Practical considerations of recombinant adeno-associated virus-mediated gene transfer for treatment of retinal degenerations

2003 ◽  
Vol 5 (7) ◽  
pp. 576-587 ◽  
Author(s):  
Wei-Yong Shen ◽  
Chooi-May Lai ◽  
Yvonne K. Y. Lai ◽  
Dan Zhang ◽  
Tammy Zaknich ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Adeno Associated Virus ◽  
Retinal Degenerations

1509: Adeno-Associated Virus-Mediated Human IL-10 Gene Transfer Suppresses the Development of Experimental Autoimmune Orchitis

2005 ◽  
Vol 173 (4S) ◽  
pp. 409-409
Author(s):  
Masami Watanabe ◽  
Atsushi Nagai ◽  
Norihiro Kusumi ◽  
Yasutomo Nasu ◽  
Hiromi Kumon ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Adeno Associated Virus ◽  
Autoimmune Orchitis ◽  
Experimental Autoimmune

1011: Suppression of Renal Cell Carcinoma Lung Metastasis by Adeno-Associated Virus (AAV) Mediated Gene Transfer of a Soluble Receptor of VEGF

2004 ◽  
Vol 171 (4S) ◽  
pp. 267-267
Author(s):  
Ichiro Yoshimura ◽  
Yasunori Mizuguchi ◽  
Akira Miyajima ◽  
Tomohiko Asano ◽  
Hiroaki Mizukami ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Gene Transfer ◽  
Cell Carcinoma ◽  
Lung Metastasis ◽  
Renal Cell ◽  
Soluble Receptor ◽  
Adeno Associated Virus

Safe and effective gene transfer by adeno-associated virus of neonatal thymus-derived mesenchymal stromal cells

2011 ◽  
Vol 43 (2) ◽  
pp. 108-114 ◽  
Author(s):  
W.I. Lee ◽  
M. Khim ◽  
I.R. Im ◽  
O. Shin ◽  
J.W. Park ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Adeno Associated Virus

scholarly journals Adeno-associated virus-mediated gene transfer to hair cells and support cells of the murine cochlea

2005 ◽  
Vol 11 (6) ◽  
pp. 843-848 ◽  
Author(s):  
Ida M. Stone ◽  
Diana I. Lurie ◽  
Mathew W. Kelley ◽  
David J. Poulsen
Keyword(s):  
Gene Transfer ◽  
Hair Cells ◽  
Adeno Associated Virus

scholarly journals Enhancing the Utility of Adeno-Associated Virus Gene Transfer through Inducible Tissue-Specific Expression

2013 ◽  
Vol 24 (4) ◽  
pp. 270-278 ◽  
Author(s):  
Shu-Jen Chen ◽  
Julie Johnston ◽  
Arbans Sandhu ◽  
Lawrence T. Bish ◽  
Ruben Hovhannisyan ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Specific Expression ◽  
Adeno Associated Virus ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Virus Gene

Cochlear gene transfer mediated by adeno-associated virus: Comparison of two surgical approaches

2015 ◽  
Vol 125 (11) ◽  
pp. 2557-2564 ◽  
Author(s):  
Wade W. Chien ◽  
Devin S. McDougald ◽  
Soumen Roy ◽  
Tracy S. Fitzgerald ◽  
Lisa L. Cunningham
Keyword(s):  
Gene Transfer ◽  
Surgical Approaches ◽  
Adeno Associated Virus

scholarly journals Virus-Mediated Transduction of Murine Retina with Adeno-Associated Virus: Effects of Viral Capsid and Genome Size

2002 ◽  
Vol 76 (15) ◽  
pp. 7651-7660 ◽  
Author(s):  
Grace S. Yang ◽  
Michael Schmidt ◽  
Ziying Yan ◽  
Jonathan D. Lindbloom ◽  
Thomas C. Harding ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Photoreceptor Cells ◽  
Cell Types ◽  
Full Length ◽  
Specific Cell ◽  
Adeno Associated Virus ◽  
Gene Therapy Vectors ◽  
Retinal Degenerative Diseases ◽  
Or Gene ◽  
Half Length

ABSTRACT Gene therapy vectors based on adeno-associated viruses (AAVs) show promise for the treatment of retinal degenerative diseases. In prior work, subretinal injections of AAV2, AAV5, and AAV2 pseudotyped with AAV5 capsids (AAV2/5) showed variable retinal pigmented epithelium (RPE) and photoreceptor cell transduction, while AAV2/1 predominantly transduced the RPE. To more thoroughly compare the efficiencies of gene transfer of AAV2, AAV3, AAV5, and AAV6, we quantified, using stereological methods, the kinetics and efficiency of AAV transduction to mouse photoreceptor cells. We observed persistent photoreceptor and RPE transduction by AAV5 and AAV2 up to 31 weeks and found that AAV5 transduced a greater volume than AAV2. AAV5 containing full-length or half-length genomes and AAV2/5 transduced comparable numbers of photoreceptor cells with similar rates of onset of expression. Compared to AAV2, AAV5 transduced significantly greater numbers of photoreceptor cells at 5 and 15 weeks after surgery (greater than 1,000 times and up to 400 times more, respectively). Also, there were 30 times more genome copies in eyes injected with AAV2/5 than in eyes injected with AAV2. Comparing AAVs with half-length genomes, AAV5 transduced only four times more photoreceptor cells than AAV2 at 5 weeks and nearly equivalent numbers at 15 weeks. The enhancement of transduction was seen at the DNA level, with 50 times more viral genome copies in retinas injected with AAV having short genomes than in retinas injected with AAV containing full-length ones. Subretinal injection of AAV2/6 showed only RPE transduction at 5 and 15 weeks, while AAV2/3 did not transduce retinal cells. We conclude that varying genome length and AAV capsids may allow for improved expression and/or gene transfer to specific cell types in the retina.

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