Polymeric integrated selective enrichment target (ISET) for solid-phase-based sample preparation in MALDI–TOF MS

2007 ◽  
Vol 42 (11) ◽  
pp. 1445-1452 ◽  
Author(s):  
Simon Ekström ◽  
Lars Wallman ◽  
Göran Helldin ◽  
Johan Nilsson ◽  
György Marko-Varga ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Solid Phase ◽  
Maldi Tof Ms ◽  
Selective Enrichment ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Hexagonal Mesoporous Silica as a Rapid, Efficient and Versatile Tool for MALDI-TOF MS Sample Preparation in Clinical Peptidomics Analysis: A Pilot Study

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2311
Author(s):  
Rosa Terracciano ◽  
Mariaimmacolata Preianò ◽  
Giuseppina Maggisano ◽  
Corrado Pelaia ◽  
Rocco Savino
Keyword(s):  
Synovial Fluid ◽  
Mesoporous Silica ◽  
Sample Preparation ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Versatile Tool ◽  
Tof Ms

Improvement in high-throughput MALDI-TOF MS analysis requires practical and efficient sample preparation protocols for high acquisition rates. The use of hexagonal mesoporous silica (HMS) sorbents in combination with MALDI-TOF MS was explored as a versatile tool for peptidomic profiling of clinical specimens difficult to process, but considered important sources of disease biomarkers: synovial fluid and sputum. A rapid and efficient procedure, based on dispersive solid-phase extraction of peptides using commercially available wormhole mesostructured HMS, was tested for: a) pre-concentration of standard peptides in serially diluted solution up to the sub-nanomolar range; b) peptidome profiling of sputum and synovial fluid. The use of HMS, as dispersed sponges, significantly amplified the peptidic repertoire of sputum and synovial fluid by excluding from the adsorptive process large size proteins, which mask and/or suppress peptidome signals. The protocol proposed, as dispersive solid phase extraction, ensures good analytical performances. Moreover, it is economical and rapid, as it avoids the use of less reproducible and prolonged sample preparation procedures, such as the use of ultrafiltration filter devices. These findings may contribute to defining a high-throughput screening MS-based platform for monitoring key peptidic features of difficult to analyse bodily fluids in a clinical setting.

Development of magnetic LuPO4 microspheres for highly selective enrichment and identification of phosphopeptides for MALDI-TOF MS analysis

2015 ◽  
Vol 3 (48) ◽  
pp. 9330-9339 ◽  
Author(s):  
Xing-yu Long ◽  
Qun Song ◽  
Hong-zhen Lian
Keyword(s):  
Core Shell ◽  
Maldi Tof Ms ◽  
Selective Enrichment ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Lichee-like core–shell structured magnetic lutetium phosphate (Fe3O4@LuPO4) affinity microspheres were synthesized, characterized and successfully applied to enrich phosphopeptides.

MALDI-TOF MS and its requirements for fungal identification.

2021 ◽  
pp. 119-140
Author(s):  
Cledir Santos ◽  
Paula Galeano ◽  
Reginaldo Lima Neto ◽  
Manoel Marques Evangelista Oliveira ◽  
Nelson Lima
Keyword(s):  
Sample Preparation ◽  
Black Box ◽  
Data Sets ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Fungal Identification ◽  
Identification Of Fungi ◽  
Tof Ms

Abstract Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is now used as a routine technique for the fast and reliable identification of fungi at the species level and, currently, it represents an important phenotypic methodology based on proteomic profiles. The main limitations to MALDI-TOF MS for fungal identification are related to sample quality (e.g. quality of biological material such as rigidity or pigmentation of cell walls), sample preparation (e.g. the myriad of sample preparation methodologies that deliver different data sets to different MALDI-TOF MS databases) and the databases themselves (e.g. the 'black-box' commercial databases). This chapter presents an overview and discussion of the use of MALDI-TOF MS for fungal identification. The major known limitations of the technique for fungal taxonomy, and how to overcome these, are also discussed.

Solid phase syntheses of peptoid like arylureido compounds and sequencing of isobars without molecular encoding

2019 ◽  
Vol 17 (17) ◽  
pp. 4204-4207
Author(s):  
Kanwal Asif ◽  
Gregory L. O'Brien ◽  
Scott M. Goodman ◽  
Sujit Suwal
Keyword(s):  
Solid Phase ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Isobaric arylureido peptoids undergo SS1 and SS2 fragmentations in MALDI-TOF MS/MS, thus offering a sequencing mechanism for arylureides without molecular encoding.

scholarly journals Testing the Applicability of MALDI-TOF MS as an Alternative Stock Identification Method in a Cryptic Species Complex

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3214
Author(s):  
Gabor Maasz ◽  
Zita Zrínyi ◽  
Istvan Fodor ◽  
Nóra Boross ◽  
Zoltán Vitál ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Cryptic Species ◽  
Species Complex ◽  
Intraspecific Variability ◽  
Clove Oil ◽  
Term Survival ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Knowledge of intraspecific variability of a certain species is essential for their long-term survival and for the development of conservation plans. Nowadays, molecular/genetic methods are the most frequently used for this purpose. Although, the Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) technique has become a promising alternative tool to specify intraspecific variability, there is a lack of information about the limitations of this method, and some methodological issues need to be resolved. Towards this goal, we tested the sensitivity of this method on an intraspecific level, using genetically identified individuals of a cryptic fish species complex collected from five distinct populations. Additionally, some methodologic issues, such as the effect of (1) delayed sample preparation, (2) clove oil anaesthetization, and (3) different tissue types (muscle, and brain) were investigated using the MS analysis results. Our results show that the delayed sample preparation has a fundamental effect on the result of MS analysis, while at the same time the clove oil did not affect the results considerably. Both the brain and muscle samples were usable for cryptic species identification, but in our opinion this method has limited applicability for population-level segregation. The application of MALDI-TOF MS to the exploitable toolkit of phylogenetic and taxonomic researches could be used to broaden conclusions.

scholarly journals MALDI-TOF MS-Based Analysis of Seed Proteins from Catalogue Varieties of Solanum lycopersicum/Lycopersicon esculentum

Horticulturae ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 48
Author(s):  
Michael Reeve
Keyword(s):  
Lycopersicon Esculentum ◽  
Sample Preparation ◽  
Solanum Lycopersicum ◽  
Seed Proteins ◽  
Impatiens Glandulifera ◽  
Invasive Weed ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Commercial Plant ◽  
Tof Ms

Matrix-assisted laser-desorption and ionization time-of-flight mass spectroscopy (MALDI-TOF MS) is a flexible technique for the analysis of protein-containing biological samples. Simple and inexpensive methods have previously been developed for MALDI-TOF MS sample preparation that are able to discriminate between Impatiens species that are closely related and also between regional biotypes of the invasive weed Impatiens glandulifera (Himalayan balsam) with leaf material and also seed material. The current article investigates whether MALDI-TOF MS, through acid-soluble protein ‘fingerprinting’, can be used to analyze plant seeds that result from intensive commercial plant-breeding activity. As an initial proof-of-concept study, tomato seeds from eleven seed-catalogue varieties (F1 Pink Baby Plum, F1 Fantasio, F1 Lizzano, F1 Sungold, F1 Tumbler, Faworyt, Golden Sunrise, Hundreds and Thousands, Indigo Rose, Moneymaker, and Red Alert), listed as Solanum lycopersicum or under the synonym Lycopersicon esculentum were analyzed using MALDI-TOF MS. Whilst peak-rich and highly-reproducible spectra were obtained, with very high Bruker comparison scores and low MALDI-TOF MS variance, sample-preparation variance, and seed-to-seed variance, the spectral differences between varieties were only slightly greater than the above combined variances, indicating very close similarity between all eleven varieties studied. These results are discussed in comparison with those previously observed with the naturally-evolving invasive species I. glandulifera.

Direct analysis of positive blood cultures using MALDI-TOF MS profiling and a fundamentally improved sample preparation technique

Pathology ◽  
2011 ◽  
Vol 43 ◽  
pp. S73-S74
Author(s):  
Guido Mix ◽  
Leith Fremlin ◽  
Thomas Maier ◽  
Beatrix Wegemann ◽  
Markus Kostrzewa
Keyword(s):  
Sample Preparation ◽  
Direct Analysis ◽  
Blood Cultures ◽  
Preparation Technique ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Sample Preparation Technique ◽  
Tof Ms
Sign in / Sign up

Export Citation Format

Share Document