A synthetic peptide vector system for optimal gene delivery to corneal endothelium

2004 ◽  
Vol 6 (2) ◽  
pp. 185-194 ◽  
Author(s):  
Louise Collins ◽  
John W. Fabre
Keyword(s):  
Gene Delivery ◽  
Synthetic Peptide ◽  
Corneal Endothelium ◽  
Vector System ◽  
Peptide Vector

Efficient gene delivery to primary neuron cultures using a synthetic peptide vector system

2003 ◽  
Vol 125 (1-2) ◽  
pp. 113-120 ◽  
Author(s):  
Louise Collins ◽  
Ayodeji A. Asuni ◽  
Brian H. Anderton ◽  
John W. Fabre
Keyword(s):  
Gene Delivery ◽  
Synthetic Peptide ◽  
Vector System ◽  
Primary Neuron ◽  
Efficient Gene ◽  
Peptide Vector

Efficient Gene Transfer to Rat Fetal Osteoblastic Cells by Synthetic Peptide Vector System

2009 ◽  
Vol 16 (4) ◽  
pp. 368-372 ◽  
Author(s):  
Na Man ◽  
Le Yu ◽  
Fang Zheng ◽  
Yang Li ◽  
Long-ping Wen
Keyword(s):  
Gene Transfer ◽  
Synthetic Peptide ◽  
Vector System ◽  
Osteoblastic Cells ◽  
Efficient Gene ◽  
Peptide Vector

scholarly journals Avian sarcoma leukosis virus receptor-envelope system for simultaneous dissection of multiple neural circuits in mammalian brain

2015 ◽  
Vol 112 (22) ◽  
pp. E2947-E2956 ◽  
Author(s):  
Makoto Matsuyama ◽  
Yohei Ohashi ◽  
Tadashi Tsubota ◽  
Masae Yaguchi ◽  
Shigeki Kato ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Gene Delivery ◽  
Cross Reactivity ◽  
Vector System ◽  
Target Cells ◽  
Specific Gene ◽  
Multiple Target ◽  
Neuronal Systems ◽  
Avian Sarcoma

Pathway-specific gene delivery is requisite for understanding complex neuronal systems in which neurons that project to different target regions are locally intermingled. However, conventional genetic tools cannot achieve simultaneous, independent gene delivery into multiple target cells with high efficiency and low cross-reactivity. In this study, we systematically screened all receptor–envelope pairs resulting from the combination of four avian sarcoma leukosis virus (ASLV) envelopes (EnvA, EnvB, EnvC, and EnvE) and five engineered avian-derived receptors (TVA950, TVBS3, TVC, TVBT, and DR-46TVB) in vitro. Four of the 20 pairs exhibited both high infection rates (TVA–EnvA, 99.6%; TVBS3–EnvB, 97.7%; TVC–EnvC, 98.2%; and DR-46TVB–EnvE, 98.8%) and low cross-reactivity (<2.5%). Next, we tested these four receptor–envelope pairs in vivo in a pathway-specific gene-transfer method. Neurons projecting into a limited somatosensory area were labeled with each receptor by retrograde gene transfer. Three of the four pairs exhibited selective transduction into thalamocortical neurons expressing the paired receptor (>98%), with no observed cross-reaction. Finally, by expressing three receptor types in a single animal, we achieved pathway-specific, differential fluorescent labeling of three thalamic neuronal populations, each projecting into different somatosensory areas. Thus, we identified three orthogonal pairs from the list of ASLV subgroups and established a new vector system that provides a simultaneous, independent, and highly specific genetic tool for transferring genes into multiple target cells in vivo. Our approach is broadly applicable to pathway-specific labeling and functional analysis of diverse neuronal systems.

scholarly journals A novel potent strategy for gene delivery using a single peptide vector as a carrier

1999 ◽  
Vol 27 (17) ◽  
pp. 3510-3517 ◽  
Author(s):  
M. C. Morris ◽  
L. Chaloin ◽  
J. Mery ◽  
F. Heitz ◽  
G. Divita
Keyword(s):  
Gene Delivery ◽  
Peptide Vector ◽  
Single Peptide

LIPOADENOFECTION-MEDIATED GENE DELIVERY TO THE CORNEAL ENDOTHELIUM

1998 ◽  
Vol 65 (1) ◽  
pp. 62-67 ◽  
Author(s):  
Carolina V. Arancibia-C??rcamo ◽  
H. Barbaros Oral ◽  
Dorian O. Haskard ◽  
D. Frank P. Larkin ◽  
Andrew J.T. George
Keyword(s):  
Gene Delivery ◽  
Corneal Endothelium
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