Stimulation of human polymorphonuclear leukocytes by leukotriene B4 and platelet-activating factor: an ultrastructural and pharmacological study

1993 ◽  
Vol 53 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Adriano G. Rossi ◽  
D. Euan MacIntyre ◽  
Carolyn J.P. Jones ◽  
Rodger M. McMillan
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Pharmacological Study ◽  
Platelet Activating Factor ◽  
Leukotriene B4 ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

Interactions between synthesis of platelet-activating factor and leukotriene B4 in isolated human polymorphonuclear leukocytes

Inflammation ◽  
1993 ◽  
Vol 17 (6) ◽  
pp. 705-714 ◽  
Author(s):  
E. Moilanen ◽  
A. -L. Kirkkola ◽  
H. Kankaanranta ◽  
M. M. Nieminen ◽  
H. Vapaatalo
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Platelet Activating Factor ◽  
Leukotriene B4 ◽  
Human Polymorphonuclear Leukocytes

scholarly journals The mechanism of leukotriene B4 export from human polymorphonuclear leukocytes.

1990 ◽  
Vol 265 (23) ◽  
pp. 13438-13441
Author(s):  
B.K. Lam ◽  
L. Gagnon ◽  
K.F. Austen ◽  
R.J. Soberman
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Leukotriene B4 ◽  
Human Polymorphonuclear Leukocytes

scholarly journals Pertussis toxin inhibition of chemotactic factor-induced calcium mobilization and function in human polymorphonuclear leukocytes.

1985 ◽  
Vol 162 (1) ◽  
pp. 145-156 ◽  
Author(s):  
D W Goldman ◽  
F H Chang ◽  
L A Gifford ◽  
E J Goetzl ◽  
H R Bourne
Keyword(s):  
Pertussis Toxin ◽  
Lysosomal Enzyme ◽  
Polymorphonuclear Leukocytes ◽  
Regulatory Protein ◽  
Leukotriene B4 ◽  
Ionophore A23187 ◽  
Enzyme Release ◽  
Adp Ribosylation ◽  
Chemotactic Factors ◽  
Human Polymorphonuclear Leukocytes

Chemotactic factors stimulate a rapid increase in the cytosolic concentration of intracellular calcium ions ([Ca2+]in) in human polymorphonuclear leukocytes (PMNL), which may be an event that is critical to the expression of chemotaxis and other PMNL functions. Treatment of PMNL with pertussis toxin catalyzes ADP-ribosylation of a protein similar or identical to the inhibiting regulatory protein of adenylate cyclase, Gi, and suppresses the increase in [Ca2+]in elicited by leukotriene B4(LTB4) and formyl-methionyl-leucyl-phenylalanine. Chemotactic migration and lysosomal enzyme release elicited by chemotactic factors were inhibited by pertussis toxin with a concentration-dependence similar to that for inhibition of the increase in [Ca2+]in, without an effect on lysosomal enzyme release induced by the ionophore A23187 and phorbol myristate acetate. Activated pertussis toxin catalyzed the [32P]ADP-ribosylation of a 41 kD protein in homogenates of PMNL. The extent of [32P]ADP-ribosylation of this protein was reduced 59% by pretreatment of intact PMNL with pertussis toxin. Pertussis toxin selectively decreased the number of high-affinity receptors for LTB4 on PMNL by 60% without altering the number or binding properties of the low-affinity subset of receptors. Pertussis toxin modification of a membrane protein of PMNL analogous to Gi thus simultaneously alters chemotactic receptors and attenuates the changes in cytosolic calcium concentration and PMNL function caused by chemotactic factors.

Human polymorphonuclear leukocytes release leukotriene B4 during phagocytosis ofStaphylococcus aureus

Inflammation ◽  
1986 ◽  
Vol 10 (1) ◽  
pp. 37-47 ◽  
Author(s):  
P. A. J. Henricks ◽  
M. E. Van Der Tol ◽  
F. Engels ◽  
F. P. Nijkamp ◽  
J. Verhoef
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Leukotriene B4 ◽  
Ofstaphylococcus Aureus ◽  
Human Polymorphonuclear Leukocytes
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