Positive-contrast cellular MRI of embryonic stem cells for tissue regeneration using a highly efficientT1MRI contrast agent

2016 ◽  
Vol 44 (6) ◽  
pp. 1456-1463 ◽  
Author(s):  
Sadi Loai ◽  
Inga Haedicke ◽  
Zahra Mirzaei ◽  
Craig A. Simmons ◽  
Xiao-an Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Contrast Agent ◽  
Tissue Regeneration ◽  
Embryonic Stem ◽  
Positive Contrast ◽  
Cellular Mri

In vitro expansion of embryonic stem cells for progenitor isolation as a source of cells for tissue regeneration

2007 ◽  
Vol 205 (3) ◽  
pp. S65
Author(s):  
Paul J. Kokorowski ◽  
Laura Perin ◽  
Federico Tozzi ◽  
Casey Brewer ◽  
Sargis Sedrakyan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Tissue Regeneration ◽  
Embryonic Stem ◽  
In Vitro Expansion

scholarly journals An Efficient T1 Contrast Agent for Labeling and Tracking Human Embryonic Stem Cells on MRI

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Inga E. Haedicke ◽  
Sadi Loai ◽  
Hai-Ling Margaret Cheng
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Contrast Agent ◽  
Human Embryonic Stem Cells ◽  
Cell Tracking ◽  
Low Dose ◽  
Unmet Need ◽  
Embryonic Stem

Noninvasive cell tracking in vivo has the potential to advance stem cell-based therapies into the clinic. Magnetic resonance imaging (MRI) provides an excellent image-guidance platform; however, existing MR cell labeling agents are fraught with limited specificity. To address this unmet need, we developed a highly efficient manganese porphyrin contrast agent, MnEtP, using a two-step synthesis. In vitro MRI at 3 Tesla on human embryonic stem cells (hESCs) demonstrated high labeling efficiency at a very low dose of 10 µM MnEtP, resulting in a four-fold lower T1 relaxation time. This extraordinarily low dose is ideal for labeling large cell numbers required for large animals and humans. Cell viability and differentiation capacity were unaffected. Cellular manganese quantification corroborated MRI findings, and the agent localized primarily on the cell membrane. In vivo MRI of transplanted hESCs in a rat demonstrated excellent sensitivity and specificity of MnEtP for noninvasive stem cell tracking.

scholarly journals A manganese porphyrin-based T1 contrast agent for cellular MR imaging of human embryonic stem cells

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Andrei Venter ◽  
Daniel A. Szulc ◽  
Sadi Loai ◽  
Tameshwar Ganesh ◽  
Inga E. Haedicke ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mr Imaging ◽  
Contrast Agent ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Manganese Porphyrin ◽  
T1 Contrast Agent ◽  
T1 Contrast
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