scholarly journals Modelling of the gas-phase phosphate group loss and rearrangement in phosphorylated peptides

2011 ◽  
Vol 46 (9) ◽  
pp. 949-955 ◽  
Author(s):  
Marko Rožman
Keyword(s):  
Gas Phase ◽  
Phosphate Group ◽  
Phosphorylated Peptides

Adsorption Properties of Phospho-Polypeptide on Synthetic Hydroxyapatite Biomaterials

2011 ◽  
Vol 130-134 ◽  
pp. 1445-1447
Author(s):  
Xiao Qing Shen ◽  
Yan Jun Du ◽  
Yuan Yuan Zhou ◽  
Si Wang ◽  
Bin Liu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Adsorption Isotherms ◽  
Adsorption Properties ◽  
Phosphate Group ◽  
Adsorption Affinity ◽  
Phosphorylated Peptides ◽  
Biological Composites ◽  
Solid Foundation ◽  
Synthetic Hydroxyapatite

This article dealt with the effect of phosphorylated peptides and peptides onto hydroxyapatite (HAP) biomaterials by considering their influence when they were present at the synthetic HAP interface. The adsorption isotherms adequately fitted the Langmuir shape, and from the comparision between two different adsorbates, the results of the investigation indicated that the phosphate group greatly enhanced the adsorption affinity. The results obtained layed the solid foundation for further research on the regulating function of phosphorylated amino acids with hydroxyapatite biological composites.

scholarly journals Detecting the Site of Phosphorylation in Phosphopeptides without Loss of Phosphate Group Using MALDI TOF Mass Spectrometry

2008 ◽  
Vol 3 ◽  
pp. ACI.S497 ◽  
Author(s):  
Medicharla V. Jagannadham ◽  
Ramakrishnan Nagaraj
Keyword(s):  
Mass Spectrometry ◽  
Negative Ions ◽  
Maldi Mass Spectrometry ◽  
Phosphate Group ◽  
Maldi Tof Mass Spectrometry ◽  
Linear Mode ◽  
Maldi Tof ◽  
Phosphorylated Peptides ◽  
Delayed Extraction ◽  
Phosphate Groups

Phosphopeptides with one and four phosphate groups were characterized by MALDI mass spectrometry. The molecular ion of monophosphopeptide could be detected both as positive and negative ions by MALDI TOF with delayed extraction (DE) and in the reflector mode. The tetraphospho peptide could be detected in linear mode. When MS/MS spectra of the monophospho peptides were obtained in a MALDI TOF TOF instrument by CID, b and y ions with the intact phosphate group were observed, in addition the b and y ions without the phosphate group. Our study indicates that it is possible to detect phosphorylated peptides with out the loss of phosphate group by MALDI TOF as well as MALDI TOF TOF instruments with delayed extraction and in the reflector mode.

scholarly journals Gas-Phase Rearrangement in Lysine Phosphorylated Peptides During Electron-Transfer Dissociation Tandem Mass Spectrometry

2015 ◽  
Vol 87 (14) ◽  
pp. 6990-6994 ◽  
Author(s):  
Jordi Bertran-Vicente ◽  
Michael Schümann ◽  
Christian P. R. Hackenberger ◽  
Eberhard Krause
Keyword(s):  
Mass Spectrometry ◽  
Electron Transfer ◽  
Tandem Mass Spectrometry ◽  
Gas Phase ◽  
Electron Transfer Dissociation ◽  
Tandem Mass ◽  
Phosphorylated Peptides

Residual Gas Reactions in the Electron Microscope: I. Qualitative Observations on the Water Gas Reaction

1968 ◽  
Vol 26 ◽  
pp. 292-293
Author(s):  
Richard E. Hartman ◽  
Roberta S. Hartman ◽  
Peter L. Ramos
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Gas Phase ◽  
Absolute Temperature ◽  
Residual Gas ◽  
Gas Reactions ◽  
Image Position ◽  
The Right ◽  
Water Gas ◽  
Thinning Rate

The action of water and the electron beam on organic specimens in the electron microscope results in the removal of oxidizable material (primarily hydrogen and carbon) by reactions similar to the water gas reaction .which has the form:The energy required to force the reaction to the right is supplied by the interaction of the electron beam with the specimen.The mass of water striking the specimen is given by:where u = gH2O/cm2 sec, PH2O = partial pressure of water in Torr, & T = absolute temperature of the gas phase. If it is assumed that mass is removed from the specimen by a reaction approximated by (1) and that the specimen is uniformly thinned by the reaction, then the thinning rate in A/ min iswhere x = thickness of the specimen in A, t = time in minutes, & E = efficiency (the fraction of the water striking the specimen which reacts with it).

Integration of Core-Edge Spectroscopy Methods for the Study of Polymers

1996 ◽  
Vol 54 ◽  
pp. 154-155
Author(s):  
E. G. Rightor
Keyword(s):  
Excited State ◽  
Polymer Blends ◽  
Gas Phase ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
Electronic States ◽  
Core Electron ◽  
Bulk Solids ◽  
Development Application

Core edge spectroscopy methods are versatile tools for investigating a wide variety of materials. They can be used to probe the electronic states of materials in bulk solids, on surfaces, or in the gas phase. This family of methods involves promoting an inner shell (core) electron to an excited state and recording either the primary excitation or secondary decay of the excited state. The techniques are complimentary and have different strengths and limitations for studying challenging aspects of materials. The need to identify components in polymers or polymer blends at high spatial resolution has driven development, application, and integration of results from several of these methods.

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