13891 ◽  
Author(s):  
Zian Lin ◽  
Yun Xiao ◽  
Ling Wang ◽  
Yuqing Yin ◽  
Jiangnan Zheng ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Immobilized Enzyme ◽  
Protein Digestion ◽  
Enzyme Reactor ◽  
Facile Synthesis ◽  
Inorganic Hybrid ◽  
Immobilized Enzyme Reactor ◽  
Highly Efficient ◽  
Novel Approach ◽  
Immobilized Trypsin

Hybrid nanoflowers were synthesized by a novel approach. The nanoflowers exhibited an enhanced enzymatic activity and can be used as an immobilized enzyme reactor (IMER) for highly efficient protein digestion.

Rational synthesis of MoS2-based immobilized trypsin for rapid and effective protein digestion

Talanta ◽  
2018 ◽  
Vol 179 ◽  
pp. 393-400 ◽  
Author(s):  
Chaoshuang Xia ◽  
Heping Wang ◽  
Fenglong Jiao ◽  
Fangyuan Gao ◽  
Qiong Wu ◽  
...  
Keyword(s):  
Protein Digestion ◽  
Immobilized Trypsin

LC–MS/MS method for denosumab quantitation in human serum with rapid protein digestion using immobilized trypsin

Bioanalysis ◽  
2018 ◽  
Vol 10 (18) ◽  
pp. 1501-1510 ◽  
Author(s):  
Hiroaki Shida ◽  
Takafumi Naito ◽  
Kaito Shibata ◽  
Yasuhide Yamada ◽  
Junichi Kawakami
Keyword(s):  
Human Serum ◽  
Protein Digestion ◽  
Immobilized Trypsin
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