Diploid sperm produced by artificially sex-reversed Clone loaches

2007 ◽  
Vol 307A (2) ◽  
pp. 75-83 ◽  
Author(s):  
Hiroyuki Yoshikawa ◽  
Kagayaki Morishima ◽  
Satoshi Kusuda ◽  
Etsuro Yamaha ◽  
Katsutoshi Arai
Keyword(s):  
Diploid Sperm

scholarly journals Increased frequencies of diploid sperm detected by multicolour FISH after treatment of rats with carbendazim without micronucleus induction in peripheral blood erythrocytes

Mutagenesis ◽  
1999 ◽  
Vol 14 (6) ◽  
pp. 621-632 ◽  
Author(s):  
J. M. de Stoppelaar ◽  
T. van de Kuil ◽  
M. Bedaf ◽  
H. W. Verharen ◽  
W. Slob ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Micronucleus Induction ◽  
Multicolour Fish ◽  
Diploid Sperm

scholarly journals Partial molar pregnancy after intracytoplasmic sperm injection occurring as a result of diploid sperm usage

2013 ◽  
Vol 30 (6) ◽  
pp. 761-764 ◽  
Author(s):  
Philip Savage ◽  
Neil Sebire ◽  
Tom Dalton ◽  
Anna Carby ◽  
Michael J. Seckl ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Molar Pregnancy ◽  
Diploid Sperm

Clonal diploid sperm of the diploid-triploid mosaic loach,Misgurnus anguillicaudatus (Teleostei:Cobitidae)

2004 ◽  
Vol 301A (6) ◽  
pp. 502-511 ◽  
Author(s):  
Kagayaki Morishima ◽  
Kouzou Oshima ◽  
Shin Horie ◽  
Takafumi Fujimoto ◽  
Etsuro Yamaha ◽  
...  
Keyword(s):  
Misgurnus Anguillicaudatus ◽  
Diploid Sperm

scholarly journals Double copulation of a female with sterile diploid and polyploid males recovers fertility in Bombyx mori

Zygote ◽  
2002 ◽  
Vol 10 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Ken Sahara ◽  
Naoko Kawamura
Keyword(s):  
Heat Shock ◽  
Microscopic Observation ◽  
Experimental System ◽  
Reproductive Organs ◽  
Sperm Function ◽  
Apyrene Sperm ◽  
Eupyrene Sperm ◽  
Female Reproductive Organs ◽  
Diploid Sperm

Silkworm males produce dimorphic sperm, nucleate eupyrene sperm and anucleate apyrene sperm. Apyrene sperm have been speculated to have an assisting role in fertilisation. However, the coexistence of eupyrene and apyrene sperm in the testis and female reproductive organs has made it difficult to define the role of apyrene sperm. Polyploid males are highly sterile. Microscopic observation revealed that the elimination of eupyrene nuclei by peristaltic squeezing caused the sterility of polyploids. Heat-shock applied to pupae of Daizo males (DH) also induced high sterility due to the lack of normal apyrene sperm. When eupyrene sperm of sterile DH males and apyrene sperm of sterile polyploid males were mixed by double copulation, a remarkable increase in fertility of the double-mated females was observed. This finding strongly suggests that the apyrene sperm are indispensable in fertilisation of the silkworm and that polyploid apyrene sperm function as a substitute for diploid sperm. We established an experimental system in which we can separate the two types of sperm for further studies on their functions without chemical and/or mechanical treatments.

Analysis of aneuploidy for chromosomes 13, 21, X and Y by multicolour fluorescence in situ hybridisation (FISH) in a 47,XYY male

Zygote ◽  
1999 ◽  
Vol 7 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Renée H. Martin ◽  
Brenda McInnes ◽  
A.W. Rademaker
Keyword(s):  
Y Chromosome ◽  
Sex Chromosomes ◽  
In Situ Hybridisation ◽  
Chromosome 1 ◽  
Fluorescence In Situ Hybridisation ◽  
Chromosome 13 ◽  
Normal Men ◽  
Diploid Sperm

The frequency of aneuploid sperm was assessed by fluorescence in situ hybridisation (FISH) in a 47,XYY male previously studied by sperm karyotyping. A total of 20 021 sperm were studied: 10 017 by two-colour FISH for chromosomes 13 and 21 and 10 002 by three-colour FISH for the sex chromosomes using chromosome 1 as an autosomal control for diploidy and lack of hybridisation. Results were compared with more than 500 000 sperm from 18 normal men. The frequencies of X-bearing (49.4%) and Y-bearing sperm (49.8%) were not significantly different from 50% as shown in our sperm karyotyping study. There was no significant increase in the frequency of diploid sperm compared with control donors. There was a significant increase in the frequency of disomy for chromosome 13 (p<0.0001) and XY disomy (p=0.0008) compared with control donors. However, since the frequency of disomy was 0.40% for chromosome 13 and 0.55% for XY disomy, it is not surprising that these increases were not discovered previously in our analysis of 75 sperm karyotypes. Our results suggest that the extra Y chromosome is eliminated during spermatogenesis in the majority of cells but that there may be a small but significant increase in the frequency of aneuploid sperm in these men.

scholarly journals A human tetraploid pachytene spermatocyte as the possible origin of diploid sperm: a case report

2006 ◽  
Vol 21 (7) ◽  
pp. 1795-1797 ◽  
Author(s):  
M. Codina-Pascual ◽  
J. Navarro ◽  
J. Egozcue ◽  
J. Benet
Keyword(s):  
Case Report ◽  
Pachytene Spermatocyte ◽  
Diploid Sperm

Analysis of the reproductive potential of diploid males in the wasp Diadromus pulchellus (Hymenoptera: Ichneumonidae)

1994 ◽  
Vol 84 (2) ◽  
pp. 213-218 ◽  
Author(s):  
M. El Agoze ◽  
J. M. Drezen ◽  
S. Renault ◽  
G. Periquet
Keyword(s):  
High Mortality ◽  
Developmental Stages ◽  
Reproductive Potential ◽  
Diploid Males ◽  
Diadromus Pulchellus ◽  
Laboratory Populations ◽  
Normal Potential ◽  
Diploid Sperm

AbstractIn the ichneumonid wasp Diadromus pulchellus (Wesmael), diploid males may regularly be produced in laboratory populations by inbreeding. They are viable, produce diploid sperm and are equally capable of multiple inseminations as are haploid males. Their spermatozoa are also able to penetrate the oocyte. Progeny sired by diploid males are essentially haploid sons with only a few daughters. The sons are normal haploid individuals, produced in a frequency significantly higher than in crosses between females and haploid males. The daughters are normal diploid females probably resulting from the fertilization of an oocyte by an exceptional haploid sperm rarely produced by diploid males. Triploid individuals were not observed and their absence was correlated with the high mortality observed during the first developmental stages. The death of these individuals is discussed in the light of the phenomenon of superparasitism to explain the increase in the frequency of sons produced in crosses with diploid males. Despite their viability, their ability to produce sperm and their normal potential insemination, diploid males of Diadromus pulchellus are therefore almost sterile.

scholarly journals Restoration of the loach, Misgurnus anguillicaudatus, from cryopreserved diploid sperm and induced androgenesis

Aquaculture ◽  
2010 ◽  
Vol 308 ◽  
pp. S140-S144 ◽  
Author(s):  
George Shigueki Yasui ◽  
Takafumi Fujimoto ◽  
Katsutoshi Arai
Keyword(s):  
Misgurnus Anguillicaudatus ◽  
Diploid Sperm

scholarly journals Diploid sperm and the origin of triploidy

2002 ◽  
Vol 17 (1) ◽  
pp. 5-7 ◽  
Author(s):  
S. Egozcue
Keyword(s):  
Diploid Sperm
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