scholarly journals Retinal cell type expression specificity of HIV-1-derived gene transfer vectors upon subretinal injection in the adult rat: influence of pseudotyping and promoter

2005 ◽  
Vol 7 (10) ◽  
pp. 1367-1374 ◽  
Author(s):  
Alexis-Pierre Bemelmans ◽  
Sébastien Bonnel ◽  
Leïla Houhou ◽  
Noëlle Dufour ◽  
Emeline Nandrot ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Retinal Cell ◽  
Cell Type ◽  
Adult Rat ◽  
Transfer Vectors ◽  
Hiv 1 ◽  
Subretinal Injection

Inhibition of HIV-1 Replication in Alveolar Macrophages by Adenovirus Gene Transfer Vectors

2002 ◽  
Vol 27 (2) ◽  
pp. 214-219 ◽  
Author(s):  
Joshua Rice ◽  
Ruth Connor ◽  
Stefan Worgall ◽  
John P. Moore ◽  
Philip L. Leopold ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Alveolar Macrophages ◽  
Transfer Vectors ◽  
Hiv 1

scholarly journals Novel Tat-Encoding Bicistronic Human Immunodeficiency Virus Type 1-Based Gene Transfer Vectors for High-Level Transgene Expression

2000 ◽  
Vol 74 (14) ◽  
pp. 6659-6668 ◽  
Author(s):  
Narasimhachar Srinivasakumar ◽  
Friedrich Schuening
Keyword(s):  
Human Immunodeficiency Virus ◽  
Gene Transfer ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Transgene Expression ◽  
Packaging System ◽  
Immunodeficiency Virus ◽  
Transfer Vectors ◽  
Hiv 1

ABSTRACT We describe bicistronic single-exon Tat (72-amino-acid Tat [Tat72])- and full-length Tat (Tat86)-encoding gene transfer vectors based on human immunodeficiency virus type 1 (HIV-1). We created versions of these vectors that were rendered Rev independent by using the constitutive transport element (CTE) from Mason-Pfizer monkey virus (MPMV). Tat72-encoding vectors performed better than Tat86-expressing vectors in gene transfer experiments. CTE-containing vectors, produced in a Rev-independent packaging system, had gene transfer efficiencies nearly equivalent to those produced using a combination RNA transport (CTE and Rev-Rev response element)-based packaging system. The Tat72-encoding vectors could be efficiently transduced into a variety of cell types, showed higher levels of transgene expression than vectors with the simian cytomegalovirus immediate-early or the simian virus 40 early promoter, and provide an alternative to HIV-1 vectors with internal promoters.

scholarly journals Evaluation of Tat-Encoding Bicistronic Human Immunodeficiency Virus Type 1 Gene Transfer Vectors in Primary Canine Bone Marrow Mononuclear Cells

2002 ◽  
Vol 76 (14) ◽  
pp. 7334-7342 ◽  
Author(s):  
Narasimhachar Srinivasakumar ◽  
Michail Zaboikin ◽  
Tatiana Zaboikina ◽  
Friedrich Schuening
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Gene Transfer ◽  
Mononuclear Cells ◽  
Bone Marrow Mononuclear Cells ◽  
Immunodeficiency Virus ◽  
Transfer Vectors ◽  
Hiv 1 ◽  
Primary Canine

ABSTRACT Tat-encoding human immunodeficiency virus type 1 (HIV-1) gene transfer vectors were evaluated in primary canine bone marrow mononuclear cells. Tat vectors provided higher levels of gene expression than vectors with internal promoters. The HIV-1 vector was also more efficient than Moloney murine leukemia virus (MoMLV) vectors for transduction of canine bone marrow mononuclear cells in vitro. Transplantation experiments in dogs with transduced autologous marrow cells confirmed the superiority of HIV-1 vectors over MoMLV vectors for gene transfer into canine bone marrow cells. Tat vectors may be useful not only for providing high levels of therapeutic gene expression in hematopoietic cells but also for study of the biological effects of Tat in those tissues in the canine model.

Sign in / Sign up

Export Citation Format

Share Document