Beyond corticosterone: The acute stress response increases DNA damage in house sparrows

AbstractDisturbed activation or regulation of the stress response through the hypothalamic-pituitary-adrenal (HPA) axis is a fundamental component of multiple stress-related diseases, including psychiatric, metabolic, and immune disorders. The FK506 binding protein 51 (FKBP5) is a negative regulator of the glucocorticoid receptor (GR), the main driver of HPA axis regulation, and FKBP5 polymorphisms have been repeatedly linked to stress-related disorders in humans. However, the specific role of Fkbp5 in the paraventricular nucleus of the hypothalamus (PVN) in shaping HPA axis (re)activity remains to be elucidated. We here demonstrate that the deletion of Fkbp5 in Sim1+ neurons dampens the acute stress response and increases GR sensitivity. In contrast, Fkbp5 overexpression in the PVN results in a chronic HPA axis over-activation, and a PVN-specific rescue of Fkbp5 expression in full Fkbp5 KO mice normalizes the HPA axis phenotype. Single-cell RNA sequencing revealed the cell-type-specific expression pattern of Fkbp5 in the PVN and showed that Fkbp5 expression is specifically upregulated in Crh+ neurons after stress. Finally, Crh-specific Fkbp5 overexpression alters Crh neuron activity, but only partially recapitulates the PVN-specific Fkbp5 overexpression phenotype. Together, the data establish the central and cell-type-specific importance of Fkbp5 in the PVN in shaping HPA axis regulation and the acute stress response.

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Machine Learning for personalised stress detection: Inter-individual variability of EEG-ECG markers for acute-stress response

Computer Methods and Programs in Biomedicine ◽

10.1016/j.cmpb.2021.106314 ◽

2021 ◽

pp. 106314

Author(s):

L. Gonzalez-Carabarin ◽

E.A. Castellanos-Alvarado ◽

P. Castro-Garcia ◽

M.A. Garcia-Ramirez

Keyword(s):

Machine Learning ◽

Stress Response ◽

Acute Stress ◽

Individual Variability ◽

Stress Detection ◽

Acute Stress Response

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Acute stress response modified by modest inhibition of growth hormone axis: A potential machinery of the anti-aging effect of calorie restriction

Mechanisms of Ageing and Development ◽

10.1016/j.mad.2011.01.002 ◽

2011 ◽

Vol 132 (3) ◽

pp. 103-109 ◽

Cited By ~ 3

Author(s):

Toshimitsu Komatsu ◽

Lucas S. Trindade ◽

Takuya Chiba ◽

Hiroko Hayashi ◽

Tomoko Henmi ◽

...

Keyword(s):

Growth Hormone ◽

Stress Response ◽

Calorie Restriction ◽

Acute Stress ◽

Aging Effect ◽

Inhibition Of Growth ◽

Acute Stress Response

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Effects of acetylsalicylic acid treatment on thyroid hormones, prolactins, and the stress response of tilapia (Oreochromis mossambicus)

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00731.2002 ◽

2003 ◽

Vol 285 (5) ◽

pp. R1098-R1106 ◽

Cited By ~ 20

Author(s):

Rogier D. van Anholt ◽

Tom Spanings ◽

William Koven ◽

Sjoerd E. Wendelaar Bonga

Keyword(s):

Stress Response ◽

Acetylsalicylic Acid ◽

Acute Stress ◽

Oreochromis Mossambicus ◽

Control Fish ◽

Mild Stress ◽

Cox Inhibitor ◽

Basal Plasma ◽

Acute Stress Response

The cyclooxygenase (COX) pathway converts arachidonic acid (ArA) into prostaglandins (PGs), which interact with the stress response in mammals and possibly in fish as well. Acetylsalicylic acid (ASA) is a COX inhibitor and was used to characterize the effects of PGs on the release of several hormones and the stress response of tilapia ( Oreochromis mossambicus). Plasma PGE2 was significantly reduced at 100 mg ASA/kg body wt, and both basal PGE2 and cortisol levels correlated negatively with plasma salicylate. Basal plasma 3,5,3′-triiodothyronine (T3) was reduced by ASA treatment, whereas prolactin (PRL)188 increased at 100 mg ASA/kg body wt. ASA depressed the cortisol response to the mild stress of 5 min of net confinement. As expected, glucose and lactate were elevated in the stressed control fish, but the responses were blunted by ASA treatment. Gill Na+-K+-ATPase activity was not affected by ASA. Plasma osmolarity increased after confinement in all treatments, whereas sodium only increased at the high ASA dose. This is the first time ASA has been administered to fish in vivo, and the altered hormone release and the inhibition of the acute stress response indicated the involvement of PGs in these processes.

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