Coiled coil miniprotein randomization on phage leads to charge pattern mimicry of the receptor recognition determinant of interleukin 5

2002 ◽  
Vol 15 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Chuanzhao Li ◽  
Carmela G. Plugariu ◽  
Joanna Bajgier ◽  
John R. White ◽  
Kristin M. Liefer ◽  
...  
Keyword(s):  
Coiled Coil ◽  
Interleukin 5 ◽  
Receptor Recognition ◽  
Recognition Determinant

scholarly journals Multisite Mutagenesis of Interleukin 5 Differentiates Sites for Receptor Recognition and Receptor Activation

Biochemistry ◽  
2001 ◽  
Vol 40 (3) ◽  
pp. 852-852 ◽  
Author(s):  
Carmela G. Plugariu ◽  
Sheng-Jiun Wu ◽  
Wentao Zhang ◽  
Irwin Chaiken
Keyword(s):  
Receptor Activation ◽  
Interleukin 5 ◽  
Receptor Recognition

Multisite Mutagenesis of Interleukin 5 Differentiates Sites for Receptor Recognition and Receptor Activation†

Biochemistry ◽  
2000 ◽  
Vol 39 (48) ◽  
pp. 14939-14949 ◽  
Author(s):  
Carmela G. Plugariu ◽  
Sheng-Jiun Wu ◽  
Wentao Zhang ◽  
Irwin Chaiken
Keyword(s):  
Receptor Activation ◽  
Interleukin 5 ◽  
Receptor Recognition

TMCC3 localizes at the three-way junctions for the proper tubular network of the endoplasmic reticulum

2019 ◽  
Vol 476 (21) ◽  
pp. 3241-3260
Author(s):  
Sindhu Wisesa ◽  
Yasunori Yamamoto ◽  
Toshiaki Sakisaka
Keyword(s):  
Endoplasmic Reticulum ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Mammalian Cells ◽  
Coiled Coil ◽  
Transmembrane Domains ◽  
Domain Family ◽  
Coiled Coil Domain ◽  
U2os Cells ◽  
Er Membrane

The tubular network of the endoplasmic reticulum (ER) is formed by connecting ER tubules through three-way junctions. Two classes of the conserved ER membrane proteins, atlastins and lunapark, have been shown to reside at the three-way junctions so far and be involved in the generation and stabilization of the three-way junctions. In this study, we report TMCC3 (transmembrane and coiled-coil domain family 3), a member of the TEX28 family, as another ER membrane protein that resides at the three-way junctions in mammalian cells. When the TEX28 family members were transfected into U2OS cells, TMCC3 specifically localized at the three-way junctions in the peripheral ER. TMCC3 bound to atlastins through the C-terminal transmembrane domains. A TMCC3 mutant lacking the N-terminal coiled-coil domain abolished localization to the three-way junctions, suggesting that TMCC3 localized independently of binding to atlastins. TMCC3 knockdown caused a decrease in the number of three-way junctions and expansion of ER sheets, leading to a reduction of the tubular ER network in U2OS cells. The TMCC3 knockdown phenotype was partially rescued by the overexpression of atlastin-2, suggesting that TMCC3 knockdown would decrease the activity of atlastins. These results indicate that TMCC3 localizes at the three-way junctions for the proper tubular ER network.

Interleukin-4, interferon-gamma and interleukin-5 in peripheral blood of children with moderate atopic asthma

1997 ◽  
Vol 27 (11) ◽  
pp. 1254-1260 ◽  
Author(s):  
M. O. HOEKSTRA ◽  
Y. HOEKSTRA ◽  
D. DE REUS ◽  
B. RUTGERS ◽  
J. GERRITSEN ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Peripheral Blood ◽  
Atopic Asthma ◽  
Interleukin 4 ◽  
Interleukin 5

Inhibition of Platelet Adhesion to Fibrin(ogen) in Flowing Whole Blood by Arg-Gly-Asp and Fibrinogen γ-Chain Carboxy Terminal Peptides

1992 ◽  
Vol 68 (06) ◽  
pp. 694-700 ◽  
Author(s):  
Roy R Hantgan ◽  
Silvia C Endenburg ◽  
I Cavero ◽  
Gérard Marguerie ◽  
André Uzan ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Integrin Receptor ◽  
Shear Rates ◽  
Perfusion Chamber ◽  
Receptor Recognition ◽  
Adhesive Interactions ◽  
Coated Surfaces ◽  
Carboxy Terminal

SummaryWe have employed synthetic peptides with sequences corresponding to the integrin receptor-recognition regions of fibrinogen as inhibitors of platelet aggregation and adhesion to fibrinogen-and fibrin-coated surfaces in flowing whole blood, using a rectangular perfusion chamber at wall shear rates of 300 s–1 and 1,300 s–1. D-RGDW caused substantial inhibition of platelet aggregation and adhesion to fibrinogen and fibrin at both shear rates, although it was least effective at blocking platelet adhesion to fibrin at 300 s–1. RGDS was a weaker inhibitor, and produced a biphasic dose-response curve; SDRG was inactive. HHLGGAK-QAGDV partially inhibited platelet aggregation and adhesion to fibrin(ogen) at both shear rates. These results support the identification of an RGD-specific receptor, most likely the platelet integrin glycoprotein IIb: III a, as the primary receptor responsible for platelet: fibrin(ogen) adhesive interactions under flow conditions, and indicate that platelet adhesion to surface bound fibrin(ogen) is stabilized by multivalent receptor-ligand contacts.

Hubungan Antara Jenis Kelamin dan Riwayat Asma dengan Rinitis Alergi pada Pelajar SMP Muhammadiyah 3 Palembang

2020 ◽  
Vol 2 (1) ◽  
pp. 17
Author(s):  
Meilina Wardhani ◽  
Ressy Irma Juwita ◽  
Mitayani Purwoko
Keyword(s):  
Immunoglobulin E ◽  
Cross Sectional ◽  
Interleukin 5

Latar Belakang: Rinitis alergi adalah suatu penyakit pada hidung yang ditimbulkan oleh reaksi inflamasi mpada mukosa hidung dengan perantara immunoglobulin E. Prevalensi rinitis alergi di dunia telah meningkat termasuk di Indonesia yang kini telah mencapai 1,5-12.4% dan cenderung mengalami peningkatan setiap tahunnya. Penelitian ini bertujuan untuk mencari prevalensi rinitis alergi dan hubungan antara jenis kelamin dan riwayat asma dengan kejadian rinitis alergi pada siswa sekolah di Palembang. Metode: Penelitian ini adalah penelitian analitik observasional dengan desain cross sectional. Penelitian dilakukan di SMP Muhammadiyah 3 Palembang pada tahun 2018. Data primer diperoleh dengan cara meminta subjek penelitian untuk mengisi kuesioner ISAAC. Kriteria inklusi penelitian ini adalah siswa-siswi berusia 13-14 tahun. Besar sampel yang digunakan sebanyak 80 responden, diambil teknik total sampling.Hasil: Pada penelitian ini didapatkan prevalensi rinitis alergi sebesar 51,2% dan jenis kelamin (p=0,014) dan riwayat asma (p=0,019) sebagai faktor risiko terjadinya rinitis alergi.  Perempuan lebih banyak menderita rinitis alergi dibanding laki-laki kemungkinan disebabkan perempuan lebih sering terpapar allergen berupa debu akibat sering melakukan pekerjaan rumah tangga seperti menyapu. Adanya paparan alergen terhadap mukosa hidung akan meningkatkan konsentrasi berbagai faktor yang terkait asma seperti eosinophil, interleukin-5 dan sel CD34 di darah perifer. Kesimpulan: Jenis kelamin dan riwayat asma merupakan faktor risiko terjadinya rinitis alergi pada anak.

Sign in / Sign up

Export Citation Format

Share Document