scholarly journals Performance of HPLC/MS microchips in isocratic and gradient elution modes

2010 ◽  
Vol 45 (3) ◽  
pp. 313-320 ◽  
Author(s):  
Steffen Ehlert ◽  
Lukas Trojer ◽  
Martin Vollmer ◽  
Tom van de Goor ◽  
Ulrich Tallarek
Keyword(s):  
Gradient Elution ◽  
Isocratic And Gradient Elution

scholarly journals Determination of 25 Low Molecular Weight Carbohydrates in Tobacco by High Performance Ion Chromatography

1994 ◽  
Vol 16 (2) ◽  
pp. 77-84 ◽  
Author(s):  
MC Ramusino ◽  
BS Dattilo ◽  
A Lucibello ◽  
SG Rossi
Keyword(s):  
Molecular Weight ◽  
High Performance ◽  
Chromatographic Method ◽  
Gradient Elution ◽  
Low Molecular Weight ◽  
Qualitative And Quantitative ◽  
Naoh Solution ◽  
Modified Polystyrene ◽  
Isocratic And Gradient Elution

AbstractA High Performance Ion Chromatographic method for the qualitative and quantitative determination of low molecular weight carbohydrates in tobacco leaf is described. The carbohydrates were separated by running isocratic or gradient elutions on columns packed with amino-modified polystyrene divinylbenzene phases, using 0.1 N NaOH as eluent. The compounds were detected by a Pulsed Amperometric Detector, after post-column addition of 1 N NaOH solution. Twenty-two and twenty-four carbohydrates out of twenty-five were separated by isocratic and gradient elution, respectively. Some mono and disaccharides were identified in tobacco samples by their retention times and some quantitative determinations of glucose, fructose, inositol and saccharose were carried out using different varieties of tobacco.

scholarly journals Strategic Combination of Isocratic and Gradient Elution for Simultaneous Separation of Polar Compounds in Traditional Chinese Medicines by HPLC

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Mei-Tuo Zhang ◽  
Xiao-Xue Ye ◽  
Wei Lan ◽  
Yan-Ling Yang ◽  
Tsunghsueh Wu ◽  
...  
Keyword(s):  
High Performance ◽  
Gradient Elution ◽  
Polar Compounds ◽  
Hplc Method ◽  
Traditional Chinese Medicines ◽  
Chinese Medicines ◽  
Polar Components ◽  
Simultaneous Separation ◽  
Gradient Separation ◽  
Isocratic And Gradient Elution

A simple high-performance liquid chromatography (HPLC) method for the simultaneous separation of the highly polar and weakly polar components of traditional Chinese medicines was developed via a strategic combination of isocratic and gradient elution methods. Liu-Shen-Wan and Liu-Wei-Di-Huang-Wan were used as representative examples of traditional Chinese medicines. This is the first time that 6 components of varying degrees of polarity in Liu-Shen-Wan had been successfully resolved in a single chromatographic run using an ultraviolet-visible detector with a fixed wavelength of 296 nm. In contrast to conventional gradient separation methods, this novel method offered a viable route for separation of the highly and weakly polar fractions simultaneously, thus greatly reducing the time and cost of analysis. This method therefore provides a more efficient way to determine the polar components present in traditional Chinese medicines. It would find potential application in drug screening, drug authentication, and product quality control.

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