Improved non-viral transfection of glial and adult neural stem cell lines and of primary astrocytes by combining agents with complementary modes of action

2004 ◽  
Vol 6 (9) ◽  
pp. 1023-1032 ◽  
Author(s):  
Rogan B. Tinsley ◽  
Melanie J. Vesey ◽  
Shahram Barati ◽  
Robert A. Rush ◽  
Ian A. Ferguson
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lines ◽  
Adult Neural Stem Cell ◽  
Modes Of Action ◽  
Primary Astrocytes ◽  
Stem Cell Lines ◽  
Viral Transfection

Intravascular Delivery of Neural Stem Cell Lines to Target Intracranial and Extracranial Tumors of Neural and Non-Neural Origin

2003 ◽  
Vol 14 (18) ◽  
pp. 1777-1785 ◽  
Author(s):  
Alice B. Brown ◽  
Wendy Yang ◽  
Nils O. Schmidt ◽  
Rona Carroll ◽  
Kim K. Leishear ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lines ◽  
Neural Origin ◽  
Stem Cell Lines

Derivation of Haploid Neural Stem Cell Lines by Selection for a Pax6-GFP Reporter

2018 ◽  
Vol 27 (7) ◽  
pp. 479-487 ◽  
Author(s):  
Qian Gao ◽  
Wenhao Zhang ◽  
Lifang Ma ◽  
Xu Li ◽  
Haisong Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lines ◽  
Gfp Reporter ◽  
Selection For ◽  
Stem Cell Lines

Differential pathotropism of non-immortalized and immortalized human neural stem cell lines in a focal demyelination model

2011 ◽  
Vol 69 (7) ◽  
pp. 1193-1210 ◽  
Author(s):  
Daniela Ferrari ◽  
Cristina Zalfa ◽  
Laura Rota Nodari ◽  
Maurizio Gelati ◽  
Luigi Carlessi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lines ◽  
Human Neural Stem Cell ◽  
Stem Cell Lines ◽  
Focal Demyelination

scholarly journals Systematic Chromosomal Analysis of Cultured Mouse Neural Stem Cell Lines

2011 ◽  
Vol 20 (8) ◽  
pp. 1411-1423 ◽  
Author(s):  
Giuseppe R. Diaferia ◽  
Luciano Conti ◽  
Serena Redaelli ◽  
Monica Cattaneo ◽  
Cesare Mutti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lines ◽  
Chromosomal Analysis ◽  
Stem Cell Lines

Neural Stem Cell Lines for CNS Repair

1999 ◽  
pp. 203-IX ◽  
Author(s):  
Alberto Martínez-Serrano ◽  
Evan Y. Snyder
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lines ◽  
Cns Repair ◽  
Stem Cell Lines

scholarly journals Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies

2018 ◽  
Vol 9 (10) ◽  
Author(s):  
Jessica Rosati ◽  
Daniela Ferrari ◽  
Filomena Altieri ◽  
Silvia Tardivo ◽  
Claudia Ricciolini ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lines ◽  
Cell Therapies ◽  
Human Neural Stem Cell ◽  
Stem Cell Lines

scholarly journals Targeted mass spectrometry for monitoring of neural differentiation

Biology Open ◽  
2021 ◽  
Author(s):  
Rita Sucha ◽  
Martina Kubickova ◽  
Jakub Cervenka ◽  
Marian Hruska-Plochan ◽  
Dasa Bohaciakova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lines ◽  
Neural Differentiation ◽  
Selected Reaction Monitoring ◽  
Neural Cells ◽  
Protein Markers ◽  
Stem Cell Lines

Human multipotent neural stem cells could effectively be used for the treatment of a variety of neurological disorders. However, a defining signature of neural stem cell lines that would be expandable, non-tumorigenic, and differentiate into desirable neuronal/glial phenotype after in vivo grafting is not yet defined. Employing a mass spectrometry approach, based on selected reaction monitoring, we tested a panel of well-described culture conditions, and measured levels of protein markers routinely used to probe neural differentiation, i.e. POU5F1 (OCT4), SOX2, NES, DCX, TUBB3, MAP2, S100B, GFAP, GALC, and OLIG1. Our multiplexed assay enabled us to simultaneously identify the presence of pluripotent, multipotent, and lineage-committed neural cells, thus representing a powerful tool to optimize novel and highly specific propagation and differentiation protocols. The multiplexing capacity of this method permits the addition of other newly identified cell type-specific markers to further increase the specificity and quantitative accuracy in detecting targeted cell populations. Such an expandable assay may gain the advantage over traditional antibody-based assays, and represents a method of choice for quality control of neural stem cell lines intended for clinical use.

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