L-proline transport by purified cell types of lobster hepatopancreas

2006 ◽  
Vol 305A (10) ◽  
pp. 851-861 ◽  
Author(s):  
L. Fiandra ◽  
P.K. Mandal ◽  
B. Giordana ◽  
G.A. Ahearn
Keyword(s):  
Cell Types ◽  
Proline Transport

L-Proline Transport Systems of Starfish Pyloric Caeca

1991 ◽  
Vol 158 (1) ◽  
pp. 477-493 ◽  
Author(s):  
GREGORY A. AHEARN ◽  
RACHEL D. BEHNKE
Keyword(s):  
Amino Acid ◽  
Membrane Vesicles ◽  
Cell Types ◽  
Competitive Inhibitor ◽  
Transport Systems ◽  
Proline Transport ◽  
Sigmoidal Functions ◽  
L System ◽  
Carrier Mediated Transport ◽  
Dependent Transport

Purified brush-border membrane vesicles (BBMV) of starfish [Pycnopodia helianthoides (Brandt)] pyloric caecal epithelium were prepared by magnesium precipitation in order to characterize the possible role of this organ in amino acid transport. L-[3H]proline uptake by these vesicles was Na+-dependent and greater at pH7.5 than at pH5.5. L-Pipecolate was a competitive inhibitor of L-proline influx into these BBMV, exhibiting a Ki value of 0.02 mmol l−1. The amino acid inhibitors, L-pipecolate, L-alanine and L-leucine were used as test substrates to block L-prohne influx by the IMINO, NBB and L transport systems, respectively, in order to estimate the contribution of each process to total L-prohne entry into pyloric caecal cells. The carrier-mediated transport constants for L-prohne transfer by these three systems were: Kt=0.18mmoll−1 (IMINO), 0.13mmol l−1 (NBB) and (0.21mmol l−1 (L); Jmax= 1310 pmol mg−1 protein 30 s−1 (IMINO), 360 pmol mg−1 protein 30 s−1 (NBB) and 470pmol mg−1 protein 30s−1 (L). L-Proline influxes through both the IMINO and NBB systems were sigmoidal functions of the external [Na+], while transfer by the L system was Na+-independent. Multiple sodium ions (e.g. 2 or 3 Na+/L-proline) appear to be associated with L-proline transport by both Na+-dependent transport systems, but the nature of this association (i.e. activation or energization) is unclear. Results suggest that starfish pyloric caecal epithelium possesses a similar array of L-proline transport proteins to those found in similar cell types of mammalian intestine or kidney, providing tentative support for an absorptive function for this organ.

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