Protective immunity against porcine circovirus 2 in mice induced by a gene-based combination vaccination

2009 ◽  
Vol 11 (4) ◽  
pp. 288-301 ◽  
Author(s):  
Kandan Aravindaram ◽  
Tsun-Yung Kuo ◽  
Chun-Wen Lan ◽  
Hsiu-Hui Yu ◽  
Pei-Hsueh Wang ◽  
...  
Keyword(s):  
Protective Immunity ◽  
Porcine Circovirus ◽  
Porcine Circovirus 2

scholarly journals Protective immunity against porcine circovirus 2 by vaccination with ORF2-based DNA and subunit vaccines in mice

2008 ◽  
Vol 89 (8) ◽  
pp. 1857-1865 ◽  
Author(s):  
Hui-Gang Shen ◽  
Ji-Yong Zhou ◽  
Zhen-Yu Huang ◽  
Jun-Qing Guo ◽  
Gang Xing ◽  
...  
Keyword(s):  
T Cells ◽  
Protective Immunity ◽  
Flow Cytometric Analysis ◽  
Antibody Responses ◽  
Pcv2 Infection ◽  
Porcine Circovirus ◽  
Comparable Efficacy ◽  
Histopathological Analysis ◽  
Subunit Vaccines ◽  
Porcine Circovirus 2

The protective immune response against porcine circovirus 2 (PCV2) infection in mice was characterized using flow cytometric analysis (FCM), assays of antibody (of different IgG isotypes) and viraemia, and histopathological examination. An open reading frame 2 plasmid (pORF2) and the capsid protein (Cap) of PCV2 were used as DNA and subunit vaccines, respectively. In FCM analysis, although pORF2 and Cap alone showed comparable efficacy in eliciting lymphoproliferative responses and Cap-specific CD4+ T cells, pORF2 was superior to the Cap protein in triggering CD8+ T cells. A virus neutralization assay showed that pORF2 evoked stronger recall virus-neutralizing (VN) antibody responses than the Cap protein on PCV2 challenge. Correspondingly, VN antibody kinetics coincided with those of Cap-specific IgG2a, but not with the kinetics of IgG and IgG1. Following virus challenge, real-time PCR and histopathological analysis confirmed that only low viral DNA loads and mild microscopic lesions appeared in pORF2-immunized mice. These findings indicate that CD8+ T cells and VN antibody responses correlating mainly with Cap-specific IgG2a play crucial roles in protecting against PCV2 infection, and that the protective immunity induced by the pORF2 plasmid is superior to that induced by the PCV2 Cap protein.

Protective immunity conferred by porcine circovirus 2 ORF2-based DNA vaccine in mice

2014 ◽  
Vol 58 (7) ◽  
pp. 398-408 ◽  
Author(s):  
Seydou Sylla ◽  
Yan-Long Cong ◽  
Yi-Xue Sun ◽  
Gui-Lian Yang ◽  
Xue-Mei Ding ◽  
...  
Keyword(s):  
Dna Vaccine ◽  
Protective Immunity ◽  
Porcine Circovirus ◽  
Porcine Circovirus 2

scholarly journals Exploratory field study on the effects of porcine circovirus 2 (PCV-2) sow vaccination at different physiological stages mimicking blanket vaccination

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Patricia Pleguezuelos ◽  
Marina Sibila ◽  
Raúl Cuadrado ◽  
Rosa López-Jiménez ◽  
Diego Pérez ◽  
...  
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Negative Control Group ◽  
Negative Control ◽  
Late Gestation ◽  
Antibody Detection ◽  
Porcine Circovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Physiological Status ◽  
Porcine Circovirus 2

Abstract Background The objective of the present study was to explore the benefits of Porcine circovirus 2 (PCV-2) blanket vaccination in a sow herd on productive parameters, PCV-2 infection and immune status in sows and their progeny. For this purpose, 288 sows were distributed among four balanced experimental groups. One group remained as negative control group and the other three received 1 mL of PCV-2 Ingelvac Circoflex® intramuscularly at different productive cycle moments: before mating, mid gestation (42–49 days post-insemination) or late gestation (86–93 days post-insemination); phosphate buffered saline (PBS) was used as negative control item. Reproductive parameters from sows during gestation and body weight of their progeny from birth to weaning were recorded. Additionally, blood was collected from sows at each vaccination time and piglets at 3 weeks of age. Moreover, up to 4 placental umbilical cords (PUC) per sow were taken at peri-partum. Sera from sows and piglets were analysed for PCV-2 antibody detection using an enzyme-linked immunosorbent assay (ELISA). Sera from sows and PUC were tested to quantify viraemia using a real time quantitative polymerase chain reaction (qPCR) assay. Results Globally, results indicated that vaccinated sows showed heavier piglets at birth and at weaning, less cross-fostered piglets, lower viral load at farrowing as well as in PUC, and higher antibody levels at farrowing, compared to non-vaccinated ones. When all groups were compared among them, sows vaccinated at mid or late gestation had heavier piglets at birth than non-vaccinated sows, and lower proportion of PCV-2 positive PUC. Also, cross-fostering was less frequently practiced in sows vaccinated at pre-mating or mid gestation compared to non-vaccinated ones. Conclusions In conclusion, the present study points out that PCV-2 sow vaccination at different time points of their physiological status (mimicking blanket vaccination) offers benefits at production and serological and virological levels.

Correlates of Protection Following Vaccination with Inactivated Porcine Circovirus 2 Vaccines

2015 ◽  
Vol 28 (10) ◽  
pp. 600-608 ◽  
Author(s):  
Cinzia Zanotti ◽  
Nicola Martinelli ◽  
Davide Lelli ◽  
Massimo Amadori
Keyword(s):  
Porcine Circovirus ◽  
Correlates Of Protection ◽  
Porcine Circovirus 2

Cell tropism and entry of porcine circovirus 2

2012 ◽  
Vol 164 (1-2) ◽  
pp. 43-45 ◽  
Author(s):  
H.J. Nauwynck ◽  
R. Sanchez ◽  
P. Meerts ◽  
D.J. Lefebvre ◽  
D. Saha ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Cell Tropism ◽  
Porcine Circovirus 2

scholarly journals Circovírus suíno 2b: isolamento e taxa de mutação em linhagem de célula de macrófagos (J744)

2017 ◽  
Vol 84 (0) ◽  
Author(s):  
Alessandra Marnie Martins Gomes de Castro ◽  
Josete Garcia Bersano ◽  
Sheila Oliveira Silva Souza ◽  
Renato Akio Ogata ◽  
Julia Mitico Nara ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Porcine Circovirus 2

RESUMO: Porcine circovirus 2 (PCV2) está associado a vários sinais clínicos que são designados coletivamente como Circovirose e tem grande impacto na suinocultura. O isolamento viral é classicamente realizado em células da linhagem PK-15, contudo outras células têm sido testadas. Apesar dos avanços nos estudos com PCV2, o isolamento ainda é um desafio. Diante da dificuldade de manutenção dessas linhagens celulares comumente utilizadas associadas à necessidade do uso de substâncias tóxicas para o isolamento de PCV2, os objetivos do presente trabalho foram descrever o primeiro isolamento de Porcine circovirus 2b em linhagens de células de macrófago (J744) e verificar a taxa de mutação nesse sistema. Uma amostra de pulmão foi submetida ao sequenciamento e agrupada ao genótipo PCV2b. Essa amostra foi utilizada para inocular uma garrafa de J744 (com 30% de confluência em meio RPMI com 10% de soro fetal bovino) e submetida a cinco passagens, as quais foram acompanhadas por reação em cadeia da polimerase quantitativa (PCRq). As cargas virais inicial e final foram de 2,90 × 103 e de 4,45 × 108 cópias de DNA/µL para PCV2b, respectivamente. O sequenciamento confirmou o isolamento e descartou o coisolamento de mais de um genótipo. Após cinco passagens, o isolado apresentou identidade de 99,7%, com descrição de cinco mutações pontuais, uma sinônima e quatro não sinônimas, observadas nas regiões do gene cap e rep. Os resultados obtidos demonstram que as células J744 apresentam a susceptibilidade, e a instabilidade do vírus em J744 será importante para a compreensão do vírus.

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