Binding of Leishmania spp with gold nanoparticles supported polyethylene glycol and its application for the sensitive detection of infectious photogenes in human plasma samples: A novel biosensor

2020 ◽  
Vol 33 (7) ◽  
Author(s):  
Ahmad Mobed ◽  
Parina Mehri ◽  
Mohammad Hasanzadeh ◽  
Ahad Mokhtarzadeh
Keyword(s):  
Gold Nanoparticles ◽  
Polyethylene Glycol ◽  
Human Plasma ◽  
Sensitive Detection ◽  
Plasma Samples ◽  
Human Plasma Samples ◽  
Leishmania Spp

scholarly journals In Situ Amplified Chemiluminescent Detection of DNA and Immunoassay of IgG Using Special-Shaped Gold Nanoparticles as Label

2006 ◽  
Vol 52 (10) ◽  
pp. 1958-1961 ◽  
Author(s):  
Zhouping Wang ◽  
Jianqiang Hu ◽  
Yan Jin ◽  
Xin Yao ◽  
Jinghong Li
Keyword(s):  
Gold Nanoparticles ◽  
Human Plasma ◽  
Clinical Laboratory ◽  
Limit Of Detection ◽  
Dna Detection ◽  
Clinical Samples ◽  
Plasma Samples ◽  
Au Nps ◽  
Human Plasma Samples

Abstract Background: Au(III) catalyzed luminol chemiluminescence (CL) is classic in luminescence analysis. Recently, spherical gold nanoparticles (Au-NPs) were found displaying far stronger catalytic activity on luminol CL than that of Au(III). Some methods based on Au-NPs probes have been developed for DNA detection or immunoassay. However, more complicated labeling or stripping procedures are often inescapable in these protocols. Methods: We synthesized specially shaped, irregular gold nanoparticles (IGNPs) and found their catalytic efficiency on luminol CL to be 100-fold greater than that of spherical Au-NPs. Using the IGNPs-functionalized DNA oligomers and the IGNPs-modified anti-IgG as in situ chemiluminescent probes, we established sandwich-type analytic methods for rapid, simple, selective, and sensitive sequence-specific DNA detection and for human plasma IgG immunoassay, respectively. We used 12 clinical human plasma samples to examine the precision and accuracy of the proposed method for IgG content determination. Results: Calibration curves for the oligonucleotide [ΔI = 15.73 + 27.55 (DNA) × 1010 (mol/L); R2 = 0.9936] and IgG [ΔI = 48.84 + 30.23 (IgG) × 1010 (mol/L); R2 = 0.9964] show good correlation, demonstrating the linear response over the concentrations tested (0.04–10 nmol/L for DNA, 0.05–10 nmol/L for IgG). The limit of detection, calculated based on 50 μL of a solution of calibrators, was 13 pmol/L for DNA and 17 pmol/L for IgG, with a signal-to-noise ratio of 3. We obtained good intra-and interassay reproducibility. The IgG contents in 12 human plasma samples obtained by the proposed method are identical with the data of clinical laboratory. Conclusions: We developed a simple and sensitive method for in situ amplified chemiluminescence detection of sequence-specific DNA and immunoassay of IgG by use of highly active, specially shaped, irregular gold nanoparticles (IGNPs) as label and confirmed by clinical samples test. This method has many desirable features including rapid detection, selectivity, and little required instrumentation. This new protocol may be quite promising, with potentially broad applications for clinical immunoassays and DNA hybridization analysis.

scholarly journals Protocol to analyze circulating small non-coding RNAs by high-throughput RNA sequencing from human plasma samples

2021 ◽  
Vol 2 (3) ◽  
pp. 100606
Author(s):  
Giuseppina E. Grieco ◽  
Guido Sebastiani ◽  
Daniela Fignani ◽  
Noemi Brusco ◽  
Laura Nigi ◽  
...  
Keyword(s):  
Human Plasma ◽  
Rna Sequencing ◽  
High Throughput ◽  
Plasma Samples ◽  
Human Plasma Samples ◽  
Non Coding Rnas

Optimizationvia experimental design of an SPE-HPLC-UV-fluorescence method for the determination of valsartan and its metabolite in human plasma samples

2006 ◽  
Vol 29 (15) ◽  
pp. 2265-2283 ◽  
Author(s):  
Gorka Iriarte ◽  
Nerea Ferreirós ◽  
Izaskun Ibarrondo ◽  
Rosa Maria Alonso ◽  
Miren Itxaso Maguregi ◽  
...  
Keyword(s):  
Experimental Design ◽  
Human Plasma ◽  
Fluorescence Method ◽  
Plasma Samples ◽  
Uv Fluorescence ◽  
Human Plasma Samples

scholarly journals Comparison of 4-plex to 8-plex iTRAQ Quantitative Measurements of Proteins in Human Plasma Samples

2012 ◽  
Vol 11 (7) ◽  
pp. 3774-3781 ◽  
Author(s):  
Gwenael Pottiez ◽  
Jayme Wiederin ◽  
Howard S. Fox ◽  
Pawel Ciborowski
Keyword(s):  
Human Plasma ◽  
Plasma Samples ◽  
Quantitative Measurements ◽  
Human Plasma Samples

Long-Term Performance and Stability of Molecular Shotgun Lipidomic Analysis of Human Plasma Samples

2013 ◽  
Vol 85 (18) ◽  
pp. 8757-8763 ◽  
Author(s):  
Laura A. Heiskanen ◽  
Matti Suoniemi ◽  
Hung Xuan Ta ◽  
Kirill Tarasov ◽  
Kim Ekroos
Keyword(s):  
Human Plasma ◽  
Plasma Samples ◽  
Lipidomic Analysis ◽  
Human Plasma Samples ◽  
Long Term Performance ◽  
Term Performance

Validation of an UPLC-MS/MS Method for Quantitative Analysis of Raltegravir in Human Plasma Samples

2013 ◽  
Vol 35 (2) ◽  
pp. 258-263 ◽  
Author(s):  
Serena Fortuna ◽  
Enzo Ragazzoni ◽  
Lucia Lisi ◽  
Simona Di Giambenedetto ◽  
Massimiliano Fabbiani ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Human Plasma ◽  
Plasma Samples ◽  
Human Plasma Samples
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