Gill area, permeability and Na+,K+-ATPase activity as a function of size and salinity in the blue crab,Callinectes sapidus

2006 ◽  
Vol 305A (3) ◽  
pp. 233-245 ◽  
Author(s):  
Tiandao Li ◽  
Robert Roer ◽  
Matthew Vana ◽  
Susan Pate ◽  
Jennifer Check
Keyword(s):  
Atpase Activity ◽  
Blue Crab ◽  
Callinectes Sapidus ◽  
Gill Area

scholarly journals D1-like dopamine receptors downregulate Na+-K+-ATPase activity and increase cAMP production in the posterior gills of the blue crab Callinectes sapidus

2014 ◽  
Vol 307 (6) ◽  
pp. R634-R642 ◽  
Author(s):  
Francis B. Arnaldo ◽  
Van Anthony M. Villar ◽  
Prasad R. Konkalmatt ◽  
Shaun A. Owens ◽  
Laureano D. Asico ◽  
...  
Keyword(s):  
Protein Expression ◽  
Atpase Activity ◽  
Dopamine Receptors ◽  
Blue Crab ◽  
Callinectes Sapidus ◽  
Basolateral Membrane ◽  
Camp Production ◽  
Degenerate Primers ◽  
Α Subunit ◽  
Low Salinity

Dopamine-mediated regulation of Na+-K+-ATPase activity in the posterior gills of some crustaceans has been reported to be involved in osmoregulation. The dopamine receptors of invertebrates are classified into three groups based on their structure and pharmacology: D1- and D2-like receptors and a distinct invertebrate receptor subtype (INDR). We tested the hypothesis that a D1-like receptor is expressed in the blue crab Callinectes sapidus and regulates Na+-K+-ATPase activity. RT-PCR, using degenerate primers, showed the presence of D1βR mRNA in the posterior gill. The blue crab posterior gills showed positive immunostaining for a dopamine D5 receptor (D5R or D1βR) antibody in the basolateral membrane and cytoplasm. Confocal microscopy showed colocalization of Na+-K+-ATPase and D1βR in the basolateral membrane. To determine the effect of D1-like receptor stimulation on Na+-K+-ATPase activity, intact crabs acclimated to low salinity for 6 days were given an intracardiac infusion of the D1-like receptor agonist fenoldopam, with or without the D1-like receptor antagonist SCH23390. Fenoldopam increased cAMP production twofold and decreased Na+-K+-ATPase activity by 50% in the posterior gills. This effect was blocked by coinfusion with SCH23390, which had no effect on Na+-K+-ATPase activity by itself. Fenoldopam minimally decreased D1βR protein expression (10%) but did not affect Na+-K+-ATPase α-subunit protein expression. This study shows the presence of functional D1βR in the posterior gills of euryhaline crabs chronically exposed to low salinity and highlights the evolutionarily conserved function of the dopamine receptors on sodium homeostasis.

Foraging behavior of an estuarine predator, the blue crab Callinectes sapidus in a patchy environment

Ecography ◽  
2000 ◽  
Vol 23 (1) ◽  
pp. 21-31 ◽  
Author(s):  
Mary E. Clark ◽  
Thomas G. Wolcott ◽  
Donna L. Wolcott ◽  
Anson H. Hines
Keyword(s):  
Foraging Behavior ◽  
Blue Crab ◽  
Callinectes Sapidus ◽  
Patchy Environment
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