Thermodynamic analysis of ANS binding to partially unfolded α-lactalbumin: correlation of endothermic to exothermic changeover with formation of authentic molten globules

2016 ◽  
Vol 29 (9) ◽  
pp. 446-451 ◽  
Author(s):  
Ki Hyung Kim ◽  
Soi Yun ◽  
K. H. Mok ◽  
E. K. Lee
Keyword(s):  
Thermodynamic Analysis ◽  
Ans Binding ◽  
Molten Globules ◽  
Partially Unfolded

Conformation of eukaryotic ribosomal RNAs

1983 ◽  
Vol 41 ◽  
pp. 592-593
Author(s):  
M. Boublik ◽  
G. Thornton ◽  
G. Oostergetel ◽  
J.F. Hainfeld ◽  
J.S. Wall
Keyword(s):  
Molecular Weight ◽  
Mass Measurement ◽  
Carbon Film ◽  
Structural Complexity ◽  
Scanning Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Pure Nitrogen ◽  
Freeze Dried ◽  
Scanning Transmission ◽  
Partially Unfolded

Understanding the structural complexity of ribosomes and their role in protein synthesis requires knowledge of the conformation of their components - rRNAs and proteins. Application of dedicated scanning transmission electron microscope (STEM), electrical discharge of the support carbon film in an atmosphere of pure nitrogen, and determination of the molecular weight of individual rRNAs enabled us to obtain high resolution electron microscopic images of unstained freeze-dried rRNA molecules from BHK cells in a form suitable for evaluation of their 3-D structure. Preliminary values for the molecular weight of 28S RNA from the large and 18S RNA from the small ribosomal subunits as obtained by mass measurement were 1.84 x 106 and 0.97 x 106, respectively. Conformation of rRNAs consists, in general, of alternating segments of intramolecular hairpin stems and single stranded loops in a proportion which depends on their ionic environment, the Mg++ concentration in particular. Molecules of 28S RNA (Fig. 1) and 18S RNA (not shown) obtained by freeze-drying from a solution of 60 mM NH+4 acetate and 2 mM Mg++ acetate, pH 7, appear as partially unfolded coils with compact cores suggesting a high degree of ordered secondary structure.

Thermodynamic analysis of binding of p-substituted benzamidines to trypsin

2001 ◽  
Vol 268 (6) ◽  
pp. 1554-1560 ◽  
Author(s):  
Reinskje Talhout ◽  
Jan B. F. N. Engberts
Keyword(s):  
Thermodynamic Analysis

A Thermodynamic Analysis of Three and Four-Wheel Air Cycle Machines for Aeronautical Applications

2015 ◽  
Vol 9 (2) ◽  
pp. 190
Author(s):  
Meiriele A. Alvarenga ◽  
Claudia R. Andrade ◽  
Edson L. Zaparoli
Keyword(s):  
Thermodynamic Analysis
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