Differential induction of branchial carbonic anhydrase and NA+/K+ ATPase activity in the euryhaline crab,Carcinus maenas, in response to low salinity exposure

2002 ◽  
Vol 292 (7) ◽  
pp. 595-603 ◽  
Author(s):  
Raymond P. Henry ◽  
Elizabeth E. Garrelts ◽  
Melissa M. McCarty ◽  
David W. Towle
Keyword(s):  
Carbonic Anhydrase ◽  
Atpase Activity ◽  
Carcinus Maenas ◽  
Low Salinity ◽  
Differential Induction

Ca++-ATPase activity in the hepatopancreas cells of Oniscus asellus

1992 ◽  
Vol 50 (1) ◽  
pp. 820-821
Author(s):  
G.M. Vernon ◽  
A. Surace ◽  
R. Witkus
Keyword(s):  
B Cells ◽  
Epithelial Cell ◽  
Atpase Activity ◽  
Calcium Transport ◽  
Carcinus Maenas ◽  
Cell Types ◽  
Molt Cycle ◽  
Copper And Zinc

The hepatopancreas consists of a pair of bilobed tubules comprised of two epithelial cell types. S cells are absorptive and accumulate metals such as copper and zinc. Ca++ concentrations vary between the S and B cells and during the molt cycle. Roer and Dillaman implicated Ca++-ATPase in calcium transport during molting in Carcinus maenas. This study was undertaken to compare the localization of Ca++-ATPase activity in the S and B cells during intermolt.

Na+-K+-ATPase and Na+/Ca2+exchange activities in gills of hyperregulatingCarcinus maenas

1999 ◽  
Vol 276 (2) ◽  
pp. R490-R499 ◽  
Author(s):  
Čedomil Lucu ◽  
Gert Flik
Keyword(s):  
Atpase Activity ◽  
Specific Activity ◽  
Short Circuit ◽  
Carcinus Maenas ◽  
High Capacity ◽  
Short Circuit Current ◽  
Total Activity ◽  
Camp Content ◽  
Dependent Protein ◽  
Leaky Epithelium

Na+-K+-ATPase and Na+/Ca2+exchange activities were studied in gills of Carcinus maenas in seawater (SW) and after transfer to dilute seawater (DSW). Carcinushyperregulates its hemolymph osmolarity through active uptake of Na+, Cl−, and Ca2+. In DSW total Na+-K+-ATPase activity in posterior gills quadrupled; Na+/Ca2+exchange specific activity was unaffected, and total activity increased 1.67-fold. Short-circuit current ( Isc) in voltage-clamped posterior gill hemilamellae was −181 μA/cm2in SW and −290 μA/cm2in DSW and up to 90% ouabain sensitive; conductivity was similar in SW or DSW (42 and 46 mS/cm2, respectively) and representative of a leaky epithelium. The new steady state of hemolymph osmolarity 24 h after DSW transfer was preceded, already 3 h after transfer, by increased Na+-K+-ATPase but not Na+/Ca2+exchange activity. Western blot analysis indicated that the amount of Na+-K+-ATPase protein had increased 2.1-fold in crabs acclimated 3 wk to DSW; however, 4 h after DSW transfer no difference in the amount of Na+-K+-ATPase protein was observed. After DSW transfer branchial cAMP content decreased. A negative correlation between branchial Na+-K+-ATPase activity and cAMP content points to rapid regulation of Na+-K+-ATPase through cAMP-dependent protein kinase A activity. Ca2+transport may depend on the high-capacity Na+/Ca2+exchanger coupled to the versatile sodium pump.

scholarly journals D1-like dopamine receptors downregulate Na+-K+-ATPase activity and increase cAMP production in the posterior gills of the blue crab Callinectes sapidus

2014 ◽  
Vol 307 (6) ◽  
pp. R634-R642 ◽  
Author(s):  
Francis B. Arnaldo ◽  
Van Anthony M. Villar ◽  
Prasad R. Konkalmatt ◽  
Shaun A. Owens ◽  
Laureano D. Asico ◽  
...  
Keyword(s):  
Protein Expression ◽  
Atpase Activity ◽  
Dopamine Receptors ◽  
Blue Crab ◽  
Callinectes Sapidus ◽  
Basolateral Membrane ◽  
Camp Production ◽  
Degenerate Primers ◽  
Α Subunit ◽  
Low Salinity

Dopamine-mediated regulation of Na+-K+-ATPase activity in the posterior gills of some crustaceans has been reported to be involved in osmoregulation. The dopamine receptors of invertebrates are classified into three groups based on their structure and pharmacology: D1- and D2-like receptors and a distinct invertebrate receptor subtype (INDR). We tested the hypothesis that a D1-like receptor is expressed in the blue crab Callinectes sapidus and regulates Na+-K+-ATPase activity. RT-PCR, using degenerate primers, showed the presence of D1βR mRNA in the posterior gill. The blue crab posterior gills showed positive immunostaining for a dopamine D5 receptor (D5R or D1βR) antibody in the basolateral membrane and cytoplasm. Confocal microscopy showed colocalization of Na+-K+-ATPase and D1βR in the basolateral membrane. To determine the effect of D1-like receptor stimulation on Na+-K+-ATPase activity, intact crabs acclimated to low salinity for 6 days were given an intracardiac infusion of the D1-like receptor agonist fenoldopam, with or without the D1-like receptor antagonist SCH23390. Fenoldopam increased cAMP production twofold and decreased Na+-K+-ATPase activity by 50% in the posterior gills. This effect was blocked by coinfusion with SCH23390, which had no effect on Na+-K+-ATPase activity by itself. Fenoldopam minimally decreased D1βR protein expression (10%) but did not affect Na+-K+-ATPase α-subunit protein expression. This study shows the presence of functional D1βR in the posterior gills of euryhaline crabs chronically exposed to low salinity and highlights the evolutionarily conserved function of the dopamine receptors on sodium homeostasis.

Histochemical localization of Na+–K+ ATPase and carbonic anhydrase activity in gills of 17 fish species

1988 ◽  
Vol 66 (11) ◽  
pp. 2398-2405 ◽  
Author(s):  
David M. Conley ◽  
Jon Mallatt
Keyword(s):  
Carbonic Anhydrase ◽  
Atpase Activity ◽  
Carbonic Anhydrase Activity ◽  
Chloride Cells ◽  
Shark Species ◽  
Acid Base ◽  
Pavement Cells ◽  
Leopard Shark ◽  
Biochemical Assays ◽  
Definite Correlation

Activities of two enzymes considered to be involved in NaCl regulation, Na+–K+ ATPase and carbonic anhydrase, were localized in gill epithelia of 14 teleost, 2 agnathan, and 1 shark species through light microscopic histochemistry. Findings were confirmed by use of appropriate inhibitors (ouabain, acetazolamide). Na+–K+ ATPase activity was detected in chloride cells of most marine teleost species (six of eight) and of marine leopard shark and hagfish, but never in freshwater fish gills. In general, this finding agrees with past biochemical assays showing gill Na+–K+ ATPase activity to be highest in marine teleosts. Staining for carbonic anhydrase took one of three patterns among species: gill pavement cells or chloride cells, or both, were stained. Interspecific distribution of these patterns bore little relation to taxonomy or to habitat salinity, although chloride cells of euryhaline teleosts seemed more likely to stain than chloride cells of stenohaline teleosts, freshwater or marine. Given the lack of a definite correlation with salinity, it is concluded that fish gill carbonic anhydrase may not function in NaCl regulation as much as in acid–base regulation; the enzyme's role in preventing systemic pH imbalance is discussed.

Carbonic anhydrase in branchial tissues of osmoregulating shore crabs,Carcinus maenas

1990 ◽  
Vol 255 (3) ◽  
pp. 251-261 ◽  
Author(s):  
K. Böttcher ◽  
D. Siebers ◽  
W. Becker
Keyword(s):  
Carbonic Anhydrase ◽  
Carcinus Maenas ◽  
Shore Crabs

Effects of Acid Water and Aluminum on Parr–Smolt Transformation and Seawater Tolerance in Atlantic Salmon, Salmo salar

1993 ◽  
Vol 50 (9) ◽  
pp. 1816-1827 ◽  
Author(s):  
Magne Staurnes ◽  
Per Blix ◽  
Ola B. Reite
Keyword(s):  
Carbonic Anhydrase ◽  
Atlantic Salmon ◽  
Atpase Activity ◽  
Salmo Salar ◽  
Carbonic Anhydrase Activity ◽  
Low Ph ◽  
Soft Water ◽  
Control Fish ◽  
Exposed Fish ◽  
Seawater Tolerance

Smolting Atlantic salmon, Salmo salar, were kept from 11 April to 24 May in soft water of pH 5 or in soft water of pH 5 and 50 μg aluminum (Al)∙L−1. Control fish were kept in soft water of pH 6.3–6.5. Water temperature was 8–14 °C. In mid-May, some of the control smolts were transferred to the test conditions for 8 d. Exposure to acid water resulted in osmoregulatory failure and high mortality rate. Al strongly enhanced toxicity. Sensitivity to low pH or low pH/Al exposure greatly increased when fish had developed to seawater tolerant smolts. In control and acid-exposed fish, gill carbonic anhydrase activity remained unchanged throughout the experiment whereas in Al-exposed fish, carbonic anhydrase activity decreased. Gill Na+K+-ATPase activity in control fish peaked in mid-May simulanteously with development of seawater tolerance. Fish from both acid-exposed groups had low seawater tolerance. Na+,K+-ATPase activity declined to 60% of start value in acid-exposed fish and to parr level in Al-exposed fish. Hypoosmoregulatory ability was linearly correlated with gill Na+K+-ATPase activity. Reduction in plasma Na+ concentration in acid-exposed fish was linearly correlated with the reduction in gill Na+,K+-ATPase activity.

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