Development and characterization of a minimal inducible packaging cell line for simian immunodeficiency virus-based lentiviral vectors

2002 ◽  
Vol 4 (4) ◽  
pp. 347-355 ◽  
Author(s):  
Seraphin Kuate ◽  
Ralf Wagner ◽  
Klaus Überla
Keyword(s):  
Cell Line ◽  
Simian Immunodeficiency Virus ◽  
Lentiviral Vectors ◽  
Packaging Cell Line ◽  
Packaging Cell ◽  
Immunodeficiency Virus

Highly efficient gene transfer into baboon marrow repopulating cells using GALV-pseudotype oncoretroviral vectors produced by human packaging cells

Blood ◽  
2002 ◽  
Vol 100 (12) ◽  
pp. 3960-3967 ◽  
Author(s):  
Peter A. Horn ◽  
Max S. Topp ◽  
Julia C. Morris ◽  
Stanley R. Riddell ◽  
Hans-Peter Kiem
Keyword(s):  
Stem Cells ◽  
Gene Transfer ◽  
Cell Line ◽  
Flow Cytometric Analysis ◽  
Lentiviral Vectors ◽  
Transduction Efficiency ◽  
Packaging Cell Line ◽  
Hematopoietic Stem ◽  
Packaging Cell ◽  
Cd34 Cells

Vector-containing medium harvested from murine packaging cell lines has been shown to contain factors that can negatively influence the transduction and maintenance of hematopoietic stem cells. Thus, we generated a human packaging cell line with a gibbon ape leukemia virus pseudotype (Phoenix-GALV), and we evaluated vectors produced by Phoenix-GALV for their ability to transduce hematopoietic progenitor/stem cells. In 3 baboons, we used a competitive repopulation assay to directly compare GALV-pseudotype retrovirus vectors produced by either Phoenix-GALV or by the NIH 3T3–derived packaging cell line, PG13. In 3 additional baboons we compared Phoenix-GALV–derived vectors to more recently developed lentiviral vectors. Gene transfer efficiency into hematopoietic repopulating cells was assessed by evaluating the number of genetically modified peripheral blood and marrow cells using flow cytometry and real-time polymerase chain reaction. Transduction efficiency of hematopoietic repopulating cells was significantly higher using the Phoenix-GALV–derived vector as compared with the PG13-derived vectors or lentiviral vectors, with stable transduction levels up to 25%. We followed 2 animals for more than one year. Flow cytometric analysis of hematopoietic subpopulations in these animals revealed transgene expression in CD13+ granulocytes, CD20+ B lymphocytes, CD3+ T lymphocytes, CD61+ platelets, as well as red blood cells, indicating multilineage engraftment of cells transduced by Phoenix-GALV–pseudotype vectors. In addition, transduction of human CD34+ cells was significantly more efficient than transduction of baboon CD34+ cells, suggesting that Phoenix-GALV–derived oncoretroviral vectors may be even more efficient in human stem cell gene therapy applications.

scholarly journals RD2-MolPack-Chim3,a Packaging Cell Line for Stable Production of Lentiviral Vectors for Anti-HIV Gene Therapy

2013 ◽  
Vol 24 (4) ◽  
pp. 228-240 ◽  
Author(s):  
Anna Stornaiuolo ◽  
Bianca Maria Piovani ◽  
Sergio Bossi ◽  
Eleonora Zucchelli ◽  
Stefano Corna ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cell Line ◽  
Lentiviral Vectors ◽  
Packaging Cell Line ◽  
Packaging Cell ◽  
Stable Production ◽  
Anti Hiv

A New-Generation Stable Inducible Packaging Cell Line for Lentiviral Vectors

2001 ◽  
Vol 12 (8) ◽  
pp. 981-997 ◽  
Author(s):  
Deborah Farson ◽  
Rochelle Witt ◽  
Ryan McGuinness ◽  
Tom Dull ◽  
Michael Kelly ◽  
...  
Keyword(s):  
Cell Line ◽  
Lentiviral Vectors ◽  
Packaging Cell Line ◽  
Packaging Cell ◽  
New Generation

Construction and characterization of a packaging cell line for MMTV-based conditional retroviral vectors

1989 ◽  
Vol 159 (3) ◽  
pp. 1191-1198 ◽  
Author(s):  
Brian Salmons ◽  
Sylviane Moritz-Legrand ◽  
Iqbal Garcha ◽  
Walter H. Günzburg
Keyword(s):  
Cell Line ◽  
Retroviral Vectors ◽  
Packaging Cell Line ◽  
Packaging Cell
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