Osteoinduction by repeat plasmid injection of human bone morphogenetic protein-2

2010 ◽  
Vol 12 (12) ◽  
pp. 937-944 ◽  
Author(s):  
Kenji Osawa ◽  
Yasunori Okubo ◽  
Kazumasa Nakao ◽  
Noriaki Koyama ◽  
Kazuhisa Bessho
Keyword(s):  
Bone Morphogenetic Protein ◽  
Human Bone ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Plasmid Injection ◽  
Human Bone Morphogenetic Protein

Tooth Eruption into the Newly Generated Bone Induced by Recombinant Human Bone Morphogenetic Protein-2

2002 ◽  
Vol 39 (4) ◽  
pp. 449-456 ◽  
Author(s):  
Atsushi Kitamura ◽  
Nobuyoshi Motohashi ◽  
Tatsuo Kawamoto ◽  
Yoshiyuki Baba ◽  
Shoichi Suzuki ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Alveolar Bone ◽  
Treated Group ◽  
Tooth Eruption ◽  
Human Bone ◽  
Bone Morphogenetic Protein 2 ◽  
Mineral Density ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Recombinant Human Bone

Objective The purpose of this study was to observe tooth eruption pattern into the newly generated bone induced by recombinant human bone morphogenetic protein-2 (rhBMP-2). Method Three 12-week-old beagle dogs were used. Following wound healing of the extracted maxillary third deciduous incisor at the age of 13 weeks, a resection of maxillary alveolar bone (8 mm by 8 mm by 8 mm) was carried out, accompanied by extraction of the maxillary second incisor tooth germ. The rhBMP-2 (5 μg/100 μL or 10 μg/100 μL) was implanted in the resected area. A group that was not implanted after resection and a nonresected group were used as controls. Serial changes of the eruption pattern of the maxillary third incisor in the implanted area were observed radiographically. Oxytetracycline and calcein were employed as bone markers. Nine weeks after the implantation, the animals were sacrificed. The samples were observed histologically and analyzed using peripheral quantitative computerized tomography to acquire the bone mineral density (BMD). Results In the rhBMP-2 (5 μg/100 μL)-treated group, histological findings and the BMD of the newly generated bone were almost same as in the non-resected group. The maxillary third incisor erupted through the implanted area. However, in the rhBMP-2 (10 μg/100 μL)-treated group, more osteoinductive activity and significantly higher BMD were observed in the newly generated bone. The maxillary third incisor erupted mesially so as to avoid this implanted area. Conclusion Results showed that bone remodeling and tooth eruption through the newly generated bone appear to be dose dependent. Accordingly, proper dosage of rhBMP-2 should be determined for successful clinical application.

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