Local matrix metalloproteinase 2 gene knockdown in balloon-injured rabbit carotid arteries using nonviral-small interfering RNA transfection

2009 ◽  
Vol 11 (1) ◽  
pp. 92-99 ◽  
Author(s):  
Hanna Hlawaty ◽  
Aurélie San Juan ◽  
Marie-Paule Jacob ◽  
Roger Vranckx ◽  
Didier Letourneur ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Small Interfering Rna ◽  
Carotid Arteries ◽  
Gene Knockdown ◽  
Matrix Metalloproteinase 2 ◽  
Rna Transfection ◽  
Interfering Rna

scholarly journals Adenovirus-mediated small interfering RNA against matrix metalloproteinase-2 suppresses tumor growth and lung metastasis in mice

2006 ◽  
Vol 5 (9) ◽  
pp. 2289-2299 ◽  
Author(s):  
Chandramu Chetty ◽  
Praveen Bhoopathi ◽  
Pushpa Joseph ◽  
Subramanyam Chittivelu ◽  
Jasti S. Rao ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Tumor Growth ◽  
Lung Metastasis ◽  
Small Interfering Rna ◽  
Matrix Metalloproteinase 2 ◽  
Interfering Rna

scholarly journals Optimization Procedure for Small Interfering RNA Transfection in a 384-Well Format

2007 ◽  
Vol 12 (4) ◽  
pp. 546-559 ◽  
Author(s):  
Jason Borawski ◽  
Alicia Lindeman ◽  
Frank Buxton ◽  
Mark Labow ◽  
L. Alex Gaither
Keyword(s):  
High Throughput Screening ◽  
Mammalian Cells ◽  
Small Interfering Rna ◽  
Dna Analysis ◽  
Lentiviral Vector ◽  
Mrna Levels ◽  
Sirna Transfection ◽  
Rna Transfection ◽  
Well Assay ◽  
Interfering Rna

High-throughput screening of RNAi libraries has become an essential part of functional analysis in academic and industrial settings. The transition of a cell-based RNAi assay into a 384-well format requires several optimization steps to ensure the phenotype being screened is appropriately measured and that the signal-to-background ratio is above a certain quantifiable threshold. Methods currently used to assess small interfering RNA (siRNA) efficacy after transfection, including quantitative PCR or branch DNA analysis, face several technical limitations preventing the accurate measurement of mRNA levels in a 384-well format. To overcome these difficulties, the authors developed an approach using a viral-based transfection system that measures siRNA efficacy in a standardized 384-well assay. This method allows measurement of siRNA activity in a phenotypically neutral manner by quantifying the knockdown of an exogenous luciferase gene delivered by a lentiviral vector. In this assay, the efficacy of a luciferase siRNA is compared to a negative control siRNA across many distinct assay parameters including cell type, cell number, lipid type, lipid volume, time of the assay, and concentration of siRNA. Once the siRNA transfection is optimized as a 384-well luciferase knockdown, the biologically relevant phenotypic analysis can proceed using the best siRNA transfection conditions. This approach provides a key technology for 384-well assay development when direct measurement of mRNA knockdown is not possible. It also allows for direct comparison of siRNA activity across cell lines from almost any mammalian species. Defining optimal conditions for siRNA delivery into mammalian cells will greatly increase the speed and quality of large-scale siRNA screening campaigns. ( Journal of Biomolecular Screening 2007:546-559)

Kallikrein gene ‘knock-down’ by small interfering RNA transfection induces a profibrotic phenotype in rat mesangial cells

2008 ◽  
Vol 26 (1) ◽  
pp. 93-101 ◽  
Author(s):  
Izabella ZA Pawluczyk ◽  
Eddie KC Tan ◽  
David Lodwick ◽  
Kevin PG Harris
Keyword(s):  
Small Interfering Rna ◽  
Mesangial Cells ◽  
Knock Down ◽  
Rna Transfection ◽  
Interfering Rna

Biomimetic gold nanocomplexes for gene knockdown: Will gold deliver dividends for small interfering RNA nanomedicines?

Nano Research ◽  
2015 ◽  
Vol 8 (10) ◽  
pp. 3111-3140 ◽  
Author(s):  
Jianfeng Guo ◽  
Kamil Rahme ◽  
Kathleen A. Fitzgerald ◽  
Justin D. Holmes ◽  
Caitriona M. O’Driscoll
Keyword(s):  
Small Interfering Rna ◽  
Gene Knockdown ◽  
Interfering Rna

GW24-e2948 Small interfering RNA transfection in cultured cardiac fibroblasts

Heart ◽  
2013 ◽  
Vol 99 (Suppl 3) ◽  
pp. A10.2-A10
Author(s):  
Zhu Jia-bao ◽  
Wu Yu-zhou ◽  
Ma Qian-li ◽  
Li Shu-qin
Keyword(s):  
Small Interfering Rna ◽  
Cardiac Fibroblasts ◽  
Rna Transfection ◽  
Interfering Rna

Dendrimers as Vectors for Small Interfering RNA Transfection in the Nervous System

2013 ◽  
pp. 134-147
Author(s):  
F. C. Pérez-Martínez ◽  
A.V. Ocaña ◽  
G.M. Pavan ◽  
A. Danani ◽  
V. Ceña
Keyword(s):  
Nervous System ◽  
Small Interfering Rna ◽  
Rna Transfection ◽  
Interfering Rna
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