Ubiquitin and actin expression in claw muscles of land crab,Gecarcinus lateralis, and american lobster,Homarus americanus: Differential expression of ubiquitin in two slow muscle fiber types during molt-induced atrophy

2002 ◽  
Vol 292 (7) ◽  
pp. 618-632 ◽  
Author(s):  
Annette Koenders ◽  
Xiaoli Yu ◽  
Ernest S. Chang ◽  
Donald L. Mykles
Keyword(s):  
Differential Expression ◽  
Muscle Fiber ◽  
Homarus Americanus ◽  
Slow Muscle ◽  
Muscle Fiber Types ◽  
American Lobster ◽  
Fiber Types ◽  
Land Crab ◽  
Gecarcinus Lateralis ◽  
Actin Expression

scholarly journals OR-043 UPRmt and mitophagy are selectively activated depending on muscle fiber types in insulin resistant rats

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Can Li ◽  
Nan Li ◽  
Yong Zhang
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Quality Control ◽  
Muscle Fiber ◽  
Slow Muscle ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Mitochondrial Quality Control ◽  
Insulin Resistant ◽  
Related Proteins

Objective To investigate how different skeletal muscle fiber types affect development of insulin resistance, and to explore the role of mitochondrial quality control system, especially mitochondrial unfolded protein response (UPRmt) and mitophagy, in response to metabolic stresses. Methods Male Wistar rats were randomly divided into 2 groups: fed with the normal diet for 8 weeks (Con), and fed with 45% high-fat diet for 8 weeks (IR). Fasting blood glucose (FBG), fasting insulin (FIN) and oral glucose tolerance test (OGTT) were used to identify insulin resistance model. Gastrocnemius (GC), soleus (SOL) and tibialis anterior (TA) muscle were isolated, and RT-qPCR was used to determine the expression of Myhc7, Myhc4. Oxygraph-2k was used to determine the mitochondrial State 3 (ST3), State 4(ST4) respiration and respiration control rate (RCR). JC-1 probe was used to measure mitochondrial membrane potential. Western Blot was used to determine the expressions of marker proteins of muscle fiber types (Myhc4, Myhc7), UPRmt related proteins (Hsp60, Hsp70) and mitophagy related proteins (Pink1, LC3). Results Compared with Con group, in IR group, FBG (7.1±1.27 vs. 5.4±0.43，p＜0.05), FIN (19.4±5.2 vs. 31.6±6.7，p＜0.05 ) and OGTT (area under the curve, about 31.7% increases, p＜0.05) were significantly higher. Myhc4 mRNA (relative fold about 55.6% increases) and protein expression (about 33.9% increases, p＜0.05) were significantly higher in GC. Myhc4 protein expression was significantly higher in GC (about 60.5% increases, p＜0.05). While Myhc7 mRNA expression (about 51.1% decreases, p＜0.05) was significantly lower in SOL. Compared with Con group, in IR group, mitochondrial RCR in SOL muscle was significantly lower (about 22.5% decreases, p＜0.05). Furthermore, the expression of HSP60 (about 36.7% increases，p＜0.05) and HSP70 (about 44.3% increases，p＜0.05) was significantly higher in TA muscle, while the expression of Parkin (about 18.8% decreases，p＜0.05) and the ratio between LC3 II/I (about 26.0% decreases，p＜0.05)expression in SOL muscle were significantly lower. Conclusions In this study, we found that the percentage of fast muscle fibers was elevated in IR skeletal muscle, which were supported by increased Myhc4 and decreased Myhc7 level. Impaired mitochondrial function was only observed in slow muscle as inhibition of mitochondrial respiration. As marker of UPRmt, HSP60/70 were specifically activated in fast muscle in IR, while mitophagy-related proteins were specifically increased in slow muscle. These results indicate that mitochondrial quality control systems are selectively activated to recover mitochondrial functions depending on muscle fiber types in insulin resistant rat.

Qualitatively different cross-bridge attachments in fast and slow muscle fiber types

2009 ◽  
Vol 385 (1) ◽  
pp. 44-48 ◽  
Author(s):  
Stefan Galler ◽  
Oleg Andruchov ◽  
Gabriela M.M. Stephenson ◽  
D. George Stephenson
Keyword(s):  
Muscle Fiber ◽  
Slow Muscle ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Fast And Slow Muscle ◽  
Cross Bridge

A new variant of proline-rich membrane anchor (PRiMA) of acetylcholinesterase in chicken: Expression in different muscle fiber types

2009 ◽  
Vol 461 (2) ◽  
pp. 202-206 ◽  
Author(s):  
Mokka K.W. Mok ◽  
Ka W. Leung ◽  
Heidi Q. Xie ◽  
Ava J.Y. Guo ◽  
Vicky P. Chen ◽  
...  
Keyword(s):  
Muscle Fiber ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Membrane Anchor ◽  
New Variant

Muscle fiber types composition and type identified endplate morphology of forepaw intrinsic muscles in the rat

2016 ◽  
Vol 37 (3) ◽  
pp. 95-100
Author(s):  
Feng Pan ◽  
Jing-yi Mi ◽  
Yan Zhang ◽  
Xiao-yun Pan ◽  
Yong-jun Rui
Keyword(s):  
Muscle Fiber ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Intrinsic Muscles

Blood flow to different skeletal muscle fiber types during contraction

1983 ◽  
Vol 245 (2) ◽  
pp. H265-H275 ◽  
Author(s):  
B. G. Mackie ◽  
R. L. Terjung
Keyword(s):  
Skeletal Muscle ◽  
Blood Flow ◽  
Muscle Fiber ◽  
Fiber Type ◽  
Oxidative Capacity ◽  
Skeletal Muscle Fiber ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Blood Flows ◽  
Fast Twitch

Blood flow to fast-twitch red (FTR), fast-twitch white (FTW), and slow-twitch red (STR) muscle fiber sections of the gastrocnemius-plantaris-soleus muscle group was determined using 15 +/- 3-microns microspheres during in situ stimulation in pentobarbital-anesthetized rats. Steady-state blood flows were assessed during the 10th min of contraction using twitch (0.1, 0.5, 1, 3, and 5 Hz) and tetanic (7.5, 15, 30, 60, and 120/min) stimulation conditions. In addition, an earlier blood flow determination was begun at 3 min (twitch series) or at 30 s (tetanic series) of stimulation. Blood flow was highest in the FTR (220-240 ml X min-1 X 100 g-1), intermediate in the STR (140), and lowest in the FTW (70-80) section during tetanic contraction conditions estimated to coincide with the peak aerobic function of each fiber type. These blood flows are fairly proportional to the differences in oxidative capacity among fiber types. Further, their absolute values are similar to those predicted from the relationship between blood flow and oxidative capacity found by others for dog and cat muscles. During low-frequency contraction conditions, initial blood flow to the FTR and STR sections were excessively high and not dependent on contraction frequency. However, blood flows subsequently decreased to values in keeping with the relative energy demands. In contrast, FTW muscle did not exhibit this time-dependent relative hyperemia. Thus, besides the obvious quantitative differences between skeletal muscle fiber types, there are qualitative differences in blood flow response during contractions. Our findings establish that, based on fiber type composition, a heterogeneity in blood flow distribution can occur within a whole muscle during contraction.

Sign in / Sign up

Export Citation Format

Share Document