Sustained transgene expression in cartilage defectsin vivo after transplantation of articular chondrocytes modified by lipid-mediated gene transfer in a gel suspension delivery system

2003 ◽  
Vol 5 (6) ◽  
pp. 502-509 ◽  
Author(s):  
Henning Madry ◽  
Magali Cucchiarini ◽  
Ute Stein ◽  
Klaus Remberger ◽  
Michael D. Menger ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Delivery System ◽  
Transgene Expression ◽  
Articular Chondrocytes

Comparison of long-term transgene expression after non-viral and adenoviral gene transfer into primary articular chondrocytes

2001 ◽  
Vol 116 (1) ◽  
pp. 69-77 ◽  
Author(s):  
Robert Dinser ◽  
Florian Kreppel ◽  
Frank Zaucke ◽  
Christoph Blank ◽  
Mats Paulsson ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Transgene Expression ◽  
Articular Chondrocytes ◽  
Adenoviral Gene Transfer

Polyplex: A Promising Gene Delivery System

2019 ◽  
Vol 12 (6) ◽  
pp. 4681-4686
Author(s):  
Rohan Aggarwal ◽  
Monika Targhotra ◽  
Bhumika Kumar ◽  
P.K Sahoo ◽  
Meenakshi K Chauhan
Keyword(s):  
Gene Transfer ◽  
Gene Delivery ◽  
Delivery System ◽  
Viral Vectors ◽  
Gene Delivery System ◽  
Cationic Polymers ◽  
Therapeutic Application ◽  
Promising Alternative ◽  
Structure And Stability ◽  
The Past

In the past few years gene delivery system has gained a huge attention owing to its proved efficacy in several diseases especially in those caused by genetic and/oroncological malfunctioning. The effective gene delivery mainly depends on the carrier molecules that can ensure the safe and specific delivery of the nucleic acidmolecules. Viral vectors have been used for a longer period as the gene transfer vehicle. However, these viral vectors have potential immunological disadvantages that made them less preferred. Recently, non-viral vectors such as polyplexes have emerged as a promising alternative for viral vectors. Polyplexes are formed by conjugating a polymer with DNA and in maximum cases the cationic polymers are preferred over others. The structure and stability of the polyplexes depends on various factors. The ability of the polymer to condense the DNA mainly dictates the efficiency of the polyplex mediated transfection. In this review we are going to provide a framework for the synthesis and design of the polyplexes along with the structure and stability of the complexes pertaining to mechanism of action, characterization and therapeutic application, including polyethyleneimine mediated cytotoxicity as well as newer strategies for the generation of better polyplexes.

scholarly journals Retrograde Transgene Expression via Neuron-Specific Lentiviral Vector Depends on Both Species and Input Projections

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1387
Author(s):  
Yukiko Otsuka ◽  
Hitomi Tsuge ◽  
Shiori Uezono ◽  
Soshi Tanabe ◽  
Maki Fujiwara ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Immune Responses ◽  
Envelope Glycoprotein ◽  
Transgene Expression ◽  
Histological Analysis ◽  
Lentiviral Vector ◽  
Cortical Input ◽  
Input System ◽  
Vesicular Stomatitis ◽  
Retrograde Gene Transfer

For achieving retrograde gene transfer, we have so far developed two types of lentiviral vectors pseudotyped with fusion envelope glycoprotein, termed HiRet vector and NeuRet vector, consisting of distinct combinations of rabies virus and vesicular stomatitis virus glycoproteins. In the present study, we compared the patterns of retrograde transgene expression for the HiRet vs. NeuRet vectors by testing the cortical input system. These vectors were injected into the motor cortex in rats, marmosets, and macaques, and the distributions of retrograde labels were investigated in the cortex and thalamus. Our histological analysis revealed that the NeuRet vector generally exhibits a higher efficiency of retrograde gene transfer than the HiRet vector, though its capacity of retrograde transgene expression in the macaque brain is unexpectedly low, especially in terms of the intracortical connections, as compared to the rat and marmoset brains. It was also demonstrated that the NeuRet but not the HiRet vector displays sufficiently high neuron specificity and causes no marked inflammatory/immune responses at the vector injection sites in the primate (marmoset and macaque) brains. The present results indicate that the retrograde transgene efficiency of the NeuRet vector varies depending not only on the species but also on the input projections.

CFTR Transgene Expression in Primary ΔF508 Epithelial Cell Cultures From Human Nasal Polyps Following Gene Transfer With Cationic Phosphonolipids

2004 ◽  
Vol 26 (3) ◽  
pp. 193-206 ◽  
Author(s):  
Tristan Montier ◽  
Pascal Delépine ◽  
Rémi Marianowski ◽  
Karine Le Ny ◽  
Morgane Le Bris ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Epithelial Cell ◽  
Cell Cultures ◽  
Transgene Expression ◽  
Nasal Polyps ◽  
Epithelial Cell Cultures

Effect of collagen based vector delivery system on the efficiency of adenoviral mediated gene transfer and wound healing

2000 ◽  
Vol 191 (4) ◽  
pp. S45-S46
Author(s):  
Colette R Pameijer ◽  
Sruthi Tallapragada ◽  
Robert Levy ◽  
Timothy M Crombleholme
Keyword(s):  
Wound Healing ◽  
Gene Transfer ◽  
Delivery System ◽  
Vector Delivery

Gene transfer to schwann cells after peripheral nerve injury: A delivery system for therapeutic agents

1998 ◽  
Vol 43 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Jesper Sørensen ◽  
Georg Haase ◽  
Christian Krarup ◽  
Helene Gilgenkrantz ◽  
Axel Kahn ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Delivery System ◽  
Peripheral Nerve Injury ◽  
Therapeutic Agents
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