scholarly journals The use of atmospheric and room temperature plasma mutagenesis to create a brewing yeast with reduced acetaldehyde production

2018 ◽  
Vol 124 (3) ◽  
pp. 236-243 ◽  
Author(s):  
Chunfeng Liu ◽  
Qi Li ◽  
Chengtuo Niu ◽  
Yaping Tian ◽  
Yijin Zhao ◽  
...  
Keyword(s):  
Room Temperature ◽  
Temperature Plasma ◽  
Brewing Yeast ◽  
Acetaldehyde Production

Reduction of Methanol in Brewed Wine by the Use of Atmospheric and Room-Temperature Plasma Method and the Combination Optimization of Malt with Different Adjuncts

2014 ◽  
Vol 79 (11) ◽  
pp. M2308-M2314 ◽  
Author(s):  
Ming-Hua Liang ◽  
Ying-Jie Liang ◽  
Jiang-Yan Chai ◽  
Shi-Shui Zhou ◽  
Jian-Guo Jiang
Keyword(s):  
Room Temperature ◽  
Temperature Plasma ◽  
Plasma Method ◽  
Combination Optimization

scholarly journals The proteins cleaved by endogenous tryptic proteases in normal EDTA plasma by C18 collection of peptides for liquid chromatography micro electrospray ionization and tandem mass spectrometry

2021 ◽  
Author(s):  
Jaimie Dufresne ◽  
Angelique Florentinus-Mefailoski ◽  
Juliet Ajambo ◽  
Ammara Ferwa ◽  
Peter Bowden ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Electrospray Ionization ◽  
Room Temperature ◽  
Cellular Proteins ◽  
Tandem Mass ◽  
Temperature Plasma ◽  
Tryptic Peptides ◽  
Gene Symbols

The tryptic peptides from ice cold versus room temperature plasma were identified by C18 liquid chromatography and micro electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS). Samples collected on ice showed low levels of endogenous tryptic peptides compared to the same samples incubated at room temperature. Plasma on ice contained peptides from albumin, complement, and apolipoproteins and others that were observed by the X!TANDEM and SEQUEST algorithms. In contrast to ice cold samples, after incubation at room temperature, greater numbers of tryptic peptides from well characterized plasma proteins, and from cellular proteins were observed. A total of 583,927 precursor ions and MS/MS spectra were correlated to 94,669 best fit peptides that reduced to 22,287 correlations to the best accession within a gene symbol and to 7174 correlations to at least 510 gene symbols with ≥ 5 independent MS/MS correlations (peptide counts) that showed FDR q-values ranging from E−9 (i.e. FDR = 0.000000001) to E−227. A set of 528 gene symbols identified by X!TANDEM and SEQUEST including C4B showed ≥ fivefold variation between ice cold versus room temperature incubation. STRING analysis of the protein gene symbols observed from endogenous peptides in normal plasma revealed an extensive protein-interaction network of cellular factors associated with cell signalling and regulation, the formation of membrane bound organelles, cellular exosomes and exocytosis network proteins. Taken together the results indicated that a pool of cellular proteins, or protein complexes, in plasma are apparently not stable and degrade soon after incubation at room temperature.

Sign in / Sign up

Export Citation Format

Share Document