scholarly journals S413-PV cell penetrating peptide and cationic liposomes act synergistically to mediate intracellular delivery of plasmid DNA

2008 ◽  
Vol 10 (11) ◽  
pp. 1210-1222 ◽  
Author(s):  
Sara Trabulo ◽  
Miguel Mano ◽  
Henrique Faneca ◽  
Ana Luísa Cardoso ◽  
Sónia Duarte ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Intracellular Delivery ◽  
Cationic Liposomes ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Pv Cell

Porous Silicon–Cell Penetrating Peptide Hybrid Nanocarrier for Intracellular Delivery of Oligonucleotides

2013 ◽  
Vol 11 (2) ◽  
pp. 382-390 ◽  
Author(s):  
Jussi Rytkönen ◽  
Piret Arukuusk ◽  
Wujun Xu ◽  
Kaido Kurrikoff ◽  
Ülo Langel ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Intracellular Delivery ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Silicon Cell

scholarly journals Codelivery of a cytotoxin and photosensitiser via a liposomal nanocarrier: a novel strategy for light-triggered cytosolic release

Nanoscale ◽  
2018 ◽  
Vol 10 (43) ◽  
pp. 20366-20376 ◽  
Author(s):  
Elnaz Yaghini ◽  
Ruggero Dondi ◽  
Karen J. Edler ◽  
Marilena Loizidou ◽  
Alexander J. MacRobert ◽  
...  
Keyword(s):  
Intracellular Delivery ◽  
Cell Penetrating Peptide ◽  
Protein Toxin ◽  
Cell Penetrating ◽  
A Cell ◽  
Novel Strategy

Light-triggered intracellular delivery of a protein toxin was achieved by codelivery via a liposomal nanocarrier, targeted with a cell-penetrating peptide (CPP)–photosensitiser conjugate.

scholarly journals A simple and efficient transfection protocol for Cryptosporidium parvum using Polyethylenimine (PEI) and Octaarginine

Parasitology ◽  
2020 ◽  
Vol 147 (10) ◽  
pp. 1065-1070
Author(s):  
Tran Nguyen-Ho-Bao ◽  
Maxi Berberich ◽  
Wanpeng Zheng ◽  
Dieter Seebach ◽  
Arwid Daugschies ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cryptosporidium Parvum ◽  
Plasmid Dna ◽  
Cationic Polymer ◽  
Cell Penetrating Peptide ◽  
Laboratory Equipment ◽  
Short Cell ◽  
Transfection Protocol ◽  
Simple Protocol ◽  
Cell Penetrating

AbstractThe transfection of Cryptosporidium represents a major challenge, and current protocols are based on electroporation of freshly excysted sporozoites using a rather large amount of plasmid DNA which typically has a very poor yield. In this study, we report a fast and simple protocol for transfection of Cryptosporidium parvum that takes advantage of the DNA condensing power of the poly cationic polymer polyethylenimine (PEI) and the gene delivery property of the short cell-penetrating peptide octaarginine. Our novel protocol requires a very low amount of plasmid DNA and does not necessitate special laboratory equipment to be performed. Transfection appears to be more efficient in oocysts just triggered for excystation than the excysted sporozoites. Altogether, the application of octaarginine with PEI allows efficient transfection. To the best of our knowledge, this is the first report on an electroporation-free protocol for transfection of sporozoites of a Cryptosporidium species.

scholarly journals Cell-Penetrating Peptide-Functionized Quantum Dots for Intracellular Delivery

2010 ◽  
Vol 10 (12) ◽  
pp. 7897-7905 ◽  
Author(s):  
Betty R. Liu ◽  
Yue-Wern Huang ◽  
Huey-Jenn Chiang ◽  
Han-Jung Lee
Keyword(s):  
Quantum Dots ◽  
Intracellular Delivery ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating
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