Selective responses (actin polymerization, shape changes, locomotion, pinocytosis) to the PKC inhibitor Ro 31-8220 suggest that PKC discriminately regulates functions of human blood lymphocytes

1995 ◽  
Vol 57 (4) ◽  
pp. 587-591 ◽  
Author(s):  
S. Trachsel ◽  
H. U. Keller
Keyword(s):  
Human Blood ◽  
Actin Polymerization ◽  
Pkc Inhibitor ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes ◽  
Shape Changes

Study of interaction between l-glutamate and human blood lymphocytes

1997 ◽  
Vol 58 (3) ◽  
pp. 177-180 ◽  
Author(s):  
I.A Kostanyan ◽  
M.I Merkulova ◽  
E.V Navolotskaya ◽  
R.I Nurieva
Keyword(s):  
Human Blood ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes

A simplified and reliable assay for complex I in human blood lymphocytes

2007 ◽  
Vol 326 (1-2) ◽  
pp. 76-82 ◽  
Author(s):  
L.E.A. de Wit ◽  
L. Spruijt ◽  
G.C. Schoonderwoerd ◽  
I.F.M. de Coo ◽  
H.J.M. Smeets ◽  
...  
Keyword(s):  
Human Blood ◽  
Complex I ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes

Modulation of UVB-induced Oxidative Stress by Ursolic Acid in Human Blood Lymphocytes

2007 ◽  
Vol 3 (1) ◽  
pp. 11-18 ◽  
Author(s):  
S. Ramachandr ◽  
N. Rajendra Prasad ◽  
K.V. Pugalendi ◽  
Venugopal P. Menon
Keyword(s):  
Oxidative Stress ◽  
Ursolic Acid ◽  
Human Blood ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes

Potential of rod, sphere and semi-cube shaped gold nanoparticles to induce cytotoxicity and genotoxicity in human blood lymphocytes in vitro

2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Mona A.M. Abo-Zeid ◽  
Thomas Liehr ◽  
Amira M. Gamal-Eldeen ◽  
Mahmoud Zawrah ◽  
Mostafa Ali ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Gold Nanoparticles ◽  
Mitotic Activity ◽  
Human Blood ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes ◽  
Different Shapes

AbstractGold nanoparticles (GNPs) are intended to be used in nanomedicine. Due to nanotechnology innovation GNPs of variable sizes and in different shapes including rods, spheres, cubes, etc., can easily be produced. The aim of the present studies was to evaluate the cyto-and genotoxicity inducible by different shaped GNPs on normal human peripheral blood lymphocytes.Four different shapes of GNPs including big rod GNPs (BR-GNPs, 50 nm), small rod GNPs (SR-GNPs, 30 nm), sphere GNPs (S-GNPs, 15 nm) and semi-cube GNPs (SC-GNPs, 15 nm) were studied. Cultured human blood lymphocytes were treated with different concentrations of these GNPs for 24 h in vitro. Cytotoxicity was evaluated based on the mitotic index (MI), while genotoxicity was studied by an interphase-fluorescence in situ hybridization (I-FISH) assay. The following genes were studied in I-FISH:The lowest concentration of BR-GNPs neither had an effect mitotic activity nor enhanced gain or loss of examined gene signals in a significant manner with I-FSH. Other concentrations of BR-GNPs, SR-GNPs, S-GNPs and SC-GNPs with all concentrations inhibited the mitotic activity of the cells and reduced the cell proliferation highly significantly. The different types of GNPs initiated the duplication ofGNPs at high concentration can reduce the cell proliferation and induce DNA damage. Low concentration of rod-shaped GNPs at 50 nm was safe on human lymphocytes. Further research studies are required to optimize the concentration, shape and size of GNPs before using them in nanomedicine.

Diacylglycerols and PMA induce actin polymerization and distinct shape changes in lymphocytes: relation to fluid pinocytosis and locomotion

1989 ◽  
Vol 93 (3) ◽  
pp. 457-465
Author(s):  
H.U. Keller ◽  
V. Niggli ◽  
A. Zimmermann
Keyword(s):  
Actin Polymerization ◽  
Shape Change ◽  
Second Messengers ◽  
Cell Periphery ◽  
Response Curves ◽  
Blood Lymphocytes ◽  
Different Types ◽  
Net Uptake ◽  
Very High ◽  
Shape Changes

Shape changes have been determined in human blood lymphocytes stimulated with OAG, diC8, PMA, colchicine or the hexapeptide fNLPNTL in short-term assays (30 min). Distinct types of shape-change responses were observed. Colchicine was active in generating a relatively small proportion of polarized lymphocytes (front-tail polarity). OAG, diC8 and PMA produced different types of shape change (non-polar cells with surface projections), and these were closely associated with an increase in actin polymerization and a shift of F-actin into the projections at the cell periphery. The diacylglycerols OAG and diC8 produced biphasic dose-response curves leading to rounding up of cells at very high stimulant concentrations. PMA produced no comparable biphasic response when tested over a much wider concentration range. Though the nonpolar cells with surface projections generated by OAG, diC8 or PMA showed vigorous shape changes, they lacked significant locomotor activity. alpha-Phorbol, 4 alpha-PDD, lumicolchicine or fNLPNTL were inactive. Small blood lymphocytes stimulated by OAG, diC8 or PMA showed a very small increase in the net uptake of FITC-dextran by fluid pinocytosis. Unlike neutrophils, which show a high net uptake, lymphocytes did not concentrate FITC-dextran in large granules, indicating that they do not develop a ‘storage’ compartment in the form of large vesicles. However, small fluorescent spots were consistently found in at least a fraction of blood lymphocytes. The results indicate that stimulated surface movement may be instrumental in fluid pinocytosis. Diacylglycerols may act as second messengers to induce pinocytosis, shape changes and altered actin polymerization in lymphocytes.

Toxicity of Fe 2 O 3 nanoparticles on human blood lymphocytes

2019 ◽  
Vol 33 (6) ◽  
Author(s):  
Evelyn Assadian ◽  
Hamid Dezhampanah ◽  
Enayatollah Seydi ◽  
Jalal Pourahmad
Keyword(s):  
Human Blood ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes
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