LINC00852 is associated with poor prognosis in non‐small cell lung cancer patients and its inhibition suppresses cancer cell proliferation and chemoresistance via the hsa‐miR‐145‐5p/KLF4 axis

2021 ◽  
Author(s):  
Zineng Tuo ◽  
Li Liang ◽  
Rongmei Zhou
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Small Cell Lung Cancer ◽  
Cancer Patients ◽  
Cancer Cell ◽  
Poor Prognosis ◽  
Small Cell ◽  
Cancer Cell Proliferation ◽  
Small Cell Lung ◽  
Lung Cancer Patients

scholarly journals A novel long non-coding RNA LSAMP-1 is down-regulated in non-small cell lung cancer and predicts a poor prognosis

2020 ◽  
Author(s):  
wei gong ◽  
Fuman Qiu ◽  
Yinyan Li ◽  
Lei Yang ◽  
Yuanyuan Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Tumor Suppressor ◽  
Small Cell Lung Cancer ◽  
Cancer Cell ◽  
Poor Prognosis ◽  
Small Cell ◽  
Cancer Cell Proliferation ◽  
Lung Cancer Cell ◽  
Small Cell Lung

Abstract Background There is very little known about how long non-coding RNAs (lncRNAs) are associated with membrane proteins in lung cancer. The limbic system-associated membrane protein ( LSAMP ) has been reported to play a tumor suppressor role in a variety of cancers.Methods We aimed to explore the lncRNA associated with LSAMP and explore its effects on lung cancer.Results We found that Lnc-LSAMP-1 was significantly down-regulated in 170 cases of lung tumor tissues when compared to the adjacent non-cancerous tissues (p<0.001). Our results indicated that low expression of Lnc-LSAMP-1 was significantly correlated with stage (TNM)(p=0.006), N status (p=0.009) and poor prognosis (p=0.004). Further investigation showed that overexpression of Lnc-LSAMP-1 significantly inhibited lung cancer cell proliferation, viability, invasion and migration ability, arrested cell cycle and facilitated apoptosis. Meanwhile, the expression of Lnc-LSAMP-1 is highly correlated with the expression of nearby tumor suppressor gene LSAMP in our samples (r=0.7074, p<0.001) and TCGA database (r=0.78, p<0.001). It was found that overexpression of Lnc-LSAMP-1 can slow down the degradation rate of LSAMP gene by mRNA protection experiments. By knocking down of LSAMP gene, it was found that overexpressed Lnc-LSAMP-1 cells showed a high proliferation rate. Chemotherapy sensitization experiments showed that overexpression of Lnc-LSAMP-1 enhanced TKI inhibition of lung cancer cell proliferation, which is probably related to affecting the prognosis of patients.Conclusions Consequently, the above data suggested that Lnc-LSAMP-1 functions as a tumor suppressor and provides a new potentially therapeutic and prognostic target for non-small cell lung cancer.

scholarly journals Long non-coding RNA FGD5-AS1 promotes non-small cell lung cancer cell proliferation through sponging hsa-miR-107 to up-regulate FGFRL1

2020 ◽  
Vol 40 (1) ◽  
Author(s):  
Yafeng Fan ◽  
Hongxia Li ◽  
Zhongping Yu ◽  
Wen Dong ◽  
Xiaoyan Cui ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Small Cell Lung Cancer ◽  
Cancer Cell ◽  
Small Cell ◽  
Cancer Cell Proliferation ◽  
Lung Cancer Cell ◽  
Small Cell Lung ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Abstract Long non-coding RNA (lncRNA) FYVE, RhoGEF and PH domain containing 5 antisense RNA 1 (FGD5-AS1) has been reported as an oncogene in colorectal cancer, promoting its tumorgenesis. The present paper focused on searching the potential function of FGD5-AS1 in non-small cell lung carcinoma (NSCLC). There are connections between the expression of lncRNA FGD5-AS1 and human NSCLC tumor growth and progression. Also, the relationships between FGD5-AS1, hsa-miR-107 and mRNA fibroblast growth factor receptor like 1 (FGFRL1) are going to test their interaction in NSCLC cell lines, which may cause a series of biological behaviors of NSCLC cells. qRT-PCR analysis was conducted to test the expression of RNAs in different situation. CCK-8 experiment and clone formation assay were performed to assess proliferation of NSCLC cells. Also, connection between FGD5-AS1 and hsa-miR-107 were investigated by luciferase reporter assay and RNA pull-down assay. Rescue experiments were performed to verify the modulating relationship between FGD5-AS1, hsa-miR-107 and FGFRL1. High-level expression of FGD5-AS1 was found in NSCLC. FGD5-AS1 may promote the proliferation of NSCLC cells. Also, the combination between hsa-miR-107, FGD5-AS1 and NSCLC have been proved, which means they can play an interaction function in NSCLC cells. Thence, we concluded that lncRNA FGD5-AS1 promotes non-small cell lung cancer cell proliferation through sponging hsa-miR-107 to up-regulate FGFRL1.

scholarly journals Glyceraldehyde-3-phosphate dehydrogenase gene over expression correlates with poor prognosis in non small cell lung cancer patients

2013 ◽  
Vol 12 (1) ◽  
pp. 97 ◽  
Author(s):  
Roberto Puzone ◽  
Graziana Savarino ◽  
Sandra Salvi ◽  
Maria Dal Bello ◽  
Giulia Barletta ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Patients ◽  
Cell Lung Cancer ◽  
Poor Prognosis ◽  
Small Cell ◽  
Small Cell Lung ◽  
Lung Cancer Patients ◽  
Over Expression ◽  
Dehydrogenase Gene
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