Design and use of multi-affinity surfaces in biomolecular interaction analysis-mass spectrometry (BIA/MS): a step toward the design of SPR/MS arrays

2003 ◽  
Vol 16 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Dobrin Nedelkov ◽  
Randall W. Nelson
Keyword(s):  
Mass Spectrometry ◽  
Interaction Analysis ◽  
Biomolecular Interaction ◽  
Biomolecular Interaction Analysis

scholarly journals Peer Reviewed: Biomolecular Interaction Analysis Mass Spectrometry.

2000 ◽  
Vol 72 (11) ◽  
pp. 404 A-411 A ◽  
Author(s):  
Randall W. Nelson ◽  
Dobrin Nedelkov ◽  
Kemmons A. Tubbs
Keyword(s):  
Mass Spectrometry ◽  
Interaction Analysis ◽  
Biomolecular Interaction ◽  
Biomolecular Interaction Analysis

BIA/MS: Interfacing Biomolecular Interaction Analysis with Mass Spectrometry

1997 ◽  
Vol 244 (1) ◽  
pp. 124-132 ◽  
Author(s):  
Jennifer R. Krone ◽  
Randall W. Nelson ◽  
David Dogruel ◽  
Peter Williams ◽  
Russ Granzow
Keyword(s):  
Mass Spectrometry ◽  
Interaction Analysis ◽  
Biomolecular Interaction ◽  
Biomolecular Interaction Analysis

scholarly journals Detection of bound and free IGF-1 and IGF-2 in human plasma via biomolecular interaction analysis mass spectrometry

FEBS Letters ◽  
2003 ◽  
Vol 536 (1-3) ◽  
pp. 130-134 ◽  
Author(s):  
Dobrin Nedelkov ◽  
Randall W. Nelson ◽  
Urban A. Kiernan ◽  
Eric E. Niederkofler ◽  
Kemmons A. Tubbs
Keyword(s):  
Mass Spectrometry ◽  
Human Plasma ◽  
Interaction Analysis ◽  
Biomolecular Interaction ◽  
Biomolecular Interaction Analysis

scholarly journals Detection of Staphylococcal Enterotoxin B via Biomolecular Interaction Analysis Mass Spectrometry

2003 ◽  
Vol 69 (9) ◽  
pp. 5212-5215 ◽  
Author(s):  
Dobrin Nedelkov ◽  
Randall W. Nelson
Keyword(s):  
Mass Spectrometry ◽  
Limit Of Detection ◽  
Interaction Analysis ◽  
Mass Measurement ◽  
Food Samples ◽  
Biomolecular Interaction ◽  
Standard Samples ◽  
Affinity Capture ◽  
Biomolecular Interaction Analysis ◽  
Enterotoxin B

ABSTRACT Detection of Staphylococcus enterotoxin B (SEB) by biomolecular interaction analysis mass spectrometry (BIA/MS) is presented in this work. The BIA/MS experiments were based on a surface plasmon resonance (SPR) MS immunoassay that detects affinity-captured SEB both via SPR and by means of exact and direct mass measurement by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Experiments were performed with standard samples and food samples to assess the BIA/MS limit of detection for SEB and to set the experimental parameters for proper quantitation. Single and double SPR referencing was performed to accurately estimate the amount of the bound toxin. Reproducible detection of 1 ng of SEB per ml, corresponding to affinity capture and MS analysis of ∼500 amol of SEB, was readily achieved from both the standard and mushroom samples. A certain amount of SEB degradation was indicated by the signals in the mass spectra. The combination of MS with SPR-based methods of detection creates a unique approach capable of quantifying and qualitatively analyzing protein toxins from pathogenic organisms.

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