scholarly journals Effects of nitrogen catabolite repression-related amino acids on the flavour of rice wine

2015 ◽  
Vol 121 (4) ◽  
pp. 581-588 ◽  
Author(s):  
Xinrui Zhao ◽  
Huijun Zou ◽  
Guocheng Du ◽  
Jian Chen ◽  
Jingwen Zhou
Keyword(s):  
Amino Acids ◽  
Catabolite Repression ◽  
Rice Wine ◽  
Nitrogen Catabolite Repression

scholarly journals Metabolic Engineering of the Regulators in Nitrogen Catabolite Repression To Reduce the Production of Ethyl Carbamate in a Model Rice Wine System

2013 ◽  
Vol 80 (1) ◽  
pp. 392-398 ◽  
Author(s):  
Xinrui Zhao ◽  
Huijun Zou ◽  
Jianwei Fu ◽  
Jingwen Zhou ◽  
Guocheng Du ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Nuclear Localization ◽  
Catabolite Repression ◽  
Genetically Modified ◽  
Nitrogen Sources ◽  
Ethyl Carbamate ◽  
Rice Wine ◽  
Nitrogen Catabolite Repression ◽  
Wine Production ◽  
Content Type

ABSTRACTRice wine has been one of the most popular traditional alcoholic drinks in China. However, the presence of potentially carcinogenic ethyl carbamate (EC) in rice wine has raised a series of food safety issues. During rice wine production, the key reason for EC formation is urea accumulation, which occurs because of nitrogen catabolite repression (NCR) inSaccharomyces cerevisiae. NCR represses urea utilization by retaining Gln3p in the cytoplasm when preferred nitrogen sources are present. In order to increase the nuclear localization of Gln3p, some possible phosphorylation sites on the nuclear localization signal were mutated and the nuclear localization regulation signal was truncated, and the disruption ofURE2provided an additional method of reducing urea accumulation. By combining these strategies, the genes involved in urea utilization (DUR1,2andDUR3) could be significantly activated in the presence of glutamine. During shake flask fermentations of the genetically modified strains, very little urea accumulated in the medium. Furthermore, the concentrations of urea and EC were reduced by 63% and 72%, respectively, in a model rice wine system. Examination of the normal nutrients in rice wine indicated that there were few differences in fermentation characteristics between the wild-type strain and the genetically modified strain. These results show that metabolic engineering of the NCR regulators has great potential as a method for eliminating EC during rice wine production.

Effect of cyclic AMP on catabolite-repressed zoosporangial formation in Phytophthora capsici

1977 ◽  
Vol 55 (7) ◽  
pp. 840-843 ◽  
Author(s):  
M. Yoshikawa ◽  
H. Masago
Keyword(s):  
Amino Acids ◽  
Citric Acid ◽  
Cyclic Amp ◽  
Catabolite Repression ◽  
Citric Acid Cycle ◽  
Phytophthora Capsici ◽  
Antimycin A ◽  
Acid Cycle

Zoosporangial formation in Phytophthora capsici was sensitively inhibited by glucose and other catabolites including sugars, citric acid cycle acids, and amino acids, but was only slightly inhibited by 3-O-methylglucose and 2-deoxyglucose and by other seemingly weak catabolites. The inhibitions were specifically prevented by cyclic AMP among the various related nucleotides evaluated. The reversing effect by cyclic AMP was observed only on zoosporangial formation that was partially repressed by catabolites, but the completely repressed zoosporangial formation could not be reversed by cyclic AMP. Furthermore, cyclic AMP failed in reversing zoosporangial formation that was inhibited by antibiotics such as antimycin A and cycloheximide. The results suggested that the initiation of zoosporangial formation in the fungus is under the control of catabolite repression that is mediated by cyclic AMP.

scholarly journals Genes of Different Catabolic Pathways Are Coordinately Regulated by Dal81 in Saccharomyces cerevisiae

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Marcos D. Palavecino ◽  
Susana R. Correa-García ◽  
Mariana Bermúdez-Moretti
Keyword(s):  
Catabolite Repression ◽  
Transcriptional Control ◽  
Nitrogen Limitation ◽  
Temporal Order ◽  
Nitrogen Sources ◽  
Nitrogen Utilization ◽  
Aminobutyric Acid ◽  
General Mechanism ◽  
Nitrogen Catabolite Repression ◽  
Catabolic Enzymes

Yeast can use a wide variety of nitrogen compounds. However, the ability to synthesize enzymes and permeases for catabolism of poor nitrogen sources is limited in the presence of a rich one. This general mechanism of transcriptional control is called nitrogen catabolite repression. Poor nitrogen sources, such as leucine, γ-aminobutyric acid (GABA), and allantoin, enable growth after the synthesis of pathway-specific catabolic enzymes and permeases. This synthesis occurs only under conditions of nitrogen limitation and in the presence of a pathway-specific signal. In this work we studied the temporal order in the induction of AGP1, BAP2, UGA4, and DAL7, genes that are involved in the catabolism and use of leucine, GABA, and allantoin, three poor nitrogen sources. We found that when these amino acids are available, cells will express AGP1 and BAP2 in the first place, then DAL7, and at last UGA4. Dal81, a general positive regulator of genes involved in nitrogen utilization related to the metabolisms of GABA, leucine, and allantoin, plays a central role in this coordinated regulation.

scholarly journals Nuclear Gln3 Import Is Regulated by Nitrogen Catabolite Repression Whereas Export Is Specifically Regulated by Glutamine

Genetics ◽  
2015 ◽  
Vol 201 (3) ◽  
pp. 989-1016 ◽  
Author(s):  
Rajendra Rai ◽  
Jennifer J. Tate ◽  
Karthik Shanmuganatham ◽  
Martha M. Howe ◽  
David Nelson ◽  
...  
Keyword(s):  
Catabolite Repression ◽  
Nitrogen Catabolite Repression
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