Characteristics of Bovine Alveolar Macrophage Elastase

1984 ◽  
Vol 35 (5) ◽  
pp. 449-457 ◽  
Author(s):  
Rudolph Valentine ◽  
Gerald L. Fisher
Keyword(s):  
Alveolar Macrophage ◽  
Bovine Alveolar Macrophage

Differential and strain-specific triggering of bovine alveolar macrophage effector functions by mycoplasmas

1996 ◽  
Vol 21 (6) ◽  
pp. 487-498 ◽  
Author(s):  
T.W. Jungi ◽  
M. Krampe ◽  
M. Sileghem ◽  
Ch. Griot ◽  
J. Nicolet
Keyword(s):  
Alveolar Macrophage ◽  
Effector Functions ◽  
Bovine Alveolar Macrophage

Endotoxin-mediated bovine alveolar macrophage procoagulant induction is dependent on protein kinase C activation

Inflammation ◽  
1990 ◽  
Vol 14 (6) ◽  
pp. 681-689 ◽  
Author(s):  
Bruce D. Car ◽  
David O. Slauson ◽  
Monique Dor� ◽  
M. Mitsu Suyemoto
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Alveolar Macrophage ◽  
Kinase C ◽  
Bovine Alveolar Macrophage ◽  
Protein Kinase C Activation

scholarly journals The bovine alveolar macrophage DNA methylome is resilient to infection with Mycobacterium bovis

2017 ◽  
Author(s):  
Alan Mark O’Doherty ◽  
Kevin Rue-Albrecht ◽  
David Andrew Magee ◽  
Simone Ahting ◽  
Rachelle Elizabeth Irwin ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Alveolar Macrophage ◽  
Mycobacterium Bovis ◽  
Expression Patterns ◽  
Interaction Model ◽  
Proximal Promoter ◽  
Dna Methylome ◽  
Targeted Analysis ◽  
Bovine Alveolar Macrophage ◽  
The Impact

AbstractDNA methylation is pivotal in orchestrating gene expression patterns in various mammalian biological processes. Perturbation of the bovine alveolar macrophage (bAM) transcriptome, due to Mycobacterium bovis (M. bovis) infection, has been well documented; however, the impact of this intracellular pathogen on the bAM epigenome has not been determined. Here, whole genome bisulfite sequencing (WGBS) was used to assess the effect of M. bovis infection on the bAM DNA methylome. The methylomes of bAM infected with M. bovis were compared to those of non-infected bAM 24 hours post-infection (hpi). No differences in DNA methylation (CpG or non-CpG) were observed. Analysis of DNA methylation at proximal promoter regions uncovered >250 genes harbouring intermediately methylated (IM) promoters (average methylation of 33–66%). Gene ontology analysis, focusing on genes with low, intermediate or highly methylated promoters, revealed that genes with IM promoters were enriched for immune-related GO categories; this enrichment was not observed for genes in the high or low methylation groups. Targeted analysis of genes in the IM category confirmed the WGBS observation. This study is the first in cattle examining genome-wide DNA methylation at single nucleotide resolution in an important bovine cellular host-pathogen interaction model, providing evidence for IM promoter methylation in bAM.

scholarly journals The bovine alveolar macrophage DNA methylome is resilient to infection with Mycobacterium bovis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alan Mark O’Doherty ◽  
Kevin Christophe Rue-Albrecht ◽  
David Andrew Magee ◽  
Simone Ahting ◽  
Rachelle Elizabeth Irwin ◽  
...  
Keyword(s):  
Alveolar Macrophage ◽  
Mycobacterium Bovis ◽  
Dna Methylome ◽  
Bovine Alveolar Macrophage

Effects of 4-ipomeanol on bovine alveolar macrophage function

1997 ◽  
Vol 58 (2) ◽  
pp. 133-145 ◽  
Author(s):  
Xiantang Li ◽  
Charles J. Czuprynski ◽  
William L. Castleman
Keyword(s):  
Alveolar Macrophage ◽  
Macrophage Function ◽  
Bovine Alveolar Macrophage

scholarly journals Characterization of Mannheimia (Pasteurella) haemolytica leukotoxin interaction with bovine alveolar macrophage β2 integrins

2005 ◽  
Vol 36 (5-6) ◽  
pp. 771-786 ◽  
Author(s):  
Praveen Thumbikat ◽  
Thamotharampillai Dileepan ◽  
Mathur S. Kannan ◽  
Samuel K. Maheswaran
Keyword(s):  
Alveolar Macrophage ◽  
Pasteurella Haemolytica ◽  
Bovine Alveolar Macrophage ◽  
Β2 Integrins

The bovine alveolar macrophage. 1. Isolation, in vitro cultivation, ultrastructure, and phagocytosis

1973 ◽  
Vol 19 (10) ◽  
pp. 1207-1210 ◽  
Author(s):  
M. L. Fox
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Alveolar Macrophage ◽  
Calf Serum ◽  
In Vitro Cultivation ◽  
Foetal Calf Serum ◽  
Isolated Cells ◽  
Large Numbers ◽  
Bovine Alveolar Macrophage

The bovine alveolar macrophage (BAM) was isolated in large numbers from lung washings and could be cultivated in vitro up to 63 days in medium 199, 20% foetal calf serum (FCS). Freshly isolated cells examined by electron microscopy (EM) were similar to those of the mouse. Cultured BAM prepared for EM were larger than fresh cells and contained discrete packets of homogenous material, possibly lipid. The BAM phagocytosed Staphylococcus aureus (FDA209P, type 42D) but the percentage of cells phagocytosing was reduced when FCS was not present, when cells were infected for 30 min compared with 45 min and when 2 × 107 CFU/ml compared with 2 × 108 CFU/ml were used to infect the cells.

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