Elucidation of the mass fragmentation pathways of potato glycoalkaloids and aglycons using Orbitrap mass spectrometry

2010 ◽  
Vol 45 (9) ◽  
pp. 1019-1025 ◽  
Author(s):  
Michael G. Cahill ◽  
Giovanni Caprioli ◽  
Sauro Vittori ◽  
Kevin J. James
Keyword(s):  
Mass Spectrometry ◽  
Fragmentation Pathways ◽  
Mass Fragmentation ◽  
Orbitrap Mass Spectrometry ◽  
Potato Glycoalkaloids

scholarly journals Elucidation of the Mass Fragmentation Pathways of Tomatidine and β1-Hydroxytomatine using Orbitrap Mass Spectrometry

2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Giovanni Caprioli ◽  
Michael Cahill ◽  
Serena Logrippo ◽  
Kevin James
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectra ◽  
Ion Trap ◽  
Biological Effects ◽  
Biologically Active ◽  
Fragmentation Pathways ◽  
Multi Stage ◽  
Orbitrap Mass Spectrometry ◽  
Characteristic Fragmentation ◽  
Collisional Induced Dissociation

Tomatoes, members of the Solanaceae plant family, produce biologically active secondary metabolites, including glycoalkaloids, which may have both adverse and beneficial biological effects. Using the linear ion trap (LIT) mass spectrometry, multi-stage collision induced dissociation (CID) experiments (MSn) were performed to elucidate characteristic fragmentation pathways of the glycoalkaloid, tomatidine and of β1-hydroxytomatine. High resolution with high accuracy mass analysis using an Orbitrap fourier transform MS with higher-energy collisional induced dissociation (HCD) was used to produce mass spectra data across a wide spectral range for confirmation of proposed ion structures and formulae.

Investigation on fragmentation pathways of bisphenols by using electrospray ionization Orbitrap mass spectrometry

2016 ◽  
Vol 30 (16) ◽  
pp. 1901-1913 ◽  
Author(s):  
Hongzhi Zhao ◽  
Li Xiang ◽  
Jiufeng Li ◽  
Zhiyi Yang ◽  
Jing Fang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Fragmentation Pathways ◽  
Orbitrap Mass Spectrometry

scholarly journals Comparative Research of Chemical Profiling in Different Parts of Fissistigma oldhamii by Ultra-High-Performance Liquid Chromatography Coupled with Hybrid Quadrupole-Orbitrap Mass Spectrometry

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 960
Author(s):  
Haibo Hu ◽  
Yau Lee-Fong ◽  
Jinnian Peng ◽  
Bin Hu ◽  
Jialin Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Scientific Development ◽  
Multivariate Statistical ◽  
Accurate Identification ◽  
Orbitrap Mass Spectrometry ◽  
Q Exactive

The roots of Fissistigma oldhamii (FO) are widely used as medicine with the effect of dispelling wind and dampness, promoting blood circulation and relieving pains, and its fruits are considered delicious. However, Hakka people always utilize its above-ground parts as a famous folk medicine, Xiangteng, with significant differences from literatures. Studies of chemical composition showed there were multiple aristolactams that possessed high nephrotoxicity, pending evaluation research about their distribution in FO. In this study, a sensitive, selective, rapid and reliable method was established to comparatively perform qualitative and semi-quantitative analysis of the constituents in roots, stems, leaves, fruits and insect galls, using an Ultra-High-Performance Liquid Chromatography coupled with Hybrid Quadrupole Orbitrap Mass Spectrometry (UPLC-Q-Exactive Orbitrap MS, or Q-Exactive for short). To make more accurate identification and comparison of FO chemicals, all MS data were aligned and screened by XCMS, then their structures were elucidated according to MSn ion fragments between the detected and standards, published ones or these generated by MS fragmenter. A total of 79 compounds were identified, including 33 alkaloids, 29 flavonoids, 11 phenylpropanoids, etc. There were 54 common components in all five parts, while another 25 components were just detected in some parts. Six toxic aristolactams were detected in this experiment, including aristolactam AII, AIIIa, BII, BIII, FI and FII, of which the relative contents in above-ground stems were much higher than roots. Meanwhile, multivariate statistical analysis was performed and showed significant differences both in type and content of the ingredients within all FO parts. The results implied that above-ground FO parts should be carefully valued for oral administration and eating fruits. This study demonstrated that the high-resolution mass spectrometry coupled with multivariate statistical methods was a powerful tool in compound analysis of complicated herbal extracts, and the results provide the basis for its further application, scientific development of quality standard and utilization.

Analysis of lipidomics profile of rice and changes during storage by UPLC-Q-extractive orbitrap mass spectrometry

2021 ◽  
Vol 142 ◽  
pp. 110214
Author(s):  
Dong Zhang ◽  
Xiaoliang Duan ◽  
Bo Shang ◽  
Yu Hong ◽  
Hui Sun
Keyword(s):  
Mass Spectrometry ◽  
Orbitrap Mass Spectrometry
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