Mass spectrometric characterization of low-molecular-mass color pI markers and their use for direct determination of pI value of proteins

2006 ◽  
Vol 41 (12) ◽  
pp. 1570-1577 ◽  
Author(s):  
Karel Mazanec ◽  
Karel Šlais ◽  
Josef Chmelík
Keyword(s):  
Molecular Mass ◽  
Direct Determination ◽  
Mass Spectrometric

Instrumentation for wet steam

2004 ◽  
Vol 218 (8) ◽  
pp. 811-842 ◽  
Author(s):  
A Kleitz ◽  
J M Dorey
Keyword(s):  
Liquid Phase ◽  
Liquid Film ◽  
Direct Determination ◽  
Dispersed Phase ◽  
Wet Steam ◽  
Turbine Performance ◽  
Chemical Measurements ◽  
Formation And Evolution

This paper is devoted to the measuring methods and instrumentation used in the wet-steam area for both aerodynamics measurements (pressure, temperature and velocity) taking into account the interaction between the vapour and the liquid phase, and the characterization of the liquid phase itself (dispersed phase and liquid film). The development of suitable instrumentation is an important part in understanding the formation and evolution of the liquid phase but is now more and more oriented towards practical industrial concerns, such as the direct determination of turbine performance by measuring exhaust wetness or chemical measurements relating to erosion in the transition zone inside the turbine. Emphasis has been given to techniques suitable for use in actual turbines.

scholarly journals Electrospray Ionization Mass Spectrometric Determination of the Molecular Mass of the ∼200-kDa Globin Dodecamer Subassemblies in Hexagonal Bilayer Hemoglobins

1999 ◽  
Vol 274 (40) ◽  
pp. 28206-28212 ◽  
Author(s):  
Brian N. Green ◽  
Robert S. Bordoli ◽  
Leonid G. Hanin ◽  
François H. Lallier ◽  
André Toulmond ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Electrospray Ionization ◽  
Mass Spectrometric ◽  
Ionization Mass ◽  
Mass Spectrometric Determination

scholarly journals Two-Scale Investigation of the Retention Behavior of a Well-Graded Mixed Soil

Geosciences ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 431
Author(s):  
Osvaldo Bottiglieri ◽  
Francesco Cafaro ◽  
Federica Cotecchia
Keyword(s):  
Measurement Techniques ◽  
Research Work ◽  
Direct Determination ◽  
Clayey Sand ◽  
Infiltration Process ◽  
Compacted Soils ◽  
Combined Strategy ◽  
Adsorption Phenomena

The hydraulic characterization of mixed compacted soils is helpful for the design of earthworks subjected to drying–wetting cycles. When the mixed soil is well-graded and made of both coarse and fine fractions, its matric suction may also be due to the short-range adsorption phenomena, as for the soil investigated in this research work. A silty–clayey sand was created by a mixing procedure and experimentally investigated at two different scales. Physical modeling of an infiltration process was performed, allowing an inverse numerical analysis to infer the water retention and the hydraulic conductivity functions of the soil, whereas element testing on soil specimens allowed direct determination of the same equations. In the article, problems related to the employed suction measurement techniques have been pointed out and discussed. By this two-scale combined strategy, features of the soil hydraulic behavior, such as the wetting collapse, the shrinkage during drying, and the loop of hysteresis, were also determined.

scholarly journals Leptin Binding and Binding Capacity in Serum

2000 ◽  
Vol 46 (3) ◽  
pp. 379-384 ◽  
Author(s):  
Michael Landt
Keyword(s):  
Adipose Tissue ◽  
Molecular Mass ◽  
Binding Capacity ◽  
Direct Determination ◽  
New Method ◽  
Degree Of Saturation ◽  
Free Form ◽  
Hplc Separation ◽  
Serum Leptin

Abstract Background: Leptin, a hormone produced primarily by adipose tissue, is known to be present in serum as both monomeric (free) and higher molecular mass (bound) forms, but little is known about the nature of the bound forms or physiological variation in binding capacity. Methods: A new method to quantify the free and bound forms was developed, based on HPLC separation and RIA quantification in chromatography fractions. Reanalysis of specimens after addition of exogenous leptin allowed direct determination of leptin-binding capacity and the degree of saturation of leptin-binding capacity. Results: HPLC chromatography fractionated serum leptin into both the free form and as a broad peak of 59–130 kDa. Several experiments were conducted to validate the new method. The concentrations of bound leptin in serum were 0.45–3.94 μg/L, and they increased as total leptin (reflecting adiposity) increased in 24 lean and obese volunteers. Leptin was readily dissociated from the bound fraction by competition from exogenous leptin. Rechromatography of the bound fraction led to dissociation of leptin, which was promoted by warming the sera before chromatography. Leptin-binding capacity was 1.8–5.3 μg/L; binding capacity was nearly constant over a range of total leptin concentrations of 2–10 μg/L, and slowly increased at higher total leptin concentrations. Saturation of binding capacity was low (15%) at very low total leptin concentrations (<5 μg/L), but rose quickly to a plateau near 80% at higher total leptin concentrations. Conclusions: The new method facilitates measurement of free and bound fractions of serum leptin, and is the first method measuring leptin-binding capacity. These experiments demonstrate that the concentration of bound leptin and leptin-binding capacity vary physiologically, with binding/binding capacity increasing with adiposity. Except in very lean individuals, binding capacity is nearly completely saturated.

Isotope Dilution–Total Evaporation–Thermal Ionization Mass Spectrometric Direct Determination of Radioactive Strontium-90 in Microdrop Samples

2020 ◽  
Vol 92 (24) ◽  
pp. 16058-16065
Author(s):  
Chihiro Ito ◽  
Ryoya Shimode ◽  
Takashi Miyazaki ◽  
Shigeyuki Wakaki ◽  
Katsuhiko Suzuki ◽  
...  
Keyword(s):  
Isotope Dilution ◽  
Direct Determination ◽  
Mass Spectrometric ◽  
Thermal Ionization ◽  
Ionization Mass ◽  
Total Evaporation ◽  
Strontium 90 ◽  
Thermal Ionization Mass
Sign in / Sign up

Export Citation Format

Share Document