The anterior midgut of larval yellow fever mosquitoes (Aedes aegypti): effects of amino acids, dicarboxylic acids, and glucose on the transepithelial voltage and strong luminal alkalinization

2009 ◽  
Vol 311A (9) ◽  
pp. 719-726 ◽  
Author(s):  
Sejmir Izeirovski ◽  
Stacia B. Moffett ◽  
David F. Moffett ◽  
Horst Onken
Keyword(s):  
Amino Acids ◽  
Aedes Aegypti ◽  
Yellow Fever ◽  
Dicarboxylic Acids ◽  
Transepithelial Voltage ◽  
Anterior Midgut

scholarly journals Repellent activity of monoterpenoid esters with neurotransmitter amino acids against yellow fever mosquito, Aedes aegypti

2018 ◽  
Vol 16 (1) ◽  
pp. 95-98 ◽  
Author(s):  
Mariia Nesterkina ◽  
Ulrich R. Bernier ◽  
Nurhayat Tabanca ◽  
Iryna Kravchenko
Keyword(s):  
Amino Acids ◽  
Aedes Aegypti ◽  
Yellow Fever ◽  
Prolonged Exposure ◽  
Repellent Activity ◽  
Reference Standard ◽  
Yellow Fever Mosquito ◽  
Neurotransmitter Amino Acids

AbstractRepellent activity of monoterpenoid esters (1-6) with neurotransmitter amino acids (GABA and glycine) was investigated against Aedes aegypti by using a “cloth-patch” assay and compared to reference standard N,N-diethyl-3-methylbenzamide (DEET). Monoterpenoid esters showed repellent activity with minimum effective dosages (MED) in the range of 0.031-0.469 mg/cm2. The carvacrol ester of GABA (2, MED of 0.031 ± 0.008 mg/cm2) exhibited the highest repellency of six monoterpenoid esters tested in comparison to the standard repellent DEET (MED of 0.009 ± 0.002 mg/cm2); however, the repellent activity of carvacrol-glycine ester (5) decreased 4-fold compared to the carvacrol-GABA derivative (2). The repellent activities of menthol GABA (1, MED= 0.375 ± 0.000 mg/cm2) and glycine ester (4, MED=0.312 ± 0.063 mg/cm2) were similar The guaiacol-glycine ester (6) was 3.75-fold more efficacious than the guaiacol ester of GABA (3). In the present study, we report repellent efficacy of prolonged exposure to GABA and glycine esters of menthol, carvacrol, guaiacol (1-6) as compared to the repellent activities of their monoterpene moieties alone.

scholarly journals Strong alkalinization in the anterior midgut of larval yellow fever mosquitoes (Aedes aegypti): involvement of luminal Na+/K+-ATPase

2009 ◽  
Vol 311A (3) ◽  
pp. 155-161 ◽  
Author(s):  
Horst Onken ◽  
Malay Patel ◽  
Margarita Javoroncov ◽  
Sejmir Izeirovski ◽  
Stacia B. Moffett ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Anterior Midgut

Dibutyryl cAMP activates bumetanide-sensitive electrolyte transport in Malpighian tubules

1991 ◽  
Vol 261 (3) ◽  
pp. C521-C529 ◽  
Author(s):  
J. L. Hegarty ◽  
B. Zhang ◽  
T. L. Pannabecker ◽  
D. H. Petzel ◽  
M. D. Baustian ◽  
...  
Keyword(s):  
Yellow Fever ◽  
Apical Membrane ◽  
Basolateral Membrane ◽  
Fluid Secretion ◽  
Ringer Solution ◽  
Malpighian Tubules ◽  
Membrane Voltage ◽  
Dibutyryl Camp ◽  
K Secretion ◽  
Transepithelial Voltage

The effects of dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) and bumetanide (both 10(-4) M) on transepithelial Na+, K+, Cl-, and fluid secretion and on tubule electrophysiology were studied in isolated Malpighian tubules of the yellow fever mosquito Aedes aegypti. Peritubular DBcAMP significantly increased Na+, Cl-, and fluid secretion but decreased K+ secretion. In DBcAMP-stimulated tubules, bumetanide caused Na+, Cl-, and fluid secretion to return to pre-cAMP control rates and K+ secretion to decrease further. Peritubular bumetanide significantly increased Na+ secretion and decreased K+ secretion so that Cl- and fluid secretion did not change. In bumetanide-treated tubules, the secretagogue effects of DBcAMP are blocked. In isolated Malpighian tubules perfused with symmetrical Ringer solution, DBcAMP significantly hyperpolarized the transepithelial voltage (VT) and depolarized the basolateral membrane voltage (Vbl) with no effect on apical membrane voltage (Va). Total transepithelial resistance (RT) and the fractional resistance of the basolateral membrane (fRbl) significantly decreased. Bumetanide also hyperpolarized VT and depolarized Vbl, however without significantly affecting RT and fRbl. Together these results suggest that, in addition to stimulating electroconductive transport, DBcAMP also activates a nonconductive bumetanide-sensitive transport system in Aedes Malpighian tubules.

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