A new paradigm in molecular recognition? specific antibody binding to membrane-inserted HIV-1 epitopes

2011 ◽  
Vol 24 (4) ◽  
pp. 642-646 ◽  
Author(s):  
José L. Nieva ◽  
Beatriz Apellaniz ◽  
Nerea Huarte ◽  
Maier Lorizate
Keyword(s):  
Molecular Recognition ◽  
Specific Antibody ◽  
Antibody Binding ◽  
New Paradigm ◽  
Hiv 1

scholarly journals Differences in the Binding Affinity of an HIV-1 V2 Apex-Specific Antibody for the SIVsmm/macEnvelope Glycoprotein Uncouple Antibody-Dependent Cellular Cytotoxicity from Neutralization

mBio ◽  
2019 ◽  
Vol 10 (4) ◽  
Author(s):  
Benjamin von Bredow ◽  
Raiees Andrabi ◽  
Michael Grunst ◽  
Andres G. Grandea ◽  
Khoa Le ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Specific Antibody ◽  
Neutralizing Antibody ◽  
Glycosylation Site ◽  
Antibody Binding ◽  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Broadly Neutralizing Antibody ◽  
Infected Cells ◽  
Hiv 1

ABSTRACTAs a consequence of their independent evolutionary origins in apes and Old World monkeys, human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency viruses of the SIVsmm/maclineage express phylogenetically and antigenically distinct envelope glycoproteins. Thus, HIV-1 Env-specific antibodies do not typically cross-react with the Env proteins of SIVsmm/macisolates. Here we show that PGT145, a broadly neutralizing antibody to a quaternary epitope at the V2 apex of HIV-1 Env, directs the lysis of SIVsmm/mac-infected cells by antibody-dependent cellular cytotoxicity (ADCC) but does not neutralize SIVsmm/macinfectivity. Amino acid substitutions in the V2 loop of SIVmac239 corresponding to the epitope for PGT145 in HIV-1 Env modulate sensitivity to this antibody. Whereas a substitution in a conserved N-linked glycosylation site (N171Q) eliminates sensitivity to ADCC, a lysine-to-serine substitution in this region (K180S) increases ADCC and renders the virus susceptible to neutralization. These differences in function correlate with an increase in the affinity of PGT145 binding to Env on the surface of virus-infected cells and to soluble Env trimers. To our knowledge, this represents the first instance of an HIV-1 Env-specific antibody that cross-reacts with SIVsmm/macEnv and illustrates how differences in antibody binding affinity for Env can differentiate sensitivity to ADCC from neutralization.IMPORTANCEHere we show that PGT145, a potent broadly neutralizing antibody to HIV-1, directs the lysis of SIV-infected cells by antibody-dependent cellular cytotoxicity but does not neutralize SIV infectivity. This represents the first instance of cross-reactivity of an HIV-1 Env-specific antibody with SIVsmm/macEnv and reveals that antibody binding affinity can differentiate sensitivity to ADCC from neutralization.

Epitope scanning reveals gain and loss of strain specific antibody binding epitopes associated with the conversion of normal cellular prion to scrapie prion

2004 ◽  
Vol 90 (5) ◽  
pp. 1205-1217 ◽  
Author(s):  
Tao Pan ◽  
Ruliang Li ◽  
Shin-Cheng Kang ◽  
Boon-Seng Wong ◽  
Thomas Wisniewski ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Antibody Binding ◽  
Gain And Loss

Interactions of human antibodies, epitope exposure, antibody binding and neutralization of primary isolate HIV-1 virions

AIDS ◽  
2002 ◽  
Vol 16 (18) ◽  
pp. 2409-2417 ◽  
Author(s):  
Lisa A Cavacini ◽  
Mark Duval ◽  
James Robinson ◽  
Marshall R Posner
Keyword(s):  
Antibody Binding ◽  
Primary Isolate ◽  
Human Antibodies ◽  
Hiv 1

Screening and expressing HIV-1 specific antibody fragments in Saccharomyces cerevisiae

2018 ◽  
Vol 103 ◽  
pp. 279-285 ◽  
Author(s):  
Ying Wang ◽  
Yaming Shan ◽  
Xinyu Gao ◽  
Rui Gong ◽  
Jun Zheng ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Specific Antibody ◽  
Antibody Fragments ◽  
Hiv 1

Technical report: xMAPr – High-dynamic-range (HDR) quantification of antigen-specific antibody binding

2020 ◽  
Vol 212 ◽  
pp. 103577 ◽  
Author(s):  
Tanja C. Meyer ◽  
Frank Schmidt ◽  
Jörn Steinke ◽  
Barbara M. Bröker ◽  
Uwe Völker ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Dynamic Range ◽  
High Dynamic Range ◽  
Antibody Binding ◽  
Technical Report ◽  
High Dynamic

A possible mechanism of molecular recognition for the reverse transcriptase of HIV-1

1992 ◽  
Vol 44 (S19) ◽  
pp. 225-253 ◽  
Author(s):  
Joaquim Delphino Da Motta Neto ◽  
Michael Charles Zerner ◽  
Ricardo Bicca De Alencastro
Keyword(s):  
Molecular Recognition ◽  
Reverse Transcriptase ◽  
Hiv 1

Molecular recognition in the HIV-1 capsid/cyclophilin A complex 1 1Edited by J. A. Wells

1997 ◽  
Vol 269 (5) ◽  
pp. 780-795 ◽  
Author(s):  
Sanghee Yoo ◽  
David G Myszka ◽  
Chin-yah Yeh ◽  
Maureen McMurray ◽  
Christopher P Hill ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Cyclophilin A ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document