Three-dimensional virtual histology of silurian osteostracan scales revealed by synchrotron radiation microtomography

2015 ◽  
Vol 276 (8) ◽  
pp. 873-888 ◽  
Author(s):  
Qingming Qu ◽  
Henning Blom ◽  
Sophie Sanchez ◽  
Per Ahlberg
Keyword(s):  
Synchrotron Radiation ◽  
Three Dimensional ◽  
Virtual Histology ◽  
Synchrotron Radiation Microtomography

Nondestructive imaging of the internal microstructure of vessels and nerve fibers in rat spinal cord using phase-contrast synchrotron radiation microtomography

2017 ◽  
Vol 24 (2) ◽  
pp. 482-489 ◽  
Author(s):  
Jianzhong Hu ◽  
Ping Li ◽  
Xianzhen Yin ◽  
Tianding Wu ◽  
Yong Cao ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Nerve Fibers ◽  
The Body ◽  
Rat Spinal Cord ◽  
Nondestructive Imaging ◽  
Synchrotron Radiation Microtomography ◽  
The Brain

The spinal cord is the primary neurological link between the brain and other parts of the body, but unlike those of the brain, advances in spinal cord imaging have been challenged by the more complicated and inhomogeneous anatomy of the spine. Fortunately with the advancement of high technology, phase-contrast synchrotron radiation microtomography has become widespread in scientific research because of its ability to generate high-quality and high-resolution images. In this study, this method has been employed for nondestructive imaging of the internal microstructure of rat spinal cord. Furthermore, digital virtual slices based on phase-contrast synchrotron radiation were compared with conventional histological sections. The three-dimensional internal microstructure of the intramedullary arteries and nerve fibers was vividly detected within the same spinal cord specimen without the application of a stain or contrast agent or sectioning. With the aid of image post-processing, an optimization of vessel and nerve fiber images was obtained. The findings indicated that phase-contrast synchrotron radiation microtomography is unique in the field of three-dimensional imaging and sets novel standards for pathophysiological investigations in various neurovascular diseases.

scholarly journals Three-Dimensional Neuronal Structure of Human Cerebral Cortex Determined by Synchrotron-Radiation Microtomography

2015 ◽  
Vol 21 (S3) ◽  
pp. 919-920
Author(s):  
Rino Saiga ◽  
Susumu Takekoshi ◽  
Chie Inomoto ◽  
Naoya Nakamura ◽  
Akio Tsuboi ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Synchrotron Radiation ◽  
Three Dimensional ◽  
Neuronal Structure ◽  
Human Cerebral Cortex ◽  
Synchrotron Radiation Microtomography

Three-dimensional bone morphometry using synchrotron radiation microtomography

Bone ◽  
1996 ◽  
Vol 19 (3) ◽  
pp. 158
Author(s):  
M. Pateyron ◽  
F. Peyrin ◽  
A.M. Laval-Jeantet ◽  
P. Cloetens ◽  
E. Cendre
Keyword(s):  
Synchrotron Radiation ◽  
Three Dimensional ◽  
Bone Morphometry ◽  
Synchrotron Radiation Microtomography

Three-dimensional characterization of the microstructure in rabbit patella–patellar tendon interface using propagation phase-contrast synchrotron radiation microtomography

2018 ◽  
Vol 25 (6) ◽  
pp. 1833-1840 ◽  
Author(s):  
Yongchun Zhou ◽  
Jianzhong Hu ◽  
Jingyong Zhou ◽  
Ziteng Zeng ◽  
Yong Cao ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Patellar Tendon ◽  
Phase Contrast ◽  
Phase Retrieval ◽  
Three Dimensional ◽  
Histological Section ◽  
Therapeutic Interventions ◽  
Safranin O ◽  
Synchrotron Radiation Microtomography

Understanding the three-dimensional ultrastructure morphology of tendon-to-bone interface may allow the development of effective therapeutic interventions for enhanced interface healing. This study aims to assess the feasibility of propagation phase-contrast synchrotron radiation microtomography (PPC-SRµCT) for three-dimensional characterization of the microstructure in rabbit patella–patellar tendon interface (PPTI). Based on phase retrieval for PPC-SRµCT imaging, this technique is capable of visualizing the three-dimensional internal architecture of PPTI at a cellular high spatial resolution including bone and tendon, especially the chondrocytes lacuna at the fibrocartilage layer. The features on the PPC-SRµCT image of the PPTI are similar to those of a histological section using Safranin-O staining/fast green staining. The three-dimensional microstructure in the rabbit patella–patellar tendon interface and the spatial distributions of the chondrocytes lacuna and their quantification volumetric data are displayed. Furthermore, a color-coding map differentiating cell lacuna in terms of connecting beads is presented after the chondrocytes cell lacuna was extracted. This provides a more in-depth insight into the microstructure of the PPTI on a new scale, particularly the cell lacuna arrangement at the fibrocartilage layer. PPC-SRµCT techniques provide important complementary information to the conventional histological method for characterizing the microstructure of the PPTI, and may facilitate in investigations of the repair mechanism of the PPTI after injury and in evaluating the efficacy of a different therapy.

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