Effective three‐dimensional culture of hepatocyte‐like cells generated from human adipose‐derived mesenchymal stem cells

2021 ◽  
Author(s):  
Yu Saito ◽  
Tetsuya Ikemoto ◽  
Kazunori Tokuda ◽  
Katsuki Miyazaki ◽  
Shinichiro Yamada ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Three Dimensional Culture

scholarly journals Substrate-independent immunomodulatory characteristics of mesenchymal stem cells in three-dimensional culture

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0206811 ◽  
Author(s):  
Jing Li ◽  
Tong Chen ◽  
Xiahe Huang ◽  
Yunshan Zhao ◽  
Bin Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Three Dimensional Culture ◽  
Substrate Independent

scholarly journals Cartilage Matrix Formation by Bovine Mesenchymal Stem Cells in Three-dimensional Culture Is Age-dependent

2011 ◽  
Vol 469 (10) ◽  
pp. 2744-2753 ◽  
Author(s):  
Isaac E. Erickson ◽  
Steven C. van Veen ◽  
Swarnali Sengupta ◽  
Sydney R. Kestle ◽  
Robert L. Mauck
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Cartilage Matrix ◽  
Age Dependent ◽  
Three Dimensional Culture

Three dimensional culture of tooth-derived mesenchymal stem cells on poly(aspartic acid) (PASP) based hydrogel scaffolds

Pancreatology ◽  
2017 ◽  
Vol 17 (3) ◽  
pp. S40
Author(s):  
Orsolya Hegedűs ◽  
Dávid Juriga ◽  
Krisztina Nagy ◽  
Angéla Jedlovszky-Hajdú ◽  
Miklós Zrínyi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Aspartic Acid ◽  
Three Dimensional ◽  
Hydrogel Scaffolds ◽  
Three Dimensional Culture

scholarly journals Paracrine study of adipose tissue-derived mesenchymal stem cells (ADMSCs) in a self-assembling nano-polypeptide hydrogel environment

2021 ◽  
Vol 10 (1) ◽  
pp. 547-554
Author(s):  
Jianmin Ling ◽  
Ailing Tian ◽  
Xin Yi ◽  
Nianfeng Sun
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Western Blotting ◽  
Three Dimensional ◽  
Culture Conditions ◽  
High Expression ◽  
Expression Levels ◽  
Self Assembling ◽  
Three Dimensional Culture

Abstract To research the paracrine role of adipose tissue-derived mesenchymal stem cells (ADMSCs) in promoting angiogenesis under the three-dimensional culture conditions consisting of a functionalized self-assembling peptide nanofiber hydrogel. ADMSCs were isolated, extracted, and then identified. Three kinds of peptides (RADAI-16, RGD, and KLT) were prepared, and a functionalized self-assembling peptide nanofiber hydrogel was produced by mixing RADAI-16, RGD, and KLT in a volume ratio 2:1:1. AFM was used to observe RADAI-16, RGD, KLT, and the functionalized self-assembling peptide nanofiber hydrogel. Then, ADMSCs were cultured under three-dimensional conditions consisting of the peptide nanofiber hydrogel, and AFM was used to observe cell migration. The ADMSCs in the common culture group (37°C, 5% CO2 cell culture box) and hypoxic culture group (37°C, 10% CO2, and 1% O2 hypoxic culture box) acted as controls. ADMSCs were three-dimensionally cultured in situ for 1 day, and then the concentrations of HGF and VEGF in the supernatant were determined by ELISA. Cells were extracted from the peptide nanofiber hydrogel, and HO-1 expression was detected by western blotting. ADMSCs have high expression levels of CD29, CD90, and CDl05 and low expression levels of CD34 and CD45. In addition, they can differentiate into adipocytes and osteocytes. The diameters of the fibers of RADAI-16, RGD, KLT, and the functionalized self-assembling peptide hydrogel are 17.34 ± 1.82, 15.50 ± 1.41, 13.77 ± 1.18, and 20.26 ± 1.25 nm, respectively. AFM indicated that cells in the functionalized self-assembling peptide nanofiber hydrogel migrated farther than those in RADAI-16. The concentrations of HGF under common, hypoxic, and three-dimensional culture conditions were 47.31 ± 6.75, 247.86 ± 17.59, and 297.25 ± 17.95 pg/mL, respectively, while the concentrations of VEGF were 218.30 ± 3.03, 267.13 ± 4.27, and 289.14 ± 3.11 pg/mL, respectively. Both HGF and VEGF were expressed more in the presence of the functionalized self-assembling peptide nanofiber hydrogel than in its absence (P < 0.05). Using western blotting, ADMSCs cultured under hypoxic and three-dimensional conditions were found to have high expression levels of HO-1. Culturing ADMSCs under three-dimensional conditions consisting of functionalized self-assembling peptide nanofiber hydrogels can promote their paracrine role in angiogenesis, such as HGF and VEGF, and hypoxia is one of the important elements.

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