scholarly journals Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV‐CATCHER): A customizable purification assay designed for small‐RNA biomarker identification and evaluation of circulating small‐EVs

2021 ◽  
Vol 10 (8) ◽  
Author(s):  
Megan I. Mitchell ◽  
Iddo Z. Ben‐Dov ◽  
Christina Liu ◽  
Kenny Ye ◽  
Kar Chow ◽  
...  
Keyword(s):  
Small Rna ◽  
Extracellular Vesicle ◽  
Biomarker Identification

scholarly journals Emerging methods in biomarker identification for extracellular vesicle‐based liquid biopsy

2021 ◽  
Vol 10 (7) ◽  
Author(s):  
Yaxuan Liang ◽  
Brandon M. Lehrich ◽  
Siyang Zheng ◽  
Mengrou Lu
Keyword(s):  
Liquid Biopsy ◽  
Extracellular Vesicle ◽  
Biomarker Identification

scholarly journals Heterogeneity and interplay of the extracellular vesicle small RNA transcriptome and proteome

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Helena Sork ◽  
Giulia Corso ◽  
Kaarel Krjutskov ◽  
Henrik J. Johansson ◽  
Joel Z. Nordin ◽  
...  
Keyword(s):  
Small Rna ◽  
Extracellular Vesicle

scholarly journals Detection of Atherosclerosis by Small RNA-Sequencing Analysis of Extracellular Vesicle Enriched Serum Samples

2021 ◽  
Vol 9 ◽  
Author(s):  
Alex Hildebrandt ◽  
Benedikt Kirchner ◽  
Agnes S. Meidert ◽  
Florian Brandes ◽  
Anja Lindemann ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Carotid Artery ◽  
Small Rna ◽  
Mrna Level ◽  
Arterial Disease ◽  
Extracellular Vesicle ◽  
Small Rna Sequencing ◽  
Peripheral Arterial ◽  
Overrepresentation Analysis

Atherosclerosis can occur throughout the arterial vascular system and lead to various diseases. Early diagnosis of atherosclerotic processes and of individual disease patterns would be more likely to be successful if targeted therapies were available. For this, it is important to find reliable biomarkers that are easily accessible and with little inconvenience for patients. There are many cell culture, animal model or tissue studies that found biomarkers at the microRNA (miRNA) and mRNA level describing atherosclerotic processes. However, little is known about their potential as circulating and liquid biopsy markers in patients. In this study, we examined serum-derived miRNA – profiles from 129 patients and 28 volunteers to identify potential biomarkers. The patients had four different atherosclerotic manifestations: abdominal aneurysm (n = 35), coronary heart disease (n = 34), carotid artery stenosis (n = 24) and peripheral arterial disease (n = 36). The samples were processed with an extracellular vesicle enrichment protocol, total-RNA extraction and small RNA-sequencing were performed. A differential expression analysis was performed bioinformatically to find potentially regulated miRNA biomarkers. Resulting miRNA candidates served as a starting point for an overrepresentation analysis in which relevant target mRNAs were identified. The Gene Ontology database revealed relevant biological functions in relation to atherosclerotic processes. In patients, expression of specific miRNAs changed significantly compared to healthy volunteers; 27 differentially expressed miRNAs were identified. We were able to detect a group-specific miRNA fingerprint: miR-122-5p, miR-2110 and miR-483-5p for abdominal aortic aneurysm, miR-370-3p and miR-409-3p for coronary heart disease, miR-335-3p, miR-381-3p, miR493-5p and miR654-3p for carotid artery stenosis, miR-199a-5p, miR-215-5p, miR-3168, miR-582-3p and miR-769-5p for peripheral arterial disease. The results of the study show that some of the identified miRNAs have already been associated with atherosclerosis in previous studies. Overrepresentation analysis on this data detected biological processes that are clearly relevant for atherosclerosis, its development and progression showing the potential of these miRNAs as biomarker candidates. In a next step, the relevance of these findings on the mRNA level is to be investigated and substantiated.

scholarly journals Ozone‐induced changes in the murine lung extracellular vesicle small RNA landscape

2021 ◽  
Vol 9 (18) ◽  
Author(s):  
Gregory J. Smith ◽  
Adelaide Tovar ◽  
Matt Kanke ◽  
Yong Wang ◽  
Jessy S. Deshane ◽  
...  
Keyword(s):  
Small Rna ◽  
Extracellular Vesicle ◽  
Murine Lung ◽  
Induced Changes

Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine

2016 ◽  
Vol 429 ◽  
pp. 39-49 ◽  
Author(s):  
Rachel E. Crossland ◽  
Jean Norden ◽  
Louis A. Bibby ◽  
Joanna Davis ◽  
Anne M. Dickinson
Keyword(s):  
Small Rna ◽  
Rna Isolation ◽  
Extracellular Vesicle ◽  
Qrt Pcr ◽  
Serum And Urine

scholarly journals Assessment of methods for serum extracellular vesicle small RNA sequencing to support biomarker development

2019 ◽  
Vol 8 (1) ◽  
pp. 1684425 ◽  
Author(s):  
Swetha Srinivasan ◽  
Manuel X. Duval ◽  
Vivek Kaimal ◽  
Carolyn Cuff ◽  
Stephen H. Clarke
Keyword(s):  
Rna Sequencing ◽  
Small Rna ◽  
Extracellular Vesicle ◽  
Small Rna Sequencing

scholarly journals Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis

2021 ◽  
Vol 12 ◽  
Author(s):  
Geng Lu ◽  
XinRui Jiang ◽  
Anni Wu ◽  
Jiawei Zhou ◽  
Hengjun Liu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Extracellular Vesicles ◽  
Small Rna ◽  
Membrane Vesicles ◽  
Cell Types ◽  
Extracellular Vesicle ◽  
Diagnostic Biomarkers ◽  
Tuberculosis Patients ◽  
Active Pulmonary Tuberculosis ◽  
Novel Approach

The rapid diagnosis of tuberculosis (TB) is of great significance for the control and treatment of TB. However, TB remains a major healthy, social, and economic burden worldwide because of the lack of ideal diagnostic biomarkers. Mycobacterium tuberculosis (M. tuberculosis)-encoded small RNA (sRNA) is a class of regulation small RNA. Several studies have identified M. tuberculosis encoded-sRNAs in the serum/plasm of M. tuberculosis-infected patients. Small extracellular vesicles are small membrane vesicles secreted by many cell types during physiological and pathological conditions. Recent evidence has indicated that most of the nucleic acids in the serum/plasma are packaged in the small extracellular vesicles and could serve as ideal diagnostic biomarkers. In this study, we attempted a novel approach for TB diagnosis: targeting small extracellular vesicles M. tuberculosis encoded sRNA (sRNA) by qRT-PCR. The results showed that M. tuberculosis-encoded ASdes and MTB-miR5 only existed in tuberculosis patients and have the potential to serve as a sensitive and accurate methodology for TB diagnosis.

scholarly journals MicroRNA composition delineates cardiosphere-derived cell and mesenchymal stem cell extracellular vesicles

2020 ◽  
Author(s):  
Ann-Sophie Walravens ◽  
Sasha Smolgovsky ◽  
Liang Li ◽  
Lauren Kelly ◽  
Travis Antes ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cells ◽  
Immune Response ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Small Rna ◽  
Cell Types ◽  
Extracellular Vesicle ◽  
Small Rna Sequencing ◽  
Parent Cell

AbstractCell therapy limits ischemic injury following myocardial infarction (MI) by limiting cell death, modulating the immune response, and promoting tissue regeneration. The therapeutic efficacy of cardiosphere-derived cells (CDCs) and mesenchymal stem cells (MSCs) is associated with extracellular vesicle (EV) release. Despite differences in cell origin, it is unclear why EVs elicit differences in therapeutic potency between cell types. Here, we compare EVs derived from multiple MSC and CDC donors. We reveal that EV membrane protein and microRNA (miR) composition are reflective of their parent cell. Small RNA-sequencing revealed enrichment of miR-10b in MSC EVs. Our data support the hypothesis that CDC EVs are distinct from MSC-EVs and is reflected by their miR composition.

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